EP 2021499 A4 20100217 - FUNCTIONAL ARRAYS FOR HIGH THROUGHPUT CHARACTERIZATION OF GENE EXPRESSION REGULATORY ELEMENTS
Title (en)
FUNCTIONAL ARRAYS FOR HIGH THROUGHPUT CHARACTERIZATION OF GENE EXPRESSION REGULATORY ELEMENTS
Title (de)
FUNKTIONELLE ARRAYS ZUR KENNZEICHNUNG VON ELEMENTEN ZUR GENEXPRESSIONSREGULIERUNG MIT HOHEM DURCHSATZ
Title (fr)
RESEAUX FONCTIONNELS POUR LA CARACTERISATION A GRANDE CADENCE D'ELEMENTS REGULANT L'EXPRESSION GENIQUE
Publication
Application
Priority
- US 2006046920 W 20061208
- US 75092905 P 20051216
- US 76205606 P 20060124
- US 63638506 A 20061207
Abstract (en)
[origin: WO2007078599A2] The present invention provides compositions, kits, assemblies, libraries, arrays, and high throughput methods for large scale structural and functional characterization of gene expression regulatory elements in a genome of an organism, especially in a human genome. In one aspect of the invention, an array of expression constructs is provided, e.ach of the expression constructs comprising: a nucleic acid segment operably linked with a reporter sequence in an expression vector such that expression of the reporter sequence is under the transcriptional control of the nucleic acid segment, the nucleic acid segment varying in the library and having a diversity of at least 50. The nucleic acid segments can be a large library of gene expression regulatory elements such as transcriptional promoters. The present invention can have a wide variety of applications such as in personalized medicine, pharmacogenomics, and correlation of polymorphisms with phenotypic traits.
IPC 8 full level
CPC (source: EP)
C12N 15/1086 (2013.01); C12Q 1/6897 (2013.01)
Citation (search report)
- [X] US 6200760 B1 20010313 - DANNENBERG ANDREW J [US], et al
- [X] COOPER SARA J ET AL: "Comprehensive analysis of transcriptional promoter structure and function in 1% of the human genome.", GENOME RESEARCH JAN 2006, vol. 16, no. 1, 12 December 2005 (2005-12-12), pages 1 - 10, XP009126140, ISSN: 1088-9051
- [XI] TRINKLEIN NATHAN D ET AL: "An abundance of bidirectional promoters in the human genome.", GENOME RESEARCH, vol. 14, no. 1, January 2004 (2004-01-01), pages 62 - 66, XP009126141, ISSN: 1088-9051
- [A] TRINKLEIN NATHAN D ET AL: "Identification and functional analysis of human transcriptional promoters.", GENOME RESEARCH FEB 2003, vol. 13, no. 2, February 2003 (2003-02-01), pages 308 - 312, XP009126139, ISSN: 1088-9051
- [A] KIM TAE HOON ET AL: "Direct isolation and identification of promoters in the human genome.", GENOME RESEARCH JUN 2005, vol. 15, no. 6, June 2005 (2005-06-01), pages 830 - 839, XP002412922, ISSN: 1088-9051
- [A] ENCODE PROJECT CONSORTIUM: "The ENCODE (ENCyclopedia Of DNA Elements) Project.", SCIENCE (NEW YORK, N.Y.) 22 OCT 2004, vol. 306, no. 5696, 22 October 2004 (2004-10-22), pages 636 - 640, XP009126138, ISSN: 1095-9203
- [A] WILSON V S ET AL: "A NOVEL CELL LINE, MDA-KB2, THAT STABLY EXPRESSES AN ANDROGEN- AND GLUCOCORTICOID-RESPONSIVE REPORTER FOR THE DETECTION OF HORMONE RECEPTOR AGONISTS AND ANTAGONISTS", TOXICOLOGICAL SCIENCES, ACADEMIC PRESS, SAN DIEGO, FL, US, vol. 66, no. 1, 1 March 2002 (2002-03-01), pages 69 - 81, XP009028294, ISSN: 1096-6080
- See references of WO 2007078599A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2007078599 A2 20070712; WO 2007078599 A3 20080828; WO 2007078599 A8 20081030; WO 2007078599 A9 20071004; EP 2021499 A2 20090211; EP 2021499 A4 20100217; JP 2009519710 A 20090521
DOCDB simple family (application)
US 2006046920 W 20061208; EP 06849046 A 20061208; JP 2008545677 A 20061208