EP 2032984 A1 20090311 - MICROFLUIDIC ASSAYS AND MICROFLUIDIC DEVICES
Title (en)
MICROFLUIDIC ASSAYS AND MICROFLUIDIC DEVICES
Title (de)
MIKROFLUID-TESTVERFAHREN UND -VORRICHTUNGEN
Title (fr)
DOSAGES MICROFLUIDIQUES ET DISPOSITIFS MICROFLUIDIQUES
Publication
Application
Priority
SE 2005001887 W 20051212
Abstract (en)
[origin: WO2007069940A1] The invention is a method for determining in the amount of an analyte (An) in a sample. The method comprises competitive immunoassays and enzymatic assays in which a soluble product (immune complex and enzymatic product, respectively) is formed. The product is subsequently measured in a measuring zone of a microchannel structure of a microfluidic device. The characteristic features are: A) Competitive assays utilize: a) an An-analogue that exhibits an immobilizing tag, and an anti-An that exhibits a detectable group I, or b) an An-analogue that is a conjugate between an analyte moiety and a label which are bound together via a bridge that preferably is hydrophilic and/or polymeric, and anti-An exhibits an immobilizing tag, and B) Catalytic assays utilize a substrate that: a) exhibits an immobilizing tag, or b) is devoid of an immobilizing tag but contains a group that is transformable to such a tag, C) P is obtained in dissolved form and exhibits the immobilizing tag and the detectable group I, D) MZ contains predisposed solid phase, and B) step (ii) comprises the substeps of: a) immobilizing P selectively to the solid phase, and b) measuring the amount of immobilized P.
IPC 8 full level
G01N 33/543 (2006.01); B01J 19/00 (2006.01); B01L 3/00 (2006.01); G01N 35/00 (2006.01)
CPC (source: EP)
B01L 3/5027 (2013.01); C12Q 1/00 (2013.01); G01N 33/54366 (2013.01); B01L 2200/0605 (2013.01); B01L 2300/0806 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/0867 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0688 (2013.01)
Citation (search report)
See references of WO 2007069940A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA HR MK YU
DOCDB simple family (publication)
WO 2007069940 A1 20070621; EP 2032984 A1 20090311; EP 2237037 A1 20101006
DOCDB simple family (application)
SE 2005001887 W 20051212; EP 05816369 A 20051212; EP 10169709 A 20051212