EP 2049659 A1 20090422 - VECTOR CONSTRUCTS AND METHODS OF EXPRESSING AND SECRETING POLYPEPTIDES IN FILAMENTOUS FUNGI USING A SELF-PROCESSING 2A CLEAVAGE SITE
Title (en)
VECTOR CONSTRUCTS AND METHODS OF EXPRESSING AND SECRETING POLYPEPTIDES IN FILAMENTOUS FUNGI USING A SELF-PROCESSING 2A CLEAVAGE SITE
Title (de)
VEKTORKONSTRUKTE UND VERFAHREN ZUR EXPRESSION UND SEKRETION VON POLYPEPTIDEN IN FILAMENTÖSEN PILZEN UNTER VERWENDUNG EINER SELBSTPROZESSIERENDEN 2A-SPALTSTELLE
Title (fr)
ASSEMBLAGES DE VECTEURS ET PROCEDE D'EXPRESSION ET DE SECRETION DE POLYPEPTIDES DANS DES CHAMPIGNONS FILAMENTEUX EN UTILISANT UN SITE DE COUPURE 2A A AUTOMATURATION
Publication
Application
Priority
- EP 2007006448 W 20070719
- DE 102006034124 A 20060724
- DE 102006052204 A 20061106
Abstract (en)
[origin: WO2008012028A1] The invention relates to a vector construct for expressing and secreting polypeptides in filamentous fungi, comprising, in 5'3' direction, in functional linkage, - optionally a promoter which may have an enhancer placed upstream, at least one DNA sequence which codes for a first polypeptide and which may comprise a signal sequence or not, - optionally a DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, - a DNA sequence which codes for a second polypeptide with signal sequence, optionally a further DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, - optionally a DNA sequence which codes for a third polypeptide with signal sequence, optionally a further DNA sequence which codes for a 2A cleavage site or for a sequence derived therefrom, optionally together with an upstream DNA sequence which codes for a proteolytic cleavage site, or optionally a DNA sequence which codes for a proteolytic cleavage site, optionally a DNA sequence which codes for a fourth polypeptide with signal sequence, and a terminator, where at least one 2A cleavage site or a sequence derived therefrom is present in the construct.
IPC 8 full level
C12N 9/64 (2006.01)
CPC (source: EP US)
C12N 15/80 (2013.01 - EP US); C12P 21/06 (2013.01 - EP US); C07K 2319/50 (2013.01 - EP US)
Citation (search report)
See references of WO 2008012028A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA HR MK RS
DOCDB simple family (publication)
WO 2008012028 A1 20080131; EP 2049659 A1 20090422; US 2010178671 A1 20100715
DOCDB simple family (application)
EP 2007006448 W 20070719; EP 07765236 A 20070719; US 30936507 A 20070719