EP 2054530 A1 20090506 - DUAL FUNCTION PRIMERS FOR AMPLIFYING DNA AND METHODS OF USE

Title (en)

DUAL FUNCTION PRIMERS FOR AMPLIFYING DNA AND METHODS OF USE

Title (de)

PRIMER MIT DOPPELFUNKTION ZUR DNA-VERVIELFALTIGUNG UND VERWENDUNGSVERFAHREN DAFÜR

Title (fr)

AMORCES BIFONCTIONNELLES POUR AMPLIFIER DE L'ADN ET LEURS PROCÉDÉS D'UTILISATION

Publication

EP 2054530 A1 20090506 (EN)

Application

EP 06846404 A 20061130

Priority

US 2006061366 W 20061130
US 56307206 A 20061124

Abstract (en)

[origin: US2009068643A1] The present invention provides novel nucleotide compositions that enable the detection of DNA synthesis products and methods for use thereof. In one embodiment, the method can be used in PCR and allows the progress of the reaction to be monitored as it occurs. In one embodiment, the invention employs at least one fluorescence-quenched oligonucleotide that can prime DNA extension reactions. In a second embodiment, the invention employs at least one fluorescence-quenched oligonucleotide that can function as a template for DNA extension reactions. In both embodiments, the oligonucleotide also functions as a probe for detecting the progress of successive extension reaction cycles. Signal detection is dependent upon DNA synthesis and can occur with or without probe cleavage.

IPC 8 full level

C12Q 1/68 (2006.01); C12P 19/34 (2006.01)

CPC (source: EP US)

C12Q 1/6823 (2013.01 - EP US); C12Q 1/6853 (2013.01 - EP US); C12Q 1/686 (2013.01 - EP US)

C-Set (source: EP US)

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

Designated extension state (EPC)

AL BA HR MK RS

DOCDB simple family (publication)

US 2009068643 A1 20090312; AU 2006350989 A1 20080529; CA 2670845 A1 20080529; EP 2054530 A1 20090506; EP 2054530 A4 20091111; JP 2010510778 A 20100408; JP 5203381 B2 20130605; WO 2008063194 A1 20080529

DOCDB simple family (application)

US 56307206 A 20061124; AU 2006350989 A 20061130; CA 2670845 A 20061130; EP 06846404 A 20061130; JP 2009538380 A 20061130; US 2006061366 W 20061130