EP 2059906 A2 20090520 - METHOD FOR THE QUANTITATIVE DETERMINATION OF THE COLOCALIZATION OF MOLECULAR MARKERS IN TISSUE SECTIONS
Title (en)
METHOD FOR THE QUANTITATIVE DETERMINATION OF THE COLOCALIZATION OF MOLECULAR MARKERS IN TISSUE SECTIONS
Title (de)
VERFAHREN ZUR QUANTITATIVEN BESTIMMUNG DER KOLOKALISATION VON MOLEKÜLMARKERN IN GEWEBESCHNITTEN
Title (fr)
PROCÉDÉ DE DÉTERMINATION QUANTITATIVE DE LA CO-LOCALISATION DE MARQUEURS MOLÉCULAIRES DANS LES COUPES DE TISSU
Publication
Application
Priority
- EP 2007058280 W 20070809
- DE 102006040513 A 20060830
Abstract (en)
[origin: WO2008025662A2] The invention relates to a method for the quantitative determination of the colocalization of at least two molecular markers in tissue sections, especially in sections of cutaneous and mucosal tissue. The method of the invention is characterized by the following procedural steps: generation of a digital image of the tissue section for each of the at least two molecular markers for visualization of the respective molecular marker, superimposition of the digital images to give a summed image, pixelwise quantization of the molecular marker signal for each of the at least two molecular markers in the summed image, generation of a matrix, where a vector whose components are the quantized molecular marker signals of the at least two molecular markers is assigned to each pixel, and tissue-specific standardized alignment of the matrix. The invention further relates to an apparatus for the quantitative determination of the colocalization of at least two molecular markers in tissue sections, especially in sections of cutaneous and mucosal tissue, and to the use of the method of the invention.
IPC 8 full level
G06T 7/00 (2006.01)
CPC (source: EP)
G01N 33/5091 (2013.01); G06T 7/00 (2013.01)
Citation (search report)
See references of WO 2008025662A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA HR MK RS
DOCDB simple family (publication)
WO 2008025662 A2 20080306; WO 2008025662 A3 20080724; DE 102006040513 A1 20080327; EP 2059906 A2 20090520
DOCDB simple family (application)
EP 2007058280 W 20070809; DE 102006040513 A 20060830; EP 07788341 A 20070809