EP 2061891 B1 20120411 - EXPRESSION IN INSECT CELLS OF GENES WITH OVERLAPPING OPEN READING FRAMES, METHODS AND COMPOSITIONS THEREFOR
Title (en)
EXPRESSION IN INSECT CELLS OF GENES WITH OVERLAPPING OPEN READING FRAMES, METHODS AND COMPOSITIONS THEREFOR
Title (de)
EXPRESSION VON GENEN MIT ÜBERLAPPENDEN OFFENEN LESERASTERN IN INSEKTENZELLEN, VERFAHREN UND ZUSAMMENSETZUNGEN DAFÜR
Title (fr)
EXPRESSION DANS DES CELLULES D'INSECTE DE GÈNES AYANT DES CADRES OUVERTS DE LECTURE CHEVAUCHANTS, PROCÉDÉS ET COMPOSITIONS POUR CELA
Publication
Application
Priority
- US 2007076799 W 20070824
- US 83976106 P 20060824
Abstract (en)
[origin: WO2008024998A2] The present teachings disclose nucleic acid cassettes for expressing in an insect cell a plurality of polypeptides encoded by a gene comprising overlapping open reading frames (ORFs). A cassette comprises, in 5' to 3' order, a) a first insect cell-operabie promoter, b) a 5' portion of a gene comprising a first ORF of the gene, c) an intron comprising a second insect cell-operable promoter, and d) a 3' portion of the gene comprising at least one additional ORF. Vectors and insect cells comprising the cassettes are also disclosed, as well as methods for production of recombinant adeno- associated virus in insect cells using the cassettes.
IPC 8 full level
C12N 15/86 (2006.01); C12N 5/00 (2006.01); C12N 15/00 (2006.01)
CPC (source: EP US)
C07K 14/005 (2013.01 - US); C12N 7/00 (2013.01 - EP US); C12N 15/85 (2013.01 - EP US); C12N 15/86 (2013.01 - US); C12N 2750/00043 (2013.01 - US); C12N 2750/00051 (2013.01 - US); C12N 2750/14151 (2013.01 - EP US); C12N 2750/14152 (2013.01 - US); C12N 2800/105 (2013.01 - EP US); C12N 2800/50 (2013.01 - EP US); C12N 2830/42 (2013.01 - US); C12N 2840/20 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2008024998 A2 20080228; WO 2008024998 A3 20081106; AT E553206 T1 20120415; CN 101522903 A 20090902; CN 101522903 B 20130731; DK 2061891 T3 20120723; EP 2061891 A2 20090527; EP 2061891 A4 20100728; EP 2061891 B1 20120411; ES 2385679 T3 20120730; US 2009203071 A1 20090813; US 2014329313 A1 20141106; US 2015031115 A1 20150129; US 2016333375 A1 20161117; US 8945918 B2 20150203; US 9879279 B2 20180130; US 9879282 B2 20180130
DOCDB simple family (application)
US 2007076799 W 20070824; AT 07814439 T 20070824; CN 200780037031 A 20070824; DK 07814439 T 20070824; EP 07814439 A 20070824; ES 07814439 T 20070824; US 201414297165 A 20140605; US 201414505847 A 20141003; US 201615224984 A 20160801; US 29795807 A 20070824