EP 2084285 A4 20100113 - CO-EXPRESSION OF PROLINE HYDROXYLASES TO FACILITATE HYP-GLYCOSYLATION OF PROTEINS EXPRESSED AND SECRETED IN PLANT CELLS
Title (en)
CO-EXPRESSION OF PROLINE HYDROXYLASES TO FACILITATE HYP-GLYCOSYLATION OF PROTEINS EXPRESSED AND SECRETED IN PLANT CELLS
Title (de)
COEXPRESSION VON PROLIN-HYDROXYLASEN ZUR ERLEICHTERUNG DER HYP-GLYKOSYLIERUNG VON IN PFLANZENZELLEN EXPRIMIERTEN UND SEZERNIERTEN PROTEINEN
Title (fr)
CO-EXPRESSION DE PROLINES HYDROXYLASES AFIN DE FACILITER L'HYP-GLYCOSYLATION DE PROTÉINES EXPRIMÉES ET SECRÉTÉES DANS DES CELLULES VÉGÉTALES
Publication
Application
Priority
- US 2007073137 W 20070710
- US 81955706 P 20060710
Abstract (en)
[origin: WO2008008766A2] Plant cells contain particular proline hydroxylases whose specificity determines which prolines, in a protein expressed therein, are hydroxylated. The resulting hydroxyprolines, in turn, my be glycosylated. It is the presence of glycosylated hydroxyprolines which is the most important determinant of the degree of secretion of the protein. One may modify the proline hydroxylation capabilities of the plant cell by co-expressing in that cell at least one exogeneous proline hyrdoxylase which will hydroxylate at least one proline site in the protein which is not hydroxylated by the endogeneous proline hydroxylase, but which, if hydroxylated, can than be glycosylated by the plant cell, resulting in increased Hyp-glycosylation. Moreover, even if the co-expressed exogenous proline hydroxylase doesn't achieve the desired level of Hyp-glycosylation in the native protein, such co-expression may still be used in conjunction with modification of the protein itself to increase the number and/or the likelihood of Hyp-glycosylation sites.
IPC 8 full level
C12N 15/82 (2006.01)
CPC (source: EP)
C07K 14/78 (2013.01); C12N 9/0071 (2013.01); C12N 15/8257 (2013.01); C12P 21/005 (2013.01)
Citation (search report)
- [X] WO 2006035442 A2 20060406 - COLLPLANT LTD [IL], et al
- [DX] MERLE C ET AL: "Hydroxylated human homotrimeric collagen I in Agrobacterium tumefaciens-mediated transient expression and in transgenic tobacco plant", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 515, no. 1-3, 27 March 2002 (2002-03-27), pages 114 - 118, XP004347752, ISSN: 0014-5793
- [DA] BERRA EDURNE ET AL: "The hypoxia-inducible-factor hydroxylases bring fresh air into hypoxia signalling", EMBO REPORTS, vol. 7, no. 1, January 2006 (2006-01-01), pages 41 - 45, XP002558270, ISSN: 1469-221X
- See references of WO 2008008766A2
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2008008766 A2 20080117; WO 2008008766 A3 20081120; EP 2084285 A2 20090805; EP 2084285 A4 20100113
DOCDB simple family (application)
US 2007073137 W 20070710; EP 07799434 A 20070710