Title (en)

METHOD, KIT AND INITIATORS FOR GENETIC IDENTIFICATION OF MYCOBACTERIUM BOVIS BCG VARIETY MOREAU-RJ, METHOD FOR MONITORING FEASIBILITY OF BCG MOREAU-RJ AND EXPRESSION OF GENES, AND PRODUCTS WITH IMMUNOLOGICAL ACTIVITY

Title (de)

VERFAHREN, KIT UND INITIATOREN ZUR GENETISCHEN IDENTIFIZIERUNG DER MYCOBACTERIUM BOVIS BCG-VARIETÄT MOREAU-RJ, VERFAHREN ZUR ÜBERPRÜFUNG DER EIGNUNG VON BCG MOREAU-RJ UND EXPRESSION VON GENEN SOWIE PRODUKTE MIT IMMUNOLOGISCHER AKTIVITÄT

Title (fr)

PROCÉDÉ, TROUSSE ET INITIATEURS D'IDENTIFICATION GÉNÉTIQUE DE MYCOBACTERIUM BOVIS BCG DE TYPE MOREAU-RJ, PROCÉDÉ DE CONTRÔLE DE POSSIBILITÉ D'OBTENTION DE BCG MOREAU-RJ ET D'EXPRESSION DE GÈNES ET PRODUITS À ACTIVITÉ IMMUNOLOGIQUE

Publication

EP 2097522 A4 20100210 (EN)

Application

EP 07815763 A 20071123

Priority

• BR 2007000322 W 20071123
• BR PI0604958 A 20061123

Abstract (en)

[origin: WO2008061331A2] This invention refers to the mapping of insertions, deletions and point mutations of BCG Moreau from determining the genomic sequence of BCG Moreau and his comparison with the genome of other mycobacteriae. Moreover, this invention identifies nucleotidic and peptidic sequences, which provide methodologies for genetic verification of BCG Moreau, and for the distinction between BCG Moreau and other mycobacteriae, including in biological and clinical samples. The present invention supplies, furthermore, methods and kits for identification of such sequences, of the products of expression of these sequences or antibodies generated from this expression. The invention furthermore provides methodologies for monitoring and preparation of vaccines and kits for verifying the gene transcription/expression of certain antigens of the bacillus. From this invention are also provided secondary products with immunomodulatory activity, for example, in the treatment of bladder cancer and asthma.

IPC 8 full level

C12N 15/09 (2006.01); C12R 1/32 (2006.01)

CPC (source: EP)

A61P 35/00 (2017.12); C07K 14/35 (2013.01); C12N 1/205 (2021.05); C12Q 1/689 (2013.01); G01N 33/5695 (2013.01); A61K 38/00 (2013.01); C12R 2001/32 (2021.05)

Citation (search report)

• [X] TSOLAKI ANTHONY G ET AL: "Genomic deletions classify the Beijing/W strains as a distinct genetic lineage of Mycobacterium tuberculosis.", JOURNAL OF CLINICAL MICROBIOLOGY JUL 2005, vol. 43, no. 7, July 2005 (2005-07-01), pages 3185 - 3191, XP002558329, ISSN: 0095-1137
• [X] KONG Y ET AL: "Population-based study of deletions in five different genomic regions of Mycobacterium tuberculosis and possible clinical relevance of the deletions.", JOURNAL OF CLINICAL MICROBIOLOGY NOV 2006, vol. 44, no. 11, 6 September 2006 (2006-09-06), pages 3940 - 3946, XP002558330, ISSN: 0095-1137
• [A] BROSCH ROLAND ET AL: "Comparative genomics uncovers large tandem chromosomal duplications in Mycobacterium bovis BCG pasteur", YEAST, vol. 17, no. 2, 30 June 2000 (2000-06-30), pages 111 - 123, XP008115720, ISSN: 0749-503X
• [A] BEHR M A ET AL: "Comparative genomics of BCG vaccines by whole-genome DNA microarray", SCIENCE (WASHINGTON D C), vol. 284, no. 5419, 28 May 1999 (1999-05-28), pages 1520 - 1523, XP002558331, ISSN: 0036-8075
• [A] GARNIER T ET AL: "THE COMPLETE GENOME SEQUENCE OF MYCOBACTERIUM BOVIS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 100, no. 13, 24 June 2003 (2003-06-24), pages 7877 - 7882, XP001205229, ISSN: 0027-8424
• See references of WO 2008061331A2

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

DOCDB simple family (publication)

WO 2008061331 A2 20080529; WO 2008061331 A3 20081106; BR PI0604958 A 20080708; BR PI0604958 B1 20220517; EP 2097522 A2 20090909; EP 2097522 A4 20100210

DOCDB simple family (application)

BR 2007000322 W 20071123; BR PI0604958 A 20061123; EP 07815763 A 20071123