EP 2103925 A2 20090923 - Multi-exposure imaging for automated fluorescent microscope slide scanning
Title (en)
Multi-exposure imaging for automated fluorescent microscope slide scanning
Title (de)
Bildgebung mit mehrfacher Belichtung zur automatischen Fluoreszenzmikroskop-Objektträgerabtastung
Title (fr)
Imagerie multi-exposition pour balayage automatique de lames pour microscope à fluorescence
Publication
Application
Priority
US 5351508 A 20080321
Abstract (en)
The present invention provides methods and systems that acquire digital images of a microscope slide having a large variation in pixel value brightness, like, for instance, fluorescent microscope slides. The methods and the systems generate a well contrasted composite image with preserved areas of low and high fluorescent intensity from the input images. One method includes: acquiring an image of a fluorescent microscope slide at a first exposure level resulting in a first acquired image at a first contrast level; acquiring additional images of the fluorescent microscope slide each at an exposure level different from each other and different from the first exposure, resulting in different acquired images at different contrast levels; and forming a composite image of the fluorescent microscope slide by forming a weighted sum of the intensity values of the corresponding pixels of each of the images, whereby composite image has a composite contrast level over the entire composite image that represents a weighted average of the contrast levels.
IPC 8 full level
G01N 21/64 (2006.01); G06T 5/50 (2006.01)
CPC (source: EP US)
G01N 21/6458 (2013.01 - EP US); G06T 5/50 (2013.01 - EP US); G06T 2207/20221 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA RS
DOCDB simple family (publication)
EP 2103925 A2 20090923; EP 2103925 A3 20130220; EP 2103925 B1 20190904; US 2009238435 A1 20090924
DOCDB simple family (application)
EP 09250636 A 20090305; US 5351508 A 20080321