EP 2118305 A2 20091118 - METHODS AND KITS FOR AMPLIFYING DNA
Title (en)
METHODS AND KITS FOR AMPLIFYING DNA
Title (de)
VERFAHREN UND KITS ZUR DNA-AMPLIFIKATION
Title (fr)
PROCÉDÉS ET KITS POUR AMPLIFIER L'ADN
Publication
Application
Priority
US 2007063103 W 20070301
Abstract (en)
[origin: WO2008108843A2] Novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic are disclosed (/. e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, methods of nucleic acid amplification are disclosed which are robust and efficient, while reducing the appearance of side-products. In general, the methods use priming oligonucleotides that target only one sense of a target nucleic acid, a promoter oligonucleotide modified to prevent polymerase extension from its 3 '-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-products. The disclosed methods minimizes or substantially eliminate the emergence of side-products, thus providing ahigh level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The disclosed methods minimize or substantially eliminate this problem, thus providing enhanced levels of sensitivity.
IPC 8 full level
C07H 21/02 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/6865 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2008108843 A2 20080912; WO 2008108843 A3 20081030; AU 2007348215 A1 20080912; CA 2678799 A1 20080912; EP 2118305 A2 20091118; EP 2118305 A4 20101110; JP 2010520750 A 20100617
DOCDB simple family (application)
US 2007063103 W 20070301; AU 2007348215 A 20070301; CA 2678799 A 20070301; EP 07757746 A 20070301; JP 2009551981 A 20070301