EP 2126100 B1 20121114 - IMPROVED METHOD FOR HOMOLOGOUS RECOMBINATION
Title (en)
IMPROVED METHOD FOR HOMOLOGOUS RECOMBINATION
Title (de)
VERBESSERTES VERFAHREN ZUR HOMOLOGEN REKOMBINATION
Title (fr)
PROCÉDÉ AMÉLIORÉ DE RECOMBINAISON ALLOGÉNIQUE
Publication
Application
Priority
- EP 2008053352 W 20080320
- EP 07104588 A 20070321
- EP 07111826 A 20070705
- EP 08735442 A 20080320
Abstract (en)
[origin: WO2008113847A2] The present invention discloses a method to construct eukaryotic cells having a target sequence in a chromosomal DNA sequence replaced by a replacement sequence of interest comprising: - modifying a parent eukaryotic cell with a preference for NHR to provide a eukaryotic cell having an increased HR/NHR ratio as compared to the parent cell, - providing two sets of DNA molecules of which the first set comprises DNA molecules each comprising a first non-functional fragment of the replacement sequence of interest flanked at its 5'-side by a DNA sequence substantially homologous to a sequence of the chromosomal DNA flanking the target sequence and the second set comprises DNA molecules each comprising a second non-functional fragment of the DNA replacement sequence of interest overlapping with the first non-functional fragment and flanked at its 3'-side by a DNA sequence substantially homologous to a sequence of the chromosomal DNA flanking the target sequence, wherein the first and second non-functional fragments become functional upon recombination; - transforming the modified eukaryotic cells with both sets of DNA molecules; and - growing the cells to obtain transformed progeny cells having a DNA molecule from the first set recombined with a DNA molecule from the second set into a DNA molecule comprising a functional replacement sequence and having the target sequence replaced by the functional replacement sequence.
IPC 8 full level
C12N 15/90 (2006.01)
CPC (source: EP US)
C07K 14/385 (2013.01 - EP US); C12N 15/1058 (2013.01 - EP US); C12N 15/80 (2013.01 - US); C12N 15/902 (2013.01 - EP US); C12N 2800/30 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
DOCDB simple family (publication)
WO 2008113847 A2 20080925; WO 2008113847 A3 20090212; DK 2126100 T3 20130114; EP 2126100 A2 20091202; EP 2126100 B1 20121114; EP 2592149 A1 20130515; US 2010120154 A1 20100513; US 2014154807 A1 20140605
DOCDB simple family (application)
EP 2008053352 W 20080320; DK 08735442 T 20080320; EP 08735442 A 20080320; EP 12182937 A 20080320; US 201414146858 A 20140103; US 53220508 A 20080320