EP 2126134 A1 20091202 - METHOD AND TEST KIT FOR THE RAPID DETECTION OF SPECIFIC NUCLEIC ACID SEQUENCES, ESPECIALLY FOR DETECTING MUTATIONS OR SNPS
Title (en)
METHOD AND TEST KIT FOR THE RAPID DETECTION OF SPECIFIC NUCLEIC ACID SEQUENCES, ESPECIALLY FOR DETECTING MUTATIONS OR SNPS
Title (de)
VERFAHREN UND TESTKIT ZUM SCHNELLEN NACHWEIS SPEZIFISCHER NUKLEINSÄURESEQUENZEN, INSBESONDERE ZUM NACHWEIS VON MUTATIONEN ODER SNP'S
Title (fr)
PROCEDE ET KIT DE TEST PERMETTANT LA DETECTION RAPIDE DE SEQUENCES D'ACIDES NUCLEIQUES SPECIFIQUES, NOTAMMENT LA DETECTION DE MUTATIONS OU DE POLYMORPHISMES D'UN SEUL NUCLEOTIDE (SNP)
Publication
Application
Priority
- EP 2008053065 W 20080314
- DE 102007013099 A 20070314
Abstract (en)
[origin: WO2008110622A1] The invention relates to a method and a test kit for the rapid detection of specific nucleic acid sequences, especially for the detection of mutations or SNPs (SNP = single nucleotide polymorphism), the detection reaction taking place in two steps. In a first step, the target-specific amplification reaction is carried out, together with the probe hybridising reaction using fluorescence-marked allele-specific amplification primers. In the second step, the fluorescence is detected by means of commercial fluorescence readers. The genotyping is carried out on the basis of the quotients of the end point fluorescence of the samples and the negative controls.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6858 (2013.01 - EP US)
Citation (search report)
See references of WO 2008110622A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
DOCDB simple family (publication)
DE 102007013099 A1 20080918; EP 2126134 A1 20091202; US 2010099099 A1 20100422; WO 2008110622 A1 20080918
DOCDB simple family (application)
DE 102007013099 A 20070314; EP 08735420 A 20080314; EP 2008053065 W 20080314; US 55925209 A 20090914