Global Patent Index - EP 2152853 A1

EP 2152853 A1 20100217 - IN VITRO MODEL OF LATENT MYCOBACTERIAL INFECTION

Title (en)

IN VITRO MODEL OF LATENT MYCOBACTERIAL INFECTION

Title (de)

IN-VITRO-MODELL EINER LATENTEN MYKOBAKTERIENINFEKTION

Title (fr)

MODÈLE IN VITRO D'INFECTION MYCOBACTÉRIENNE LATENTE

Publication

EP 2152853 A1 20100217 (EN)

Application

EP 08746514 A 20080422

Priority

  • US 2008061110 W 20080422
  • US 91483807 P 20070430

Abstract (en)

[origin: WO2008134298A1] A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing it to multiple stress conditions, including a low nutrient culture medium without glycerol, a low pH, a relatively high level of carbon dioxide and a relatively low gas phase oxygen level. An in vitro model of mycobacterial infection employs macrophages induced from THP1 cells which are then infected with Mycobacterium. The infected macrophages are grown under hypoxic conditions to induce latency in the mycobacteria. The in vitro model of infection is useful in evaluating compounds for activity against latent mycobacteria.

IPC 8 full level

C12N 1/12 (2006.01); C12N 1/20 (2006.01)

CPC (source: EP US)

C12N 1/20 (2013.01 - EP US); C12N 1/36 (2013.01 - EP US)

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

Designated extension state (EPC)

AL BA MK RS

DOCDB simple family (publication)

WO 2008134298 A1 20081106; AU 2008245901 A1 20081106; AU 2008245901 B2 20141016; CA 2685746 A1 20081103; CA 2685746 C 20170228; EP 2152853 A1 20100217; EP 2152853 A4 20100616; US 2009023596 A1 20090122; US 2011033914 A1 20110210; US 2011033915 A1 20110210; US 2013040829 A1 20130214; US 8012492 B2 20110906; US 8211446 B2 20120703; US 8241644 B2 20120814; US 8551499 B2 20131008

DOCDB simple family (application)

US 2008061110 W 20080422; AU 2008245901 A 20080422; CA 2685746 A 20080422; EP 08746514 A 20080422; US 10714608 A 20080422; US 201213572888 A 20120813; US 87094510 A 20100830; US 87095210 A 20100830