Global Patent Index - EP 2154159 A4

EP 2154159 A4 20120321 - ACTIVATED PROTEASE INDICATOR

Title (en)

ACTIVATED PROTEASE INDICATOR

Title (de)

INDIKATOR FÜR AKTIVIERTE PROTEASE

Title (fr)

INDICATEUR DE PROTÉASE ACTIVÉE

Publication

EP 2154159 A4 20120321 (EN)

Application

EP 08752552 A 20080509

Priority

  • JP 2008058669 W 20080509
  • JP 2007124924 A 20070509

Abstract (en)

[origin: EP2154159A1] It is an object of the invention to provide novel approaches capable of detecting activated protease and also detecting protease activation on real time at a high sensitivity in a noninvasive manner. By the method for detecting activated protease of the invention, an indicator in a circular form comprising the C-half fragment of luciferase (Luc-C) and the N-half fragment of luciferase (Luc-N) linked together through a substrate peptide for a protease is introduced in an in vitro assay system or in cells. Upon digestion of the substrate peptide by the protease, Luc-N and Luc-C together reconstructs active luciferase, so that the activated protease can be detected by assaying the luminescence signal from the luciferase.

IPC 8 full level

C07K 19/00 (2006.01); C12N 9/02 (2006.01); C12N 15/09 (2006.01); C12Q 1/37 (2006.01); C12Q 1/66 (2006.01)

CPC (source: EP US)

C12N 9/0069 (2013.01 - EP US); C12Q 1/37 (2013.01 - EP US); C12Q 1/66 (2013.01 - EP US); C07K 2319/50 (2013.01 - EP US); G01N 2500/04 (2013.01 - EP US)

Citation (search report)

  • [I] WO 2005042034 A1 20050512 - UNIV CALIFORNIA [US], et al
  • [A] WO 0206458 A2 20020124 - CHEMICON INTERNATIONAL INC [US]
  • [XP] WO 2007120522 A2 20071025 - PROMEGA CORP [US], et al
  • [X] WO 2005038029 A2 20050428 - PROMEGA CORP [US], et al
  • [X] US 2005153310 A1 20050714 - FAN FRANK [US], et al
  • [A] OZAWA T ET AL: "SPLIT LUCIFERASE AS AN OPTICAL PROBE FOR DETECTING PROTEIN-PROTEIN INTERACTIONS IN MAMMALIAN CELLS BASED ON PROTEIN SPLICING", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 73, no. 11, 1 June 2001 (2001-06-01), pages 2516 - 2521, XP001081130, ISSN: 0003-2700, DOI: 10.1021/AC0013296
  • [XP] FAN FRANK ET AL: "Novel genetically encoded biosensors using firefly luciferase", ACS CHEMICAL BIOLOGY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 3, no. 6, 20 June 2008 (2008-06-20), pages 346 - 351, XP002553151, ISSN: 1554-8929, [retrieved on 20080620], DOI: 10.1021/CB8000414
  • [XP] KANNO A ET AL: "Cyclic luciferase for real-time sensing for caspase-3 activities in living mammals", ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, WILEY VCH VERLAG, WEINHEIM, vol. 46, no. 40, 8 October 2007 (2007-10-08), pages 7595 - 7599, XP008123060, ISSN: 1433-7851, [retrieved on 20070827], DOI: 10.1002/ANIE.200700538
  • See references of WO 2008140060A1

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

DOCDB simple family (publication)

EP 2154159 A1 20100217; EP 2154159 A4 20120321; JP 4491516 B2 20100630; JP WO2008140060 A1 20100805; US 2010297620 A1 20101125; US 8658777 B2 20140225; WO 2008140060 A1 20081120

DOCDB simple family (application)

EP 08752552 A 20080509; JP 2008058669 W 20080509; JP 2008548903 A 20080509; US 45134108 A 20080509