EP 2160463 A1 20100310 - A METHOD FOR THE CONSTRUCTION OF CANCER-SPECIFIC PROMOTERS USING FUNCTIONAL GENOMICS
Title (en)
A METHOD FOR THE CONSTRUCTION OF CANCER-SPECIFIC PROMOTERS USING FUNCTIONAL GENOMICS
Title (de)
VERFAHREN ZUR KONSTRUKTION KREBSSPEZIFISCHER PROMOTOREN UNTER VERWENDUNG FUNKTIONELLER GENOMIK
Title (fr)
PROCÉDÉ DESTINÉ À CONSTRUIRE DES PROMOTEURS SPÉCIFIQUES DU CANCER UTILISANT LA GÉNOMIQUE FONCTIONNELLE
Publication
Application
Priority
GR 2007000016 W 20070305
Abstract (en)
[origin: WO2008107725A1] The present invention relates to a method of producing a promoter element capable of regulating gene expression in a cell or tissue type of interest, said method comprising: (g) providing a plurality of transcription factor regulatory elements that are associated with genes that are expressed in said cell or tissue type; (h) constructing a library of expression vectors, wherein each vector comprises two or more of said transcription factor regulatory elements of (a) and a minimal promoter operably linked to an antibiotic resistance gene; (i) transfecting cells of the cell or tissue type of interest with vectors from said library (b); (j) culturing said cells under conditions suitable for gene expression and screening said cells for antibiotic resistance; and (k) identifying vectors in which the antibiotic resistance gene is expressed, thereby identifying a promoter element capable of regulating gene expression in the cell or tissue type of interest.
IPC 8 full level
C12N 15/10 (2006.01)
CPC (source: EP)
C12N 15/1086 (2013.01); C12N 15/74 (2013.01); C12N 15/79 (2013.01)
Citation (search report)
See references of WO 2008107725A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA HR MK RS
DOCDB simple family (publication)
DOCDB simple family (application)
GR 2007000016 W 20070305; EP 07705396 A 20070305