EP 2206790 B1 20141126 - Method for reducing dispersion in nucleic acid amplification reaction
Title (en)
Method for reducing dispersion in nucleic acid amplification reaction
Title (de)
Verfahren zur Verringerung der Verteilung in einer Nukleinsäurenamplifikationsreaktion
Title (fr)
Procédé pour la réduction de la dispersion dans la réaction d'amplification d'acide nucléique
Publication
Application
Priority
JP 2009004431 A 20090113
Abstract (en)
[origin: EP2206790A1] It is an object of the present invention to provide a method for amplifying a nucleic acid, which does not require complicated temperature control and which can be carried out without using special enzyme or special primers. The present invention provides a method for amplifying a nucleic acid, which comprises the following steps (1) and (2): (1) a step of incubating a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase, at least two types of oligonucleotide primers, and a nucleic acid fragment acting as a template, at temperature (T1); and (2) a step of incubating the reaction solution at temperature (T2) that is higher than the temperature (T1) and is between 50°C or higher and 100°C or lower, following the step (1).
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6844 (2013.01 - EP US)
Citation (examination)
SHERIDAN G E C ET AL: "Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 64, no. 4, 1 April 1998 (1998-04-01), pages 1313 - 1318, XP002194990, ISSN: 0099-2240
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
DOCDB simple family (publication)
EP 2206790 A1 20100714; EP 2206790 B1 20141126; CN 101824449 A 20100908; JP 2010161935 A 20100729; US 2010184155 A1 20100722
DOCDB simple family (application)
EP 10150508 A 20100112; CN 201010003504 A 20100112; JP 2009004431 A 20090113; US 68608710 A 20100112