EP 2230317 A1 20100922 - Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms
Title (en)
Nucleic acid probe-based diagnostic assays for prokaryotic and eukaryotic organisms
Title (de)
Diagnosetests auf Basis einer Nukleinsäuresonde für prokaryotische und eukaryotische Organismen
Title (fr)
Réseaux de diagnostic à base de sondes d'acide nucléique pour organismes procaryotes et eucaryotes
Publication
Application
Priority
- EP 00925552 A 20000515
- IE 9900043 W 19990514
Abstract (en)
Use of the ssrA gene or tmRNA, an RNA transcript of the ssrA gene, or fragments thereof as target regions in a nucleic acid probe assay for the detection and identification of prokaryotic and/or eukaryotic organisms is described. Nucleotide sequence alignment of tmRNA sequences from various organisms can be used to identify regions of homology and non-homology within the sequences which in turn can be used to design both genus specific and species specific oligonucleotide probes. These newly identified regions of homology and non-homology provide the basis of identifying and detecting organisms at the molecular level. Oligonucleotide probes identified in this way can be used to detect tmRNA in samples thereby giving an indication of the viability of non-viral organisms present in various sample types.
IPC 8 full level
C12Q 1/68 (2006.01); G01N 33/50 (2006.01); A61K 31/7088 (2006.01); A61K 38/00 (2006.01); A61K 48/00 (2006.01); A61P 31/04 (2006.01); A61P 33/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/689 (2018.01); G01N 33/15 (2006.01); G01N 33/53 (2006.01); G01N 37/00 (2006.01)
CPC (source: EP US)
A61P 31/04 (2017.12 - EP); A61P 33/00 (2017.12 - EP); C12N 15/113 (2013.01 - US); C12Q 1/689 (2013.01 - EP US); C12Q 2600/158 (2013.01 - EP US)
Citation (applicant)
- EP 0395292 A2 19901031 - BARRY THOMAS GERARD [IE], et al
- KEILER, K.C. ET AL., SCIENCE, vol. 271, 1996, pages 990 - 993
- SHERIDAN, G.E.C. ET AL., APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 64, 1998, pages 1313 - 1318
Citation (search report)
- [A] US 5849497 A 19981215 - STEINMAN CHARLES [US]
- [A] EP 0630971 A2 19941228 - HOFFMANN LA ROCHE [CH]
- [A] WILLIAMS K P ET AL: "THE TMRNA WEBSITE", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB LNKD- DOI:10.1093/NAR/26.1.163, vol. 26, no. 1, 1 January 1998 (1998-01-01), pages 163 - 165, XP000874156, ISSN: 0305-1048
Designated contracting state (EPC)
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
Designated extension state (EPC)
AL LT LV MK RO SI
DOCDB simple family (publication)
WO 0070086 A1 20001123; WO 0070086 A9 20010419; AT E466101 T1 20100515; AT E541947 T1 20120215; AT E541948 T1 20120215; AU 4427100 A 20001205; CA 2373290 A1 20001123; CA 2373290 C 20120417; CN 103255206 A 20130821; CN 1350592 A 20020522; CN 1350592 B 20140219; DE 60044294 D1 20100610; EP 1179090 A1 20020213; EP 1179090 B1 20100428; EP 2230317 A1 20100922; EP 2230317 B1 20120118; EP 2236629 A1 20101006; EP 2236629 B1 20120118; EP 2360276 A1 20110824; EP 2365097 A1 20110914; ES 2345243 T3 20100920; ES 2380567 T3 20120516; ES 2380577 T3 20120516; JP 2003526334 A 20030909; JP 2011062202 A 20110331; JP 2014195456 A 20141016; JP 5902749 B2 20160413; MX PA01011635 A 20030910; US 2011111398 A1 20110512; US 2012258453 A1 20121011; US 2013296179 A1 20131107; US 7972777 B1 20110705; US 8946127 B2 20150203; ZA 200109333 B 20030129
DOCDB simple family (application)
IE 0000066 W 20000515; AT 00925552 T 20000515; AT 10160953 T 20000515; AT 10160954 T 20000515; AU 4427100 A 20000515; CA 2373290 A 20000515; CN 00807503 A 20000515; CN 201210580879 A 20000515; DE 60044294 T 20000515; EP 00925552 A 20000515; EP 10160953 A 20000515; EP 10160954 A 20000515; EP 10184640 A 20000515; EP 10184687 A 20000515; ES 00925552 T 20000515; ES 10160953 T 20000515; ES 10160954 T 20000515; JP 2000618491 A 20000515; JP 2010216091 A 20100927; JP 2014096195 A 20140507; MX PA01011635 A 20000515; US 201313784121 A 20130304; US 77248510 A 20100503; US 77274110 A 20100503; US 95996400 A 20000515; ZA 200109333 A 20011113