EP 2235157 A1 20101006 - METHOD AND DEVICE FOR THE DIGESTION OF BIOLOGICAL CELLS
Title (en)
METHOD AND DEVICE FOR THE DIGESTION OF BIOLOGICAL CELLS
Title (de)
VERFAHREN UND VORRICHTUNG FÜR DAS AUFSCHLIEßEN VON BIOLOGISCHEN ZELLEN
Title (fr)
PROCÉDÉ ET DISPOSITIF POUR LA DÉSAGRÉGATION DE CELLULES BIOLOGIQUES
Publication
Application
Priority
- EP 2009050440 W 20090115
- EP 08150595 A 20080124
- EP 09703275 A 20090115
Abstract (en)
[origin: EP2083069A1] The method comprises delivering cells together with pellet or particle (4) and a lyse buffer in a vessel (1) and then digesting through a magnetic stirrer with stirrer elements through bacteria or fungus. A stirrer rod (3) of the stirrer is arranged transversely relative to the base (5) of the vessel and is horizontally arranged. A mixing tool or stirrer element is rotated for stirring maximum 10 minutes with or without changing of the rotation direction. The buffer solution is sucked off with the cells present in the buffer solution in connection to the digestion over a line (7). The method comprises delivering cells together with pellet or particle (4) and a lyse buffer in a vessel (1) and then digesting through a magnetic stirrer with stirrer elements through bacteria or fungus. A stirrer rod (3) of the stirrer is arranged transversely relative to the base (5) of the vessel and is horizontally arranged. A mixing tool or stirrer element is rotated for stirring maximum 10 minutes with or without changing of the rotation direction. The buffer solution is sucked off with the cells present in the buffer solution during the digestion over a line (7) that ends at the base of the vessel. The stirrer element is driven related to the vessel in contact-free manner. The solution consists of additional enzymes for the cell. An independent claim is included for a device for the digestion of biological cells.
IPC 8 full level
C12N 1/06 (2006.01); C12M 1/33 (2006.01); C12M 3/08 (2006.01)
CPC (source: EP US)
C12N 1/066 (2013.01 - EP US)
Citation (search report)
See references of WO 2009092662A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA RS
DOCDB simple family (publication)
EP 2083069 A1 20090729; EP 2235157 A1 20101006; US 2011189759 A1 20110804; WO 2009092662 A1 20090730
DOCDB simple family (application)
EP 08150595 A 20080124; EP 09703275 A 20090115; EP 2009050440 W 20090115; US 86352309 A 20090115