Global Patent Index - EP 2236613 A1

EP 2236613 A1 20101006 - Biochemical synthesis of 1,4-butanediamine

Title (en)

Biochemical synthesis of 1,4-butanediamine

Title (de)

Biochemische Synthese von 1,4-Butandiamin

Title (fr)

Synthèse biochimique de 1,4-butanediamine

Publication

EP 2236613 A1 20101006 (EN)

Application

EP 10164437 A 20050711

Priority

  • EP 05774363 A 20050711
  • EP 04077047 A 20040715
  • EP 10164437 A 20050711

Abstract (en)

The invention relates to a process for biochemical synthesis of 1,4-butanediamine in a microorganism having an increased level of an ornithine decarboxylase activity (increased ODC activity) as compared to the native level of the ornithine decarboxylase activity, wherein the increased ODC activity is obtained by means of overexpression of an ornithine decarboxylase encoding gene with increased translational and/or transcriptional efficiency, and wherein 1 ,4-butanediamine produced in the microorganism is excreted into a fermentation broth, and is recovered from the fermentation broth. In preferred embodiments also increased enzyme activity is obtained by of overexpression of either (i) an arginine decarboxylase encoding gene spa4 and an agmatinase encoding gene speB ; or (ii) an arginine decarboxylase encoding gene speA and an agmatine iminohydrolase encoding gene aguA, and an N-carbamoylputrescine amidohydrolase encoding gene aguB, and optionally also an agmatinase encoding gene speB. The invention also relates to vectors, plasmids and hosts carrying, at an increased level of activity, one or more of the enzyme activities as mentioned.

IPC 8 full level

C12N 1/21 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12P 13/00 (2006.01)

CPC (source: EP US)

C12N 9/1029 (2013.01 - EP US); C12N 9/78 (2013.01 - EP US); C12N 9/80 (2013.01 - EP US); C12N 9/88 (2013.01 - EP US); C12P 13/001 (2013.01 - EP US); C12Y 305/01053 (2013.01 - EP US); C12Y 305/03011 (2013.01 - EP US); C12Y 305/03012 (2013.01 - EP US); C12Y 401/01017 (2013.01 - EP US); Y02P 20/52 (2015.11 - US)

Citation (applicant)

  • US 2130948 A 19380920 - HUME CAROTHERS WALLACE
  • EP 0726240 A1 19960814 - TAGUIDELL S L [ES]
  • FUKUCHI ET AL., J. BIOL. CHEM., vol. 270, 1995, pages 18831 - 18835
  • SUZUKI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 91, 1994, pages 8930 - 8934
  • LIMSUWUM ET AL., J. BACTERIOL. VOI., vol. 182, 2000, pages 5373 - 5380
  • KASHIWAGI ET AL., J. BACTERIOL. VOI., vol. 170, 1988, pages 3131 - 3135
  • BOYLE ET AL., METHODS IN ENZYMOLOGY, vol. 94, 1983, pages 117 - 121
  • OSTERMAN, A. L. ET AL., BIOCHEMISTRY, vol. 33, 1994, pages 13662 - 13667
  • JANOWITZ ET AL., FEBS LETTERS, vol. 544, 2003, pages 258 - 261
  • NAKADA ET AL., MICROBIOLOGY, vol. 149, 2003, pages 707 - 714
  • ZEPPENFELD ET AL., J BACTERIOL., vol. 182, 2000, pages 4443 - 4452
  • FÜRSTE, J. P. ET AL., GENE, vol. 48, 1986, pages 119 - 131
  • FÜRSTE ET AL., GENE, vol. 48, 1986, pages 119 - 131

Citation (search report)

  • [X] WO 03050296 A2 20030619 - AVENTIS PHARMA GMBH [DE]
  • [AD] EP 0726240 A1 19960814 - TAGUIDELL S L [ES]
  • [A] WO 0111062 A2 20010215 - JOHN INNES CT [GB]
  • [XY] MACRAE M ET AL: "Complementation of a polyamine-deficient Escherichia coli mutant by expression of mouse ornithine decarboxylase.", MOLECULAR AND CELLULAR BIOLOGY, vol. 7, no. 1, January 1987 (1987-01-01), pages 564 - 567, XP002300275, ISSN: 0270-7306
  • [X] GUPTA R ET AL: "Effect of spermidine on the in vivo degradation of ornithine decarboxylase in Saccharomyces cerevisiae", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 98, no. 19, 11 September 2001 (2001-09-11), pages 10620 - 10623, XP002313717, ISSN: 0027-8424
  • [X] PANAGIOTIDIS C A ET AL: "Relationship of the expression of the S20 and L34 ribosomal proteins to polyamine biosynthesis in Escherichia coli.", THE INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, vol. 27, no. 2, February 1995 (1995-02-01), pages 157 - 168, XP002313718, ISSN: 1357-2725
  • [X] KLEIN R D ET AL: "Haemonchus contortus: Cloning and functional expression of a cDNA encoding ornithine decarboxylase and development of a screen for inhibitors", EXPERIMENTAL PARASITOLOGY, vol. 87, no. 3, November 1997 (1997-11-01), pages 171 - 184, XP002313719, ISSN: 0014-4894
  • [X] FONZI W A ET AL: "Expression of the gene for ornithine decarboxylase of Saccharomyces cerevisiae in Escherichia coli.", MOLECULAR AND CELLULAR BIOLOGY, vol. 5, no. 1, January 1985 (1985-01-01), pages 161 - 166, XP008040911, ISSN: 0270-7306
  • [A] PANTOJA-HERNANDEZ M A ET AL: "Expression of ornithine decarboxylase of Coccidioides immitis in three Escherichia coli strains carrying the lambda DE3 lysogen and an E. coli EWH319 strain odc- null mutant.", BIOTECHNOLOGY LETTERS, vol. 26, no. 1, January 2004 (2004-01-01), pages 75 - 78, XP002313720, ISSN: 0141-5492
  • [A] NIEMANN G ET AL: "Panagrellus redivivus ornithine decarboxylase: Structure of the gene, expression in Escherichia coli and characterization of the recombinant protein", BIOCHEMICAL JOURNAL, vol. 317, no. 1, 1996, pages 135 - 140, XP002313721, ISSN: 0264-6021
  • [A] MICHAEL A J ET AL: "Molecular cloning and functional identification of a plant ornithine decarboxylase cDNA", BIOCHEMICAL JOURNAL, vol. 314, no. 1, 1996, pages 241 - 248, XP002313722, ISSN: 0264-6021
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  • [A] PHILLIPS M A ET AL: "Trypanosoma brucei ornithine decarboxylase: enzyme purification, characterization, and expression in Escherichia coli", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 263, no. 34, 1988, pages 17933 - 17941, XP002313724, ISSN: 0021-9258
  • [A] KLEIN R D ET AL: "Reconstitution of a bacterial/plant polyamine biosynthesis pathway in Saccharomyces cerevisiae", MICROBIOLOGY, vol. 145, no. 2, February 1999 (1999-02-01), pages 301 - 307, XP002232122, ISSN: 1350-0872
  • [YD] KASHIWAGI K ET AL: "Adjustment of polyamine contents in Escherichia coli.", JOURNAL OF BACTERIOLOGY. JUL 1988, vol. 170, no. 7, July 1988 (1988-07-01), pages 3131 - 3135, XP008036641, ISSN: 0021-9193
  • [Y] BOYLE S M ET AL: "Expression of the cloned gene encoding the putrescine biosynthetic enzymes and methionine adenosyltransferase of Escherichia coli (speA, speB, speC and metK)", GENE, vol. 30, no. 1-3, 1984, pages 129 - 136, XP002232121, ISSN: 0378-1119
  • [Y] KASHIWAGI K ET AL: "Coexistence of the genes for putrescine transport protein and ornithine decarboxylase at 16 min on Escherichia coli chromosome", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 266, no. 31, 1991, pages 20922 - 20927, XP002318344, ISSN: 0021-9258
  • [Y] NAKADA Y ET AL: "Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway.", MICROBIOLOGY, vol. 149, no. 3, March 2003 (2003-03-01), pages 707 - 714, XP002300276, ISSN: 1350-0872
  • [A] CUNIN R ET AL: "Biosynthesis and metabolism of arginine in bacteria", MICROBIOLOGICAL REVIEWS, vol. 3, no. 50, September 1986 (1986-09-01), pages 314 - 352, XP001068304, ISSN: 0146-0749
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  • [A] COHEN S S: "A guide to the polyamines - Biosynthesis and metabolism of diamines in bacteria", 1998, OXFORD UNIVERSITY PRESS, OXFORD, ISBN: 0195110641, XP002300280
  • [AD] FUKUCHI J-I ET AL: "Decrease in cell viability due to the accumulation of spermidine in spermidine acetyltransferase-deficient mutant of Escherichia coli", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 270, no. 32, 1995, pages 18831 - 18835, XP002300277, ISSN: 0021-9258
  • [AD] SUZUKI T ET AL: "Antizyme protects against abnormal accumulation and toxicity of polyamines in ornithine decarboxylase-overproducing cells", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 91, no. 19, 1994, pages 8930 - 8934, XP002300278, ISSN: 0027-8424

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

DOCDB simple family (publication)

WO 2006005604 A1 20060119; AT E504658 T1 20110415; AU 2005261862 A1 20060119; AU 2005261862 B2 20100826; BR PI0513362 A 20080506; CA 2571531 A1 20060119; CA 2571531 C 20160216; CN 101006183 A 20070725; CN 101006183 B 20110727; DE 602005027356 D1 20110519; EA 011232 B1 20090227; EA 200700330 A1 20070629; EP 1781799 A1 20070509; EP 1781799 B1 20110406; EP 2236613 A1 20101006; EP 2236613 B1 20150527; JP 2008505652 A 20080228; JP 2012130343 A 20120712; JP 5010470 B2 20120829; MX 2007000565 A 20070402; MY 148119 A 20130228; NZ 552628 A 20090925; PL 1781799 T3 20110729; PL 2236613 T3 20151130; TW 200613558 A 20060501; TW I354026 B 20111211; UA 91686 C2 20100825; US 2009275093 A1 20091105; US 2016319311 A1 20161103; US 9523083 B2 20161220; ZA 200700072 B 20080528

DOCDB simple family (application)

EP 2005007608 W 20050711; AT 05774363 T 20050711; AU 2005261862 A 20050711; BR PI0513362 A 20050711; CA 2571531 A 20050711; CN 200580023807 A 20050711; DE 602005027356 T 20050711; EA 200700330 A 20050711; EP 05774363 A 20050711; EP 10164437 A 20050711; JP 2007520745 A 20050711; JP 2012010241 A 20120120; MX 2007000565 A 20050711; MY PI20053245 A 20050715; NZ 55262805 A 20050711; PL 05774363 T 20050711; PL 10164437 T 20050711; TW 94124158 A 20050715; UA A200701586 A 20050711; US 201615212385 A 20160718; US 63262505 A 20050711; ZA 200700072 A 20070102