EP 2245160 A1 20101103 - CELL SURFACE DISPLAY OF PROTEINS
Title (en)
CELL SURFACE DISPLAY OF PROTEINS
Title (de)
ZELLOBERFLÄCHENPRÄSENTATION VON PROTEINEN
Title (fr)
PRÉSENTATION DE PROTÉINES SUR LA SURFACE CELLULAIRE
Publication
Application
Priority
- IB 2009000086 W 20090121
- ZA 200800623 A 20080122
Abstract (en)
[origin: WO2009093118A1] A yeast cell surface display system is described comprising a scaffoldin protein from Clostridium which includes at least one cohesin domain capable of binding to a dockerin domain, the scaffoldin protein displayable on the surface of the yeast cell, such as S. cerevisiae, and an anchoring protein capable of tethering the scaffoldin protein the yeast cell surface, such as cell wall protein 1 (Cwp 1 ). Cwp 1 is fused to the C-terminus of the scaffoldin protein and a secretion signal, such as Trichoderma reesei XYN2 is fused to the N-terminus of the chimeric scaffoldin protein. The scaffoldin protein is chimeric and comprises at least one C. cellulolyticum cohesin domain and at least one C. Thermocellum cohesin domain. The invention also includes a vector for expression of such a fusion protein.
IPC 8 full level
C12N 15/31 (2006.01); C07K 19/00 (2006.01); C12N 1/18 (2006.01); C12N 1/19 (2006.01); C12N 15/09 (2006.01); C12N 15/81 (2006.01); C12P 21/00 (2006.01)
CPC (source: EP)
C07K 14/33 (2013.01); C12N 15/62 (2013.01); C12N 15/625 (2013.01); C12N 15/81 (2013.01); C07K 2319/02 (2013.01); C07K 2319/74 (2013.01)
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR
Designated extension state (EPC)
AL BA RS
DOCDB simple family (publication)
WO 2009093118 A1 20090730; WO 2009093118 A9 20091105; EP 2245160 A1 20101103; EP 2245160 A4 20110330
DOCDB simple family (application)
IB 2009000086 W 20090121; EP 09703905 A 20090121