Global Patent Index - EP 2247727 A2

EP 2247727 A2 20101110 - METHOD FOR ARCHIVING AND CLONAL EXPANSION

Title (en)

METHOD FOR ARCHIVING AND CLONAL EXPANSION

Title (de)

VERFAHREN ZUR ARCHIVIERUNG UND KLONALEN EXPANSION

Title (fr)

PROCÉDÉ POUR ARCHIVAGE ET EXPANSION CLONALE

Publication

EP 2247727 A2 20101110 (EN)

Application

EP 09711405 A 20090212

Priority

  • US 2009033936 W 20090212
  • US 2814608 P 20080212
  • US 3869608 P 20080321
  • US 7499108 P 20080623
  • US 8581108 P 20080801

Abstract (en)

[origin: WO2009102878A2] The present method provides methods, libraries, and kits related to the archiving and clonal expansion of sequences related to target polynucleotide sequences. The method allow for the attachment of polynucleotides with defined 3' and or 5' sequences to solid surfaces. The polynucleotides attached to the solid substrates can be stored or archived as libraries and can subsequently be retrieved for analysis, for example by clonal expansion. In some embodiments, nucleotides attached to solid surfaces can be used for sequencing of nucleotide sequences related to target RNA or target RNA. The methods are applicable to total RNA and/or total DNA analysis.

IPC 8 full level

C12Q 1/68 (2006.01)

CPC (source: EP)

C12Q 1/6844 (2013.01)

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR

Designated extension state (EPC)

AL BA RS

DOCDB simple family (publication)

WO 2009102878 A2 20090820; WO 2009102878 A3 20100121; EP 2247727 A2 20101110; EP 2247727 A4 20110803; EP 2250283 A2 20101117; WO 2009102896 A2 20090820; WO 2009102896 A3 20091119

DOCDB simple family (application)

US 2009033936 W 20090212; EP 09709858 A 20090212; EP 09711405 A 20090212; US 2009033964 W 20090212