Global Patent Index - EP 2250253 A1

EP 2250253 A1 20101117 - SYSTEM AND METHOD FOR THE CLONAL CULTURE OF EPITHELIAL CELLS AND APPLICATIONS THEREOF

Title (en)

SYSTEM AND METHOD FOR THE CLONAL CULTURE OF EPITHELIAL CELLS AND APPLICATIONS THEREOF

Title (de)

SYSTEM UND VERFAHREN ZUR KLONALEN KULTUR VON EPITHELZELLEN UND ANWENDUNGEN DAVON

Title (fr)

SYSTEME ET PROCEDE DE CULTURE CLONALE DE CELLULES EPITHELIALES ET LEURS APPLICATIONS

Publication

EP 2250253 A1 20101117 (FR)

Application

EP 09711589 A 20090218

Priority

  • EP 2009051912 W 20090218
  • FR 0851054 A 20080219

Abstract (en)

[origin: WO2009103731A1] The invention relates to means and methods for evaluating and using the specific properties of a particular epithelial cell present in a biological sample. Accordingly, the invention relates to a system for the culture of epithelial cells, in which at least one clonal culture is sown with a single epithelial cell directly extracted from a biological sample of epithelial tissue. The invention also relates to a method for the culture of epithelial cells, that particularly comprises the production of clonal cultures, each being sown with a distinct and unique epithelial cell directly extracted from a biological sample of epithelial tissue, the evaluation of the cellular growth in the clonal cultures, and advantageously the analysis of the capacity of the cellular material from the clonal cultures to reconstruct a three-dimensional epithelium representative of native tissue. The invention is adapted for the parallel implementation of a very large number of clonal cultures, in particular for making large-scale tests.

IPC 1-7

C12N 5/06

IPC 8 full level

C12N 5/07 (2010.01); C12N 5/071 (2010.01); C12N 5/074 (2010.01)

CPC (source: EP US)

C12N 5/0629 (2013.01 - EP US); C12N 2503/00 (2013.01 - EP US); C12N 2503/04 (2013.01 - EP US); C12N 2503/06 (2013.01 - EP US)

Citation (search report)

See references of WO 2009103731A1

Citation (examination)

  • A. SUZUKI ET AL: "Prospective Isolation of Multipotent Pancreatic Progenitors Using Flow-Cytometric Cell Sorting", DIABETES, vol. 53, no. 8, 1 January 2004 (2004-01-01), pages 2143 - 2152, XP055016787, ISSN: 0012-1797, DOI: 10.2337/diabetes.53.8.2143
  • MANON E J FRANSSEN ET AL: "Phenotypical and Functional Differences in Germinative Subpopulations Derived from Normal and Psoriatic Epidermis", JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 124, no. 2, 1 February 2005 (2005-02-01), pages 373 - 383, XP055129563, ISSN: 0022-202X, DOI: 10.1111/j.0022-202X.2004.23612.x
  • OSHIMA ET AL: "Isolation of Mouse Pancreatic Ductal Progenitor Cells Expressing CD133 and c-Met by Flow Cytometric Cell Sorting", GASTROENTEROLOGY, ELSEVIER, PHILADELPHIA, PA, vol. 132, no. 2, 19 February 2007 (2007-02-19), pages 720 - 732, XP005920939, ISSN: 0016-5085, DOI: 10.1053/J.GASTRO.2006.11.027
  • DEREK DAVIES ED - MACEY MARION G: "Chapter 11: Cell sorting by flow cytometry", 3 July 2007, FLOW CYTOMETRY: PRINCIPLES AND APPLICATION, HUMANA PRESS, TOTOWA, NJ, PAGE(S) 257 - 276, ISBN: 978-1-58829-691-7, XP002698388
  • KAREN A. HOLBROOK ET AL: "Phenotypic Expression of Epidermal Cells in Vitro: A Review.", JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 81, no. s1, 1 July 1983 (1983-07-01), pages 11s - 24s, XP055173246, ISSN: 0022-202X, DOI: 10.1111/1523-1747.ep12540003
  • TREMPUS C S ET AL: "Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34", THE JOURNAL OF INVESTIGATIVE DERMATOLOGY, NATURE PUBLISHING GROUP, GB, vol. 120, no. 4, 1 April 2003 (2003-04-01), pages 501 - 511, XP003009211, ISSN: 0022-202X, DOI: 10.1046/J.1523-1747.2003.12088.X
  • RACHIDI ET AL: "Sensing radiosensitivity of human epidermal stem cells", RADIOTHERAPY AND ONCOLOGY, ELSEVIER, IRELAND, vol. 83, no. 3, 1 June 2007 (2007-06-01), pages 267 - 276, XP022134068, ISSN: 0167-8140, DOI: 10.1016/J.RADONC.2007.05.007

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR

Designated extension state (EPC)

AL BA RS

DOCDB simple family (publication)

FR 2927633 A1 20090821; FR 2927633 B1 20120713; EP 2250253 A1 20101117; JP 2011512146 A 20110421; US 2010331197 A1 20101230; US 2012264623 A2 20121018; WO 2009103731 A1 20090827

DOCDB simple family (application)

FR 0851054 A 20080219; EP 09711589 A 20090218; EP 2009051912 W 20090218; JP 2010547167 A 20090218; US 91802809 A 20090218