Global Patent Index - EP 2274973 A4

EP 2274973 A4 20110622 - AGROBACTERIUM-MEDIATED METHOD FOR PRODUCING TRANSFORMED PLANT

Title (en)

AGROBACTERIUM-MEDIATED METHOD FOR PRODUCING TRANSFORMED PLANT

Title (de)

AGROBAKTERIUM-VERMITTELTES VERFAHREN ZUR HERSTELLUNG TRANSFORMIERTER PFLANZEN

Title (fr)

PROCEDE AU MOYEN D'AGROBACTERIUM DE PRODUCTION D'UNE PLANTE TRANSFORMEE

Publication

EP 2274973 A4 20110622 (EN)

Application

EP 09727983 A 20090324

Priority

  • JP 2009055791 W 20090324
  • JP 2008094049 A 20080331

Abstract (en)

[origin: EP2274973A1] The present invention aims to provide a novel Agrobacterium-mediated method for producing a transformed plant. The method of the present invention is an Agrobacterium-mediated method for producing a transformed plant, which comprises: (i) a coculture step for culturing an Agrobacterium-inoculated plant material with a coculture medium; and (ii) a regeneration step for culturing the tissue obtained in (i) with a regeneration medium, either without callus growth culture or after callus growth culture, to thereby regenerate a whole plant, wherein 1) said method comprises transformation enhancement, and wherein 2) said method does not comprise selection of transformed cells based on the properties of a nucleic acid to be introduced by Agrobacterium in any step from coculture to regeneration.

IPC 8 full level

A01H 1/00 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01)

CPC (source: EP US)

C12N 15/8205 (2013.01 - EP US); C12N 15/821 (2013.01 - EP US); C12N 15/8212 (2013.01 - EP US)

Citation (search report)

  • [YD] WO 9854961 A2 19981210 - NOVARTIS AG [CH], et al
  • [YD] WO 0212521 A1 20020214 - JAPAN TOBACCO INC [JP], et al
  • [XY] WO 03010319 A2 20030206 - AVEBE COOP VERKOOP PROD [NL], et al
  • [Y] KR 20050028255 A 20050322 - NEXGEN BIOTECHNOLOGIES INC [KR]
  • [YP] WO 2008119136 A1 20081009 - UNIV QUEENSLAND [AU], et al
  • [XY] VETTEN DE N ET AL: "A transformation method for obtaining marker - free plants of a cross-pollinating and vegetatively propagated crop", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 21, 1 April 2003 (2003-04-01), pages 439 - 442, XP002236876, ISSN: 1087-0156, DOI: 10.1038/NBT801
  • [Y] ROMMENS C M: "All-native DNA transformation: a new approach to plant genetic engineering", TRENDS IN PLANT SCIENCE, ELSEVIER SCIENCE, OXFORD, GB, vol. 9, no. 9, 1 September 2004 (2004-09-01), pages 457 - 464, XP004602703, ISSN: 1360-1385, DOI: 10.1016/J.TPLANTS.2004.07.001
  • [XY] TROY WEEKS J ET AL: "Development of an in planta method for transformation of alfalfa (Medicago sativa)", TRANSGENIC RESEARCH, KLUWER ACADEMIC PUBLISHERS-PLENUM PUBLISHERS, NE, vol. 17, no. 4, 13 September 2007 (2007-09-13), pages 587 - 597, XP019616123, ISSN: 1573-9368
  • [XY] HONGGE JIA ET AL: "Direct creation of marker-free tobacco plants from agroinfiltrated leaf discs", PLANT CELL REPORTS, SPRINGER, BERLIN, DE, vol. 26, no. 11, 19 July 2007 (2007-07-19), pages 1961 - 1965, XP019561671, ISSN: 1432-203X, DOI: 10.1007/S00299-007-0403-Y
  • [Y] FANRONG HU ET AL: "Agrobacterium-mediated transformed transgenic triploid bermudagrass (Cynodon dactylon C. transvaalensis) plants are highly resistant to the glufosinate herbicide Liberty", PLANT CELL, TISSUE AND ORGAN CULTURE, KLUWER ACADEMIC PUBLISHERS, DO, vol. 83, no. 1, 1 October 2005 (2005-10-01), pages 13 - 19, XP019268636, ISSN: 1573-5044
  • [Y] KUMAR JITENDRA ET AL: "In vitro plant regeneration and genetic transformation of Dichanthium annulatum", DNA AND CELL BIOLOGY, vol. 24, no. 11, November 2005 (2005-11-01), pages 670 - 679, XP009148123, ISSN: 1044-5498
  • [YP] BAOCHUN LI ET AL: "Production of selectable marker-free transgenic tobacco plants using a non-selection approach: chimerism or escape, transgene inheritance, and efficiency", PLANT CELL REPORTS, SPRINGER, BERLIN, DE, vol. 28, no. 3, 19 November 2008 (2008-11-19), pages 373 - 386, XP019709111, ISSN: 1432-203X
  • See references of WO 2009122962A1

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR

DOCDB simple family (publication)

EP 2274973 A1 20110119; EP 2274973 A4 20110622; AU 2009232967 A1 20091008; AU 2009232967 A8 20101125; AU 2009232967 B2 20150402; CN 101983007 A 20110302; CN 101983007 B 20131106; HK 1154333 A1 20120420; JP 2011120478 A 20110623; US 2011030101 A1 20110203; US 2013125265 A1 20130516; US 8357836 B2 20130122; WO 2009122962 A1 20091008

DOCDB simple family (application)

EP 09727983 A 20090324; AU 2009232967 A 20090324; CN 200980112149 A 20090324; HK 11108593 A 20110816; JP 2008094049 A 20080331; JP 2009055791 W 20090324; US 201213717663 A 20121217; US 93552509 A 20090324