Title (en)

Time-resolved fluorescence energy transfer (TR-FRET) assay for detecting exposure to an infectious organism

Title (de)

Zeitaufgelöster Fluoreszenzenergietransfer-(TR-FRET-)test zum Nachweis eines Inkontaktkommens mit einem infektiösen Organismus

Title (fr)

Procédé de détection de l'exposition à un organisme infectieux par transfert d'énergie par résonance de type Förster en temps résolu (TR-FRET)

Publication

EP 2277047 B1 20141105 (EN)

Application

EP 09725807 A 20090327

Priority

• GB 2009050303 W 20090327
• GB 0805608 A 20080328

Abstract (en)

[origin: WO2009118570A2] A method for detecting the presence of a diagnostic moiety indicative of exposure to an infectious organism in a biological sample taken from a human or animal, said method comprising; (a) adding to said sample a first fluorescently labelled reagent which binds said diagnostic moiety, and a second fluorescently labelled reagent which either binds said diagnostic moiety in addition to said first fluorescently labelled reagent, or which binds the first fluorescently labelled reagent or a complex comprising the first fluorescently labelled reagent in competition to the said diagnostic moiety, wherein a label on one of the first or second fluorescently labelled reagent acts as a fluorescent energy donor compound and wherein the other of the first or second fluorescentiy labelled reagent acts as a fluorescent energy acceptor compound which is able to accept fluorescent energy from said donor compound; (b) exciting the fluorescent energy donor compound by illuminating with light of a wavelength which is absorbed by said fluorescent energy donor compound; (c) measuring fluorescent signal emitted by said fluorescent energy acceptor compound as a result of its absorption of the fluorescent energy from the donor compound after a time delay; and (d) relating the results to the presence or absence of diagnostic moiety in said sample.

IPC 8 full level

G01N 33/542 (2006.01); G01N 33/569 (2006.01); G01N 33/58 (2006.01)

CPC (source: EP US)

G01N 33/542 (2013.01 - EP US); G01N 2333/005 (2013.01 - EP US); G01N 2800/26 (2013.01 - EP US)

Citation (examination)

• WO 2005000883 A1 20050106 - AFFIBODY AB [SE], et al
• WO 2004041865 A2 20040521 - ABLYNX NV [BE], et al
• WO 2008048970 A2 20080424 - UNIV ARIZONA [US], et al
• NEWBY D T ET AL: "REAL-TIME PCR DETECTION OF BRUCELLA ABORTUS: A COMPARATIVE STUDY OFSYBR GREEN I, 5'-EXONUCLEASE, AND HYBRIDIZATION PROBE ASSAYS", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 69, no. 8, 1 August 2003 (2003-08-01), pages 4753 - 4759, XP008070970, ISSN: 0099-2240, DOI: 10.1128/AEM.69.8.4753-4759.2003
• BINZ H K ET AL: "Engineered proteins as specific binding reagents", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 16, no. 4, 1 August 2005 (2005-08-01), pages 459 - 469, XP027676733, ISSN: 0958-1669, [retrieved on 20050801]
• NORD K ET AL: "Binding proteins selected from combinatorial libraries af an alpha-helical bacterial receptor domain", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 15, no. 8, 1 August 1997 (1997-08-01), pages 772 - 777, XP002149252, ISSN: 1087-0156
• SILVERMAN JOSHUA ET AL: "Multivalent avimer proteins evolved by exon shuffling of a family of human receptor domains", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 23, no. 12, 20 November 2005 (2005-11-20), pages 1556 - 1561, XP009088629, ISSN: 1087-0156, DOI: 10.1038/NBT1166

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR

DOCDB simple family (publication)

WO 2009118570 A2 20091001; WO 2009118570 A3 20091223; EP 2277047 A2 20110126; EP 2277047 B1 20141105; EP 2277047 B8 20141217; EP 2618148 A1 20130724; EP 2618148 B1 20141105; EP 2618148 B8 20141217; GB 0805608 D0 20080430; US 2011039256 A1 20110217

DOCDB simple family (application)

GB 2009050303 W 20090327; EP 09725807 A 20090327; EP 13161174 A 20090327; GB 0805608 A 20080328; US 92157809 A 20090327