EP 2284592 A1 20110216 - Fluorescence imaging
Title (en)
Fluorescence imaging
Title (de)
Fluoreszenzbildgebung
Title (fr)
Imagerie par fluorescence
Publication
Application
Priority
US 53438709 A 20090803
Abstract (en)
A method for generating a fluorescence microscopy image of a sample. The method comprises a step of obtaining a series of fluorescence microscopy images of the sample for a plurality of different focal plane depths in the sample and different exposure times. The images may be obtained directly from a microscope, or from a library of images. Images comprising the series of fluorescence microscopy images are combined to form a single resultant fluorescence microscopy image of the sample. The single resultant image may then be analysed as if it were a conventional fluorescence microscopy image, e.g. by visual inspection or numerical processing. However, because the resultant image is based on images from a range of focal plane depths and exposure times, different structures that appear under only certain conditions (i.e. in only certain ones of the original series of fluorescence microscopy images) can appear together in a single image, thus simplifying visualisation, interpretation and analysis of the sample.
IPC 8 full level
G01N 21/64 (2006.01); G02B 21/36 (2006.01); G06T 5/50 (2006.01)
CPC (source: EP US)
G01N 21/6458 (2013.01 - EP US); G02B 21/16 (2013.01 - EP US); G06T 5/50 (2013.01 - EP US); G06T 2207/10121 (2013.01 - EP US); G06T 2207/30101 (2013.01 - EP US)
Citation (search report)
- [XI] US 2008212866 A1 20080904 - LETT G SCOTT [US], et al
- [XI] WO 2008019299 A2 20080214 - IKONISYS INC [US], et al
- [XA] US 2006257013 A1 20061116 - RAMM PETER [CA], et al
- [XA] US 2007243545 A1 20071018 - KILPATRICK MICHAEL [US], et al
- [A] GRIGORYAN A M ET AL: "Morphological spot counting from stacked images for automated analysis of gene copy numbers by fluorescence in situ hybridization", JOURNAL OF BIOMEDICAL OPTICS, SPIE - INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING, US LNKD- DOI:10.1117/1.1428292, vol. 7, no. 1, 1 January 2002 (2002-01-01), pages 109 - 122, XP008102322, ISSN: 1083-3668
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
Designated extension state (EPC)
BA ME RS
DOCDB simple family (publication)
EP 2284592 A1 20110216; EP 2284592 B1 20210721; EP 3029507 A1 20160608; EP 3029507 B1 20171018; US 2011025880 A1 20110203; US 8310531 B2 20121113
DOCDB simple family (application)
EP 10250910 A 20100512; EP 15193495 A 20100512; US 53438709 A 20090803