Global Patent Index - EP 2326431 A1

EP 2326431 A1 20110601 - METHOD FOR NORMALIZING THE CONTENTS OF BIOMOLECULES IN A SAMPLE

Title (en)

METHOD FOR NORMALIZING THE CONTENTS OF BIOMOLECULES IN A SAMPLE

Title (de)

VERFAHREN ZUR NORMIERUNG DES GEHALTS VON BIOMOLEKÜLEN IN EINER PROBE

Title (fr)

PROCÉDÉ DE NORMALISATION DU CONTENU DE BIOMOLÉCULES D'UN ÉCHANTILLON

Publication

EP 2326431 A1 20110601 (DE)

Application

EP 09783068 A 20090916

Priority

  • EP 2009061990 W 20090916
  • DE 102008047790 A 20080917

Abstract (en)

[origin: WO2010031784A1] The present invention relates to a method for normalizing the contents of biomolecules in a sample, comprising the following steps: a) preparing a reaction vessel with a vessel surface that is functionalized at least in sections - preferably on the inside of the vessel - in such a way that the surface can reversibly bind biomolecules under high salt conditions, b) executing at least one sample preparation step, c) binding biomolecules from the prepared sample to the vessel surface ("binding and normalizing step") under high salt conditions, d) optionally washing ("washing step"), and e) executing at least one subsequent reaction. The application also relates to a reaction vessel as is used in the above method.

IPC 8 full level

B05D 7/24 (2006.01); C12Q 1/68 (2006.01)

CPC (source: EP US)

C12Q 1/6806 (2013.01 - EP US)

Citation (search report)

See references of WO 2010031784A1

Citation (examination)

  • WO 0114590 A2 20010301 - PROMEGA CORP [US]
  • WO 2006081324 A2 20060803 - REED MICHAEL W [US], et al
  • BOOM R ET AL: "RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC ACIDS", JOURNAL OF CLINICAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC, US, vol. 28, no. 3, 1 March 1990 (1990-03-01), pages 495 - 503, XP000607196, ISSN: 0095-1137
  • MELZAK K A ET AL: "Driving Forces for DNA Adsorption to Silica in Perchlorate Solutions", JOURNAL OF COLLOID AND INTERFACE SCIENCE, ACADEMIC PRESS, NEW YORK, NY, US, vol. 181, no. 2, 10 August 1996 (1996-08-10), pages 635 - 644, XP027490744, ISSN: 0021-9797, [retrieved on 19960810], DOI: 10.1006/JCIS.1996.0421

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

Designated extension state (EPC)

AL BA RS

DOCDB simple family (publication)

WO 2010031784 A1 20100325; AU 2009294655 A1 20100325; AU 2009294655 B2 20140417; CN 102159333 A 20110817; DE 102008047790 A1 20100415; EP 2326431 A1 20110601; JP 2012502642 A 20120202; US 2011171656 A1 20110714

DOCDB simple family (application)

EP 2009061990 W 20090916; AU 2009294655 A 20090916; CN 200980137018 A 20090916; DE 102008047790 A 20080917; EP 09783068 A 20090916; JP 2011527310 A 20090916; US 200913063598 A 20090916