EP 2326431 A1 20110601 - METHOD FOR NORMALIZING THE CONTENTS OF BIOMOLECULES IN A SAMPLE
Title (en)
METHOD FOR NORMALIZING THE CONTENTS OF BIOMOLECULES IN A SAMPLE
Title (de)
VERFAHREN ZUR NORMIERUNG DES GEHALTS VON BIOMOLEKÜLEN IN EINER PROBE
Title (fr)
PROCÉDÉ DE NORMALISATION DU CONTENU DE BIOMOLÉCULES D'UN ÉCHANTILLON
Publication
Application
Priority
- EP 2009061990 W 20090916
- DE 102008047790 A 20080917
Abstract (en)
[origin: WO2010031784A1] The present invention relates to a method for normalizing the contents of biomolecules in a sample, comprising the following steps: a) preparing a reaction vessel with a vessel surface that is functionalized at least in sections - preferably on the inside of the vessel - in such a way that the surface can reversibly bind biomolecules under high salt conditions, b) executing at least one sample preparation step, c) binding biomolecules from the prepared sample to the vessel surface ("binding and normalizing step") under high salt conditions, d) optionally washing ("washing step"), and e) executing at least one subsequent reaction. The application also relates to a reaction vessel as is used in the above method.
IPC 8 full level
CPC (source: EP US)
C12Q 1/6806 (2013.01 - EP US)
Citation (search report)
See references of WO 2010031784A1
Citation (examination)
- WO 0114590 A2 20010301 - PROMEGA CORP [US]
- WO 2006081324 A2 20060803 - REED MICHAEL W [US], et al
- BOOM R ET AL: "RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC ACIDS", JOURNAL OF CLINICAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, DC, US, vol. 28, no. 3, 1 March 1990 (1990-03-01), pages 495 - 503, XP000607196, ISSN: 0095-1137
- MELZAK K A ET AL: "Driving Forces for DNA Adsorption to Silica in Perchlorate Solutions", JOURNAL OF COLLOID AND INTERFACE SCIENCE, ACADEMIC PRESS, NEW YORK, NY, US, vol. 181, no. 2, 10 August 1996 (1996-08-10), pages 635 - 644, XP027490744, ISSN: 0021-9797, [retrieved on 19960810], DOI: 10.1006/JCIS.1996.0421
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
Designated extension state (EPC)
AL BA RS
DOCDB simple family (publication)
WO 2010031784 A1 20100325; AU 2009294655 A1 20100325; AU 2009294655 B2 20140417; CN 102159333 A 20110817; DE 102008047790 A1 20100415; EP 2326431 A1 20110601; JP 2012502642 A 20120202; US 2011171656 A1 20110714
DOCDB simple family (application)
EP 2009061990 W 20090916; AU 2009294655 A 20090916; CN 200980137018 A 20090916; DE 102008047790 A 20080917; EP 09783068 A 20090916; JP 2011527310 A 20090916; US 200913063598 A 20090916