EP 2354222 A1 20110810 - Cellulase fusion protein and heterologous cellulase fusion construct encoding the same

Title (en)

Cellulase fusion protein and heterologous cellulase fusion construct encoding the same

Title (de)

Cellulase-Fusionsprotein und heterologes Cellulase-Fusionskonstrukt, das dieses Fusionsprotein kodiert

Title (fr)

Protéine de fusion de cellulase et construction de fusion de cellulose hétérologue la codant

Publication

EP 2354222 A1 20110810 (EN)

Application

EP 10010133 A 20050325

Priority

EP 05744703 A 20050325
US 55671104 P 20040325

Abstract (en)

The present invention relates to a heterologous cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a first catalytic domain derived from a fungal exo-cellobiohydrolase and a second catalytic domain derived from a cellulase enzyme. The invention also relate to vectors and fungal host cells comprising the heterologous cellulase fusion construct as well as methods for producing said cellulase fusion protein and enzymatic cellulase compositions.

IPC 8 full level

C12N 9/42 (2006.01); C12N 15/62 (2006.01); C12N 15/80 (2006.01); C12P 21/02 (2006.01); C12P 21/06 (2006.01)

CPC (source: EP)

C12N 9/2437 (2013.01); C12N 15/80 (2013.01); C12Y 302/01004 (2013.01); C12Y 302/01091 (2013.01); C07K 2319/02 (2013.01)

Citation (applicant)

US 4435307 A 19840306 - BARBESGAARD PEDER O [DK], et al
GB 2095275 A 19820929 - KAO CORP
GB 2094826 A 19820922 - KAO CORP
US 5648263 A 19970715 - SCHUELEIN MARTIN [DK], et al
US 5691178 A 19971125 - SCHUELEIN MARTIN [DK], et al
US 5776757 A 19980707 - SCHUELEIN MARTIN [DK], et al
GB 1358599 A 19740703 - MONACELLI UMBERTO
WO 9105039 A1 19910418 - MIDWEST RESEARCH INST [US]
WO 9315186 A1 19930805 - MIDWEST RESEARCH INST [US]
US 5275944 A 19940104 - HIMMEL MICHAEL E [US], et al
WO 9602551 A1 19960201 - MIDWEST RESEARCH INST [US]
US 5536655 A 19960716 - THOMAS STEVEN R [US], et al
WO 0070031 A1 20001123 - MIDWEST RESEARCH INST [US], et al
US 5246853 A 19930921 - CLARKSON KATHLEEN A [US], et al
US 5861271 A 19990119 - FOWLER TIMOTHY [US], et al
WO 9206209 A1 19920416 - GENENCOR INT [US]
US 5475101 A 19951212 - WARD MICHAEL [US], et al
WO 9428117 A1 19941208 - ALKO AB OY [FI], et al
EP 0244234 A2 19871104 - ALKO LTD [FI]
US 6255115 B1 20010703 - BEIJERSBERGEN ALIDA GODELIEVE [NL], et al
US 5874276 A 19990223 - FOWLER TIMOTHY [US], et al
US 2003096342 A1 20030522 - ADNEY WILLIAM S [US], et al
US 2003108988 A1 20030612 - DING SHI-YOU [US], et al
SINGLETON ET AL.: "DICTIONARY OF MICROBIOLOGY AND MOLECULAR BIOLOGY, 2D ED.", 1994, JOHN WILEY AND SONS
HALE; MARHAM: "THE HARPER COLLINS DICTIONARY OF BIOLOGY", 1991, HARPER PERENNIAL
SAMBROOK ET AL.: "MOLECULAR CLONING: A LABORATORY MANUAL(Second and Third Editions),", 1989, COLD SPRING HARBOR PRESS
AUSUBEL FM ET AL.: "Current Protocols in Molecular Biology", 1993, JOHN WILEY & SONS
SHOEMAKER, S. ET AL., BIO/TECHNOLOGY, vol. 1, 1983, pages 691 - 696
TEERI, T. ET AL., GENE, vol. 51, 1987, pages 43 - 52
PENTTILA, M. ET AL., GENE, vol. 45, 1986, pages 253 - 263
SALOHEIMO, M. ET AL., GENE, vol. 63, 1988, pages 11 - 22
OKADA, M. ET AL., APPL. ENVIRON. MICROBIOL., vol. 64, 1988, pages 555 - 563
SALOHEIMO, M. ET AL., EUR. J. BIOCHEM., vol. 249, 1997, pages 584 - 591
SALOHEIMO, A. ET AL., MOLECULAR MICROBIOLOGY, vol. 13, 1994, pages 219 - 228
SMITH; WATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482
NEEDLEMAN; WUNSCH, J. MOL. BIOL., vol. 48, 1970, pages 443
PEARSON; LIPMAN, PROC. NAT'L ACAD. SCI. USA, vol. 85, 1988, pages 2444
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
HENIKOFF; HENIKOFF, PROC. NATL. ACAD. SCI. USA, vol. 89, 1989, pages 10915
FINKELSTEIN ET AL.: "BIOTECHNOLOGY OF FILAMENTOUS FUNGI", 1992, BUTTERWORTH-HEINEMANN
KELLEY ET AL., EMBO J., vol. 4, 1985, pages 475 - 479
PENTTILA ET AL., GENE, vol. 61, 1987, pages 155 - 164
VAN DEN HONDEL, C.A.M.J.J. ET AL.: "More Gene Manipulations in Fungi", 1991, ACADEMIC PRESS, pages: 396 - 428
SHEIR-NEISS ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 20, 1984, pages 46 - 53
GOEDEGEBUUR ET AL., CURR. GENET, vol. 41, 2002, pages 89 - 98
WARD, M ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 39, 1993, pages 738 - 743
POURQUIE, J. ET AL.: "BIOCHEMISTRY AND GENETICS OF CELLULOSE DEGRADATION", 1988, ACADEMIC PRESS, pages: 71 - 86
OMEN, M. ET AL., APPL. ENVIRON. MICROBIOL., vol. 63, 1997, pages 1298 - 1306
LORITO; HAYES; DIPIETRO; HARMAN, CURR. GENET., vol. 24, 1993, pages 349 - 356
GOLDMAN, VANMONTAGU; HERRERA-ESTRELLA, CURR. GENET., vol. 17, 1990, pages 169 - 174
PENTTILA; NEVALAINEN; RATTO; SALMINEN; KNOWLES, GENE, vol. 61, 1987, pages 155 - 164
YELTON; HAMER; TIMBERLAKE, PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 1470 - 1474
BAJAR; PODILA; KOLATTUKUDY, PROC. NATL. ACAD. SCI. USA, vol. 88, 1991, pages 8202 - 8212
HOPWOOD ET AL.: "Genetic manipulation of streptomyces: a laboratory manual", 1985, THE JOHN INNES FOUNDATION
BRIGIDI; DEROSSI; BERTARINI; RICCARDI; MATTEUZZI, FEMS MICROBIOL. LETT., vol. 55, 1990, pages 135 - 138
CAMPBELL ET AL., CURR. GENET., vol. 16, 1989, pages 53 - 56
PENTTILA, M. ET AL., GENE, vol. 63, 1988, pages 11 - 22
PENTTILA, M. ET AL., GENE, vol. 61, 1987, pages 155 - 164
BECKER ET AL., BIOCHEM J., vol. 356, 2001, pages 19 - 30
MITSUISHI ET AL., FEBS, vol. 275, 1990, pages 135 - 138
SRISODSUK ET AL., J. BIOTECH., vol. 57, 1997, pages 49 - 57
NIDETZKY; CLAEYSSENS, BIOTECH. BIOENG., vol. 44, 1994, pages 961 - 966
VAN TILBEURGH ET AL., FEBS LETT., vol. 16, 1984, pages 215
GOYAL ET AL., BIORESOURCE TECHNOL., vol. 36, 1991, pages 37 - 50
FLIESS ET AL., EUR. J. APPL. MICROBIOL. BIOTECHNOL., vol. 17, 1983, pages 314 - 318
BHIKHABHAI ET AL., J. APPL. BIOCHEM., vol. 6, 1984, pages 336 - 345
ELLOUZ ET AL., CHROMATOGRAPHY, vol. 396, 1987, pages 307 - 317
MEDVE ET AL., J. CHROMATOGRAPHY A, vol. 808, 1998, pages 153 - 165
TOMAZ; QUEIROZ, J. CHROMATOGRAPHY A, vol. 865, 1999, pages 123 - 128
BRUMBAUER ET AL., BIOSEPARATION, vol. 7, 1999, pages 287 - 295
DEUTSCHER, METHODS IN ENZYMOLOGY, vol. 182, no. 57, 1990, pages 779
SCOPES, METHODS ENZYMOL., vol. 90, 1982, pages 479 - 91
HU ET AL., MOL CELL BIOL., vol. 11, no. 11, 1991, pages 5792 - 5799
PENTTILA ET AL., GENE, vol. 61, 1987, pages 155 - 164
FOREMAN ET AL., J. BIOL. CHEM, vol. 278, 2003, pages 31988 - 31997
DAVIS ET AL., METHODS IN ENZYMOLOGY, vol. 17A, 1970, pages 79 - 143
DAVIS; ROWLAND: "NEUROSPORA, CONTRIBUTIONS OF A MODEL ORGANISM", 2000, OXFORD UNIVERSITY PRESS
SCHELL, D. ET AL., J. APPL. BIOCHEM. BIOTECHNOL., vol. 105, 2003, pages 69 - 86
SMITH P. K. ET AL., BIOCHEM., vol. 150, 1985, pages 76 - 85
BAKER ET AL., APPL. BIOCHEM. BIOTECHNOL., vol. 70-72, 1998, pages 395 - 403
HAZELL, B. W. ET AL., LETT. APPL. MICROBIOL., vol. 30, 2000, pages 282 - 286
HAZELL B. ET AL., LETT. APPL. MICROBIOL., vol. 30, 2000, pages 282 - 286
ZHANG, S. ET AL., BIOCHEM., vol. 34, 1995, pages 3386 - 3395
COLLMER; WILSON, BIO/TECHNOL., vol. 1, 1983, pages 594 - 601
ALTSCHUL, S. F. ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
ALTSCHUL, S. F. ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
ARO N; SALOHEIMO A; LIMEN M; PENTTILA M: "ACEII, a novel transcriptional activator involved in regulation of cellulase and xylanase genes of Trichoderma reesei", J BIOL CHEM., vol. 276, no. 26, 29 June 2001 (2001-06-29), pages 24309 - 14
AUBERT J.P. ET AL.: "Federation of European Microbiological Societies", 1988, ACADEMIC PRESS, article "Biochemistry and Genetics of Cellulose Degradation", pages: 11
AUSUBEL G. M. ET AL.: "CURRENT PROTOCOLS IN MOLECULAR BIOLOGY", 1993, JOHN WILEY & SONS
BAKER ET AL., APPL. BIOCHEM. AND BIOTECHNOL., vol. 45/46, 1994, pages 245 - 256
BHIKHABHAI, R. ET AL., J. APPL. BIOCHEM., vol. 6, 1984, pages 336
BOEL ET AL., EMBO J, vol. 3, 1984, pages 1581 - 1585
BRUMBAUER, A. ET AL., BIOSEPARATION, vol. 7, 1999, pages 287 - 295
COLLMER A.; D. B. WILSON, BIO/TECHNOL., vol. 1, 1983, pages 594 - 601
DEUTSCHER, M.P., METHODS ENZYMOL., vol. 182, 1990, pages 779 - 80
ELLOUZ, S. ET AL., J. CHROMATOGRAPHY, vol. 396, 1987, pages 307
FILHO ET AL., CAN. J. MICROBIOL., vol. 42, 1996, pages 1 - 5
FLIESS, A. ET AL., EUR. J. APPL. MICROBIOL. BIOTECHNOL., vol. 17, 1983, pages 314
GOEDEGEBUUR ET AL., CURR. GENET, vol. 41, 2002, pages 89 - 98
GOYAL, A. ET AL., BIORESOURCE TECHNOL., vol. 36, 1991, pages 37
HERR ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 5, 1978, pages 29 - 36
HU ET AL., MOL. CELL. BIOL., vol. 11, 1991, pages 5792 - 9
JEEVES ET AL., BIOTECHNOL. GENET. ENG. REV., vol. 9, 1991, pages 327 - 369
KAWAGUCHI, T ET AL., GENE, vol. 173, no. 2, 1996, pages 287 - 8
KNOWLES, J. ET AL., TIBTECH, vol. 5, 1987, pages 255 - 261
KRISHNA, S. ET AL., BIORESOURCE TECH., vol. 77, 2001, pages 193 - 196
KUHLS K. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 93, no. 15, 1996, pages 7755 - 7760
KUMAR, A. ET AL., TEXTILE CHEMIST AND COLORIST, vol. 29, 1997, pages 37 - 42
MEDVE, J. ET AL., J. CHROMATOGRAPHY A, vol. 808, 1998, pages 153
MOHAGHEGHI, A. ET AL., INT. J. SYST. BACTERIOL., vol. 36, 1986, pages 435 - 443
NIEVES ET AL., APPL. BIOCHEM. AND BIOTECHNOL., vol. 51/52, 1995, pages 211 - 223
NUNBERG ET AL., MOL. CELL BIOL., vol. 4, 1984, pages 2306 - 2315
OHMIYA ET AL., BIOTECHNOL. GEN. ENGINEER. REV., vol. 14, 1997, pages 365 - 414
OOI ET AL., NUCLEIC ACID RES., vol. 18, 1990, pages 5884
PENTTILA ET AL., GENE, vol. 45, 1986, pages 253 - 263
PENTTILA ET AL., GENE, vol. 63, 1988, pages 103 - 112
PERE, J. ET AL., PROC. TAPPI PULPING CONF., vol. 27-31, 1996, pages 693 - 696
SAARILAHTI ET AL., GENE, vol. 90, 1990, pages 9 - 14
SAKAMOTO ET AL., CURR. GENET, vol. 27, 1995, pages 435 - 439
SALOHEIMO M ET AL., GENE, vol. 63, 1988, pages 11 - 22
SCHULEIN, METHODS ENZYMOL., vol. 160, no. 25, 1988, pages 234
SCOPES, METHODS ENZYMOL., vol. 90, 1982, pages 479 - 90
SHOEMAKER ET AL., BIOCHEM. BIOPHYS. ACAT., vol. 523, 1978, pages 133 - 146
SRISODSUK, M. ET AL., J. BIOL. CHEM., vol. 268, no. 28, 1993, pages 20756 - 20761
STRATHERN ET AL.,: "The Molecular Biology of the Yeast Saccharomyces", 1981, COLD SPRING HARBOR PRESS
SUURNAKKI, A. ET AL., CELLULOSE, vol. 7, 2000, pages 189 - 209
VAN TILBEURGH, H. ET AL., FEBS LETT., vol. 16, 1984, pages 215
TOMAZ, C.; QUEIROZ, J., J. CHROMATOGRAPHY A, vol. 865, 1999, pages 123 - 128
TOMME, P. ET AL., EUR. J. BIOCHEM., vol. 170, 1988, pages 575 - 581
VAN TILBEURGH, H. ET AL., FEBS LETT., vol. 204, 1986, pages 223 - 227
WARD, M. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 39, 1993, pages 738 - 743
WOOD, BIOCHEM. SOC. TRANS., vol. 13, 1985, pages 407 - 410
WOOD ET AL.: "METHODS IN ENZYMOLOGY", vol. 160, 1988, ACADEMIC PRESS, pages: 87

Citation (search report)

[Y] WO 9727306 A1 19970731 - ALKO GROUP LTD [FI], et al
[Y] WO 9831821 A2 19980723 - GENENCOR INT [US]
[Y] WO 9201797 A1 19920206 - ALKO AB OY [FI]
[Y] WARREN R A ET AL: "A bifunctional exoglucanase-endoglucanase fusion protein", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 61, no. 3, 1987, pages 421 - 427, XP002342343, ISSN: 0378-1119
[Y] KERÄNEN ET AL: "Production of recombinant proteins in the filamentous fungus Trichoderma reesei", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 6, no. 6, 1995, pages 534 - 537, XP002109010, ISSN: 0958-1669
[Y] BERGQUIST P ET AL: "Expression of xylanase enzymes from thermophilic microorganisms in fungal hosts", EXTREMOPHILES, SPRINGER VERLAG, TOKYO,, JP, vol. 6, no. 3, June 2002 (2002-06-01), pages 177 - 184, XP002327848, ISSN: 1431-0651
[Y] DE FARIA F P ET AL: "Expression and processing of a major xylanase (xyn2) from the thermophilic fungus Humicola grisea var. thermoidea in Trichoderma reesei", LETTERS IN APPLIED MICROBIOLOGY, OXFORD, GB, vol. 34, no. 2, 2002, pages 119 - 123, XP002342344
[Y] NYYSOENEN E ET AL: "PROTEIN PRODUCTION BY THE FILAMENTOUS FUNGUS TRICHODERMA REESEI: SECRETION OF ACTIVE ANTIBODY MOLECULES", CANADIAN JOURNAL OF BOTANY / JOURNAL CANADIEN DE BOTANIQUE, NATIONAL RESEARCH COUNCIL, OTTAWA, CA, vol. 70, no. SUPPL 1, January 1995 (1995-01-01), pages S885 - S890, XP000575039, ISSN: 0008-4026
[Y] NYYSSONEN E ET AL: "MULTIPLE ROLES OF THE CELLULASE CBHI IN ENHANCING PRODUCTION OF FUSION ANTIBODIES BY THE FILAMENTOUS FUNGUS TRICHODERMA REESEI", CURRENT GENETICS, NEW YORK, NY, US, vol. 28, no. 1, 1995, pages 71 - 79, XP008039837, ISSN: 0172-8083
[AD] BAKER J O ET AL: "A NEW THERMOSTABLE ENDOGLUCANASE, ACIDOTHERMUS CELLULOLYTICUS E1. SYNERGISM WITH TRICHODERMA REESEI CBH I AND COMPARISON TO THERMOMONOSPORA FUSCA E5", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, CLIFTON, NJ, US, vol. 45/46, 1994, pages 245 - 256, XP000672157, ISSN: 0273-2289

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR

Designated extension state (EPC)

AL BA HR LV MK YU

DOCDB simple family (publication)

WO 2005093050 A2 20051006; WO 2005093050 A3 20060824; BR PI0509212 A 20070828; CA 2560588 A1 20051006; CN 1930294 A 20070314; EP 1735447 A2 20061227; EP 2354222 A1 20110810; JP 2007530054 A 20071101; JP 5114194 B2 20130109

DOCDB simple family (application)

US 2005010242 W 20050325; BR PI0509212 A 20050325; CA 2560588 A 20050325; CN 200580008190 A 20050325; EP 05744703 A 20050325; EP 10010133 A 20050325; JP 2007505255 A 20050325