EP 2368123 A1 20110928 - IN-PROCESS CONTROL IN A METHOD FOR PRODUCING EPO

Title (en)

IN-PROCESS CONTROL IN A METHOD FOR PRODUCING EPO

Title (de)

INPROZESSKONTROLLE IN EINEM VERFAHREN ZUR HERSTELLUNG VON EPO

Title (fr)

CONTRÔLE LORS DE LA FABRICATION, DANS UN PROCÉDÉ DE PRODUCTION D'EPO

Publication

EP 2368123 A1 20110928 (DE)

Application

EP 09764266 A 20091207

Priority

EP 2009066517 W 20091207
DE 102008054716 A 20081216

Abstract (en)

[origin: CA2747320A1] The invention relates to a method for determining the isoform composition of erythropoietin, comprising the following steps: a) isoelectrical focusing of a sample comprising erythropoietin in a gel over a pH range having a lower limit of 2.5 to 3.5 and an upper limit of 5 to 8, wherein the sample comprising erythropoietin originates from a culture supernatant of erythropoietin producing eukaryotic cells; b) transferring of the proteins comprised and separated in the gel to a membrane; c) verifying the erythropoietin bound to the membrane by specific antibodies; and to a method for in-process control of culture supernatants of erythropoietin producing eukaryotic cells during the fermentative production process.

IPC 8 full level

G01N 33/74 (2006.01)

CPC (source: EP US)

G01N 33/746 (2013.01 - EP US)

Citation (search report)

See references of WO 2010076125A1

Citation (examination)

WO 03080852 A1 20031002 - LEK TOVARNA FARMACEVTSKIH [SI], et al
WO 03045996 A1 20030605 - BIOCHEMIE GMBH [AT], et al

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

DOCDB simple family (publication)

DE 102008054716 A1 20100617; BR PI0923039 A2 20151215; CA 2747320 A1 20100708; CN 102317791 A 20120111; EP 2368123 A1 20110928; IL 213544 A0 20110731; JP 2012512406 A 20120531; SG 172203 A1 20110728; US 2012021441 A1 20120126; WO 2010076125 A1 20100708

DOCDB simple family (application)

DE 102008054716 A 20081216; BR PI0923039 A 20091207; CA 2747320 A 20091207; CN 200980156804 A 20091207; EP 09764266 A 20091207; EP 2009066517 W 20091207; IL 21354411 A 20110614; JP 2011541315 A 20091207; SG 2011044179 A 20091207; US 200913140190 A 20091207