Global Patent Index - EP 2372347 A1

EP 2372347 A1 20111005 - Method to increase the number of detectable photons during the imaging of a biological marker

Title (en)

Method to increase the number of detectable photons during the imaging of a biological marker

Title (de)

Verfahren zur Erhöhung der Anzahl detektierbarer Photonen während der Abbildung eines biologischen Markers

Title (fr)

Procédé pour augmenter le nombre de photons détectables pendant l'imagerie d'un marqueur biologique

Publication

EP 2372347 A1 20111005 (EN)

Application

EP 10290158 A 20100326

Priority

EP 10290158 A 20100326

Abstract (en)

The present invention relates a method to determine the presence of a photon producing biological marker in a cell, tissue or organism of interest. The method is based on Fluorescence by Unbound Excitation from Luminescence (FUEL) and comprises the steps of a) providing conditions suitable for the biological marker to produce at least one first photon by luminescence b) providing a FUEL probe pair-upper (FPP-U) disposed in proximity to the cell, tissue or organism, wherein the at least one first photon of step a) excites the FPP-U, which emits at least one second photon. The FPP-U may be selected from the group of quantum dots, carbon nanotubes, fluorescent proteins, diamond nanocrystals, and metalloporphyrins. This method is characterized in that said biological marker and said FPP-U are not bound and in that each of the at least one second photon (s) are of a longer wavelength than each of the at least one first photon (s).

IPC 8 full level

G01N 21/64 (2006.01)

CPC (source: EP KR US)

B82Y 15/00 (2013.01 - EP KR US); G01N 21/6428 (2013.01 - EP KR US); G01N 21/6486 (2013.01 - KR US); G01N 2201/06193 (2013.01 - EP KR US)

Citation (applicant)

  • CONTAG, C. H.; ROSS, B. D.: "It's not just about anatomy: in vivo bioluminescence imaging as an eyepiece into biology", J MAGN RESON IMAGING, vol. 16, no. 4, 2002, pages 3 78 - 87
  • TROY, T.; JEKIC-MCMULLEN, D.; SAMBUCETTI, L.; RICE, B.: "Quantitative comparison of the sensitivity of detection of fluorescent and bioluminescent reporters in animal models", MOLECULAR IMAGING : OFFICIAL JOURNAL OF THE SOCIETY FOR MOLECULAR IMAGING, vol. 3, no. 1, 2004, pages 9 - 23
  • HUTCHENS, M.; LUKER, G. D.: "Applications of bioluminescence imaging to the study of infectious diseases", CELL MICROBIOL, vol. 9, no. 10, 2007, pages 2315 - 22
  • WELSH, D. K.; KAY, S. A.: "Bioluminescence imaging in living organisms", CURR OPIN BIOTECHNOL, vol. 16, no. 1, 2005, pages 73 - 8
  • ROGERS, K. L.; PICAUD, S.; RONCALI, E.; BOISGARD, R.; COLASANTE, C.; STINNAKRE, J.; TAVITIAN, B.; BRULET, P.: "Non-invasive in vivo imaging of calcium signaling in mice", PLOS ONE, vol. 2, no. 10, 2007, pages E974
  • CURIE, T.; ROGERS, K. L.; COLASANTE, C.; BRÛLET, P.: "Red-shifted aequorin-based bioluminescent reporters for in vivo imaging of Ca2 signaling", MOLECULAR IMAGING, vol. 6, no. 1, 2007, pages 30 - 42
  • WU, C.; MINO, K.; AKIMOTO, H.; KAWABATA, M.; NAKAMURA, K.; OZAKI, M.; OHMIYA, Y.: "In vivo far-red luminescence imaging of a biomarker based on BRET from Cypridina bioluminescence to an organic dye", PROC NATL ACAD SCI USA, vol. 106, no. 37, 2009, pages 15599 - 603
  • SO, M.; XU, C.; LOENING, A.; GAMBHIR, S.; RAO, J.: "Self-illuminating quantum dot conjugates for in vivo imaging", NATURE BIOTECHNOLOGY, vol. 24, no. 3, 2006, pages 339 - 343
  • LOENING, A. M.; DRAGULESCU-ANDRASI, A.; GAMBHIR, S. S.: "A red-shifted Renilla luciferase for transient reporter-gene expression", NAT METH, vol. 7, no. 1, 2010, pages 5 - 6
  • COLIN, M.; MORITZ, S.; SCHNEIDER, H.; CAPEAU, J.; COUTELLE, C.; BRAHIMI-HORN, M. C.: "Haemoglobin interferes with the ex vivo luciferase luminescence assay: consequence for detection of luciferase reporter gene expression in vivo", GENE THER, vol. 7, no. 15, 2000, pages 1333 - 6
  • BRANCHINI, B. R.; ABLAMSKY, D. M.; DAVIS, A. L.; SOUTHWORTH, T. L.; BUTLER, B.; FAN, F.; JATHOUL, A. P.; PULE, M. A.: "Red-emitting luciferases for bioluminescence reporter and imaging applications", ANALYTICAL BIOCHEMISTRY, vol. 396, no. 2, 2010, pages 290 - 7
  • WU, P.; BRAND, L.: "Resonance energy transfer: methods and applications", ANALYTICAL BIOCHEMISTRY, vol. 218, no. 1, 1994, pages 1 - 13
  • PFLEGER, K. D.; EIDNE, K. A.: "New technologies: bioluminescence resonance energy transfer (BRET) for the detection of real time interactions involving G-protein coupled receptors", PITUITARY, vol. 6, no. 3, 2003, pages 141 - 51
  • TAKANISHI, C. L.; BYKOVA, E. A.; CHENG, W.; ZHENG, J.: "GFP-based FRET analysis in live cells", BRAIN RES, vol. 1091, no. 1, 2006, pages 132 - 9
  • DACRES, H.; WANG, J.; DUMANCIC, M. M.; TROWELL, S. C.: "Experimental determination of the Forster distance for two commonly used bioluminescent resonance energy transfer pairs", ANAL CHEM, vol. 82, no. 1, 2010, pages 432 - 5
  • MICHALET, X.; PINAUD, F. F.; BENTOLILA, L. A.; TSAY, J. M.; DOOSE, S.; LI, J. J.; SUNDARESAN, G.; WU, A. M.; GAMBHIR, S. S.; WEISS: "Quantum dots for live cells, in vivo imaging, and diagnostics", SCIENCE, vol. 307, no. 5709, 2005, pages 538 - 44
  • CLAPP, A. R.; MEDINTZ, I. L.; MAURO, J. M.; FISHER, B. R.; BAWENDI, M. G.; MATTOUSSI, H.: "Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors", JAM CHEM SOC, vol. 126, no. 1, 2004, pages 301 - 10
  • BANG, J. H.; KAMAT, P. V.: "Quantum dot sensitized solar cells. A tale of two semiconductor nanocrystals: CdSe and CdTe", ACS NANO, vol. 3, no. 6, 2009, pages 1467 - 76
  • REISS, P.; BLEUSE, J.; PRON, A.: "Highly luminescent CdSe/ZnSe core/shell nanocrystals of low size dispersion", IN NANO LETT, vol. 2, 2002, pages 781 - 784
  • CHOI, K. H.; SCHWEIZER, H. P.: "mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa", NAT. PROTOC., vol. 1, no. 1, 2006, pages 170 - 178
  • ROGERS, K. L.; MARTIN, J. R.; RENAUD, 0.; KARPLUS, E.; NICOLA, M. A.; NGUYEN, M.; PICAUD, S.; SHORTE, S.; BRULET, P.: "Electron-multiplying charge-coupled detector- based bioluminescence recording of single-cell Ca2+", J BIOMED OPT, vol. 13, no. 3, 2008, pages 031211
  • SHU, X.; ROYANT, A.; LIN, M. Z.; AGUILERA, T. A.; LEV-RAM, V.; STEINBACH, P.; TSIEN, R.: "Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome", SCIENCE, vol. 324, no. 5928, 2009, pages 804 - 7
  • YANG, R.; CHANG, L.; WU, J.; TSAI, M.; WANG, H.; KUO, Y.; YEH, T.; YANG, C.; LIN, P.: "Persistent Tissue Kinetics and Redistribution of Nanoparticles, Quantum Dot 705, in Mice: ICP-MS Quantitative Assessment", ENVIRON. HEALTH PERSPECT., 2007, pages 5
  • HARDMAN, R.: "A toxicologic review of quantum dots: toxicity depends on physicochemical and environmental factors", ENVIRON. HEALTH PERSPECT., vol. 114, no. 2, 2006, pages 165 - 72
  • SMITH, A. M.; DUAN, H.; MOHS, A. M.; NIE, S.: "Bioconjugated quantum dots for in vivo molecular and cellular imaging", ADV DRUG DELIV REV, vol. 60, no. 11, 2008, pages 1226 - 40
  • TSAY, J. M.; MICHALET, X.: "New light on quantum dot cytotoxicity", CHEMISTRY & BIOLOGY, vol. 12, no. 11, 2005, pages 1159 - 61
  • CAI, W.; SHIN, D. W.; CHEN, K.; GHEYSENS, O.; CAO, Q.; WANG, S. X.; GAMBHIR, S.; CHEN, X.: "Peptide-labeled near-infrared quantum dots for imaging tumor vasculature in living subjects", NANO LETT, vol. 6, no. 4, 2006, pages 669 - 76
  • WELSHER, K.; LIU, Z.; SHERLOCK, S. P.; ROBINSON, J. T.; CHEN, Z.; DARANCIANG, D.; DAI, H.: "A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice", NAT NANOTECHNOL, vol. 4, no. 11, 2009, pages 773 - 80
  • KRUEGER, A.: "New carbon materials: biological applications of functionalized nanodiamond materials", CHEMISTRY, vol. 14, no. 5, 2008, pages 1382 - 90
  • YU, S.-J.; KANG, M.-W.; CHANG, H.-C.; CHEN, K.-M.; YU, Y.-C.: "Bright fluorescent nanodiamonds: no photobleaching and low cytotoxicity", J AM CHEM SOC, vol. 127, no. 50, 2005, pages 17604 - 5
  • SONG, B.; WANG, G.; TAN, M.; YUAN, J.: "A europium(III) complex as an efficient singlet oxygen luminescence probe", JAM CHEM SOC, vol. 128, no. 41, 2006, pages 13442 - 50
  • STROVAS, T. J.; DRAGAVON, J. M.; HANKINS, T. J.; CALLIS, J. B.; BURGESS, L. W.; LIDSTROM, M.: "Measurement of respiration rates of Methylobacterium extorquens AM cultures by use of a phosphorescence-based sensor", APPL ENVIRON MICROBIOL, vol. 72, no. 2, 2006, pages 1692 - 5
  • SOINI, A. E.; SEVEUS, L.; MELTOLA, N. J.; PAPKOVSKY, D. B.; SOINI, E.: "Phosphorescent metalloporphyrins as labels in time-resolved luminescence microscopy: effect of mounting on emission intensity", MICROSC RES TECH, vol. 58, no. 2, 2002, pages 125 - 31

Citation (search report)

  • [A] BAKALOVA RUMIANA ET AL: "Quantum dots versus organic fluorophores in fluorescent deep-tissue imaging--merits and demerits", GENERAL PHYSIOLOGY AND BIOPHYSICS, BRATISLAVA, CZ, vol. 27, no. 4, 1 December 2008 (2008-12-01), pages 231 - 242, XP009137950, ISSN: 0231-5882
  • [A] NIKOLAOS C DELIOLANIS ET AL: "Performance of the red-shifted fluorescent proteins in deep-tissue molecular imaging applications", JOURNAL OF BIOMEDICAL OPTICS, SPIE - INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING, US LNKD- DOI:10.1117/1.2967184, vol. 13, no. 4, 1 July 2008 (2008-07-01), pages 44008 - 1, XP007914604, ISSN: 1083-3668
  • [A] JIANG ET AL: "Assessing Near-Infrared Quantum Dots for Deep Tissue, Organ, and Animal Imaging Applications", JOURNAL OF THE ASSOCIATION FOR LABORATORY AUTOMATION, ELSEVIER LNKD- DOI:10.1016/J.JALA.2007.09.002, vol. 13, no. 1, 4 January 2008 (2008-01-04), pages 6 - 12, XP022409931, ISSN: 1535-5535
  • [A] CARRIE L AMIOT ET AL: "Near-Infrared Fluorescent Materials for Sensing of Biological Targets", SENSORS, MDPI, BASEL, SU LNKD- DOI:10.3390/S8053082, vol. 8, 1 January 2008 (2008-01-01), pages 3082 - 3105, XP007914605, ISSN: 1424-8220, [retrieved on 20080508]
  • [A] WANG Y ET AL: "Bioapplication of nanosemiconductors", MATERIALS TODAY, ELSEVIER SCIENCE, KIDLINGTON, GB, vol. 8, no. 5, 1 May 2005 (2005-05-01), pages 20 - 31, XP025370710, ISSN: 1369-7021, [retrieved on 20050501]

Citation (examination)

DUSICA MAYSINGER ET AL: "Real-Time Imaging of Astrocyte Response to Quantum Dots:  In Vivo Screening Model System for Biocompatibility of Nanoparticles", NANO LETTERS, vol. 7, no. 8, 1 August 2007 (2007-08-01), pages 2513 - 2520, XP055116367, ISSN: 1530-6984, DOI: 10.1021/nl071611t

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

Designated extension state (EPC)

AL BA ME RS

DOCDB simple family (publication)

EP 2372347 A1 20111005; AU 2011231169 A1 20121018; AU 2011231169 B2 20150827; BR 112012024477 A2 20160531; BR 112012024477 B1 20200317; BR 112012024477 B8 20200331; CA 2800316 A1 20110929; CA 2800316 C 20190226; CN 102869983 A 20130109; CN 102869983 B 20150819; EP 2553431 A1 20130206; EP 2553431 B1 20170510; ES 2637026 T3 20171010; IL 222093 B 20180131; JP 2013527911 A 20130704; JP 5744170 B2 20150701; KR 101752026 B1 20170628; KR 20130067258 A 20130621; US 2013115647 A1 20130509; US 9329132 B2 20160503; WO 2011117847 A1 20110929; WO 2011117847 A9 20111215

DOCDB simple family (application)

EP 10290158 A 20100326; AU 2011231169 A 20110325; BR 112012024477 A 20110325; CA 2800316 A 20110325; CN 201180021806 A 20110325; EP 11722878 A 20110325; ES 11722878 T 20110325; IB 2011051282 W 20110325; IL 22209312 A 20120923; JP 2013500645 A 20110325; KR 20127028128 A 20110325; US 201113637551 A 20110325