EP 2374879 A2 20111012 - Promoter and plasmid system for genetic engineering

Title (en)

Promoter and plasmid system for genetic engineering

Title (de)

Promoter und Plasmidsystem für Gentechnologie

Title (fr)

Promoteur et système de plasmide pour génie génétique

Publication

EP 2374879 A2 20111012 (EN)

Application

EP 10193565 A 20030422

Priority

EP 03721832 A 20030422
US 37493102 P 20020422

Abstract (en)

This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.

IPC 8 full level

C12N 1/21 (2006.01); C12N 9/90 (2006.01); C12N 15/09 (2006.01); C12N 15/61 (2006.01); C12N 15/70 (2006.01); C12P 7/18 (2006.01)

CPC (source: EP KR US)

C07K 14/36 (2013.01 - EP US); C12N 9/90 (2013.01 - EP US); C12N 15/11 (2013.01 - KR); C12N 15/52 (2013.01 - KR); C12N 15/63 (2013.01 - KR); C12N 15/70 (2013.01 - EP US); C12N 15/76 (2013.01 - EP US); C12P 7/18 (2013.01 - EP US)

Citation (applicant)

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Designated contracting state (EPC)

BE DE FR GB NL

DOCDB simple family (publication)

WO 03089621 A2 20031030; WO 03089621 A3 20040415; WO 03089621 A8 20041229; AU 2003225123 A1 20031103; AU 2003225123 A8 20031103; CA 2481414 A1 20031030; CA 2481414 C 20130409; CN 100366730 C 20080206; CN 1646680 A 20050727; EP 1499709 A2 20050126; EP 1499709 A4 20060802; EP 1499709 B1 20120104; EP 2374879 A2 20111012; EP 2374879 A3 20120111; JP 2005523020 A 20050804; JP 2009279007 A 20091203; JP 2010268807 A 20101202; JP 5291673 B2 20130918; KR 20040104581 A 20041210; MX PA04010366 A 20050217; US 2004235099 A1 20041125; US 2005147968 A1 20050707; US 2007065867 A1 20070322; US 2009156430 A1 20090618; US 2011046014 A1 20110224; US 7132527 B2 20061107; US 7510869 B2 20090331; US 8168608 B2 20120501; US 8192959 B2 20120605

DOCDB simple family (application)

US 0312566 W 20030422; AU 2003225123 A 20030422; CA 2481414 A 20030422; CN 03809122 A 20030422; EP 03721832 A 20030422; EP 10193565 A 20030422; JP 2003586334 A 20030422; JP 2009193493 A 20090824; JP 2010156143 A 20100708; KR 20047016903 A 20030422; MX PA04010366 A 20030422; US 37086409 A 20090213; US 42058703 A 20030422; US 54181006 A 20061002; US 73954203 A 20031218; US 94022510 A 20101105