Global Patent Index - EP 2376625 A4

EP 2376625 A4 20121010 - SOMATIC CELL-DERIVED PLURIPOTENT CELLS AND METHODS OF USE THEREFOR

Title (en)

SOMATIC CELL-DERIVED PLURIPOTENT CELLS AND METHODS OF USE THEREFOR

Title (de)

PLURIPOTENTE ZELLEN AUS KÖRPERZELLEN UND IHRE VERWENDUNG

Title (fr)

CELLULES PLURIPOTENTES DÉRIVÉES DE CELLULES STOMATIQUES ET LEURS MÉTHODES D'UTILISATION

Publication

EP 2376625 A4 20121010 (EN)

Application

EP 09832539 A 20091210

Priority

  • US 2009067503 W 20091210
  • US 20142008 P 20081210

Abstract (en)

[origin: WO2010068758A1] Provided are methods for producing a reprogrammed fibroblast. The methods can include growing a plurality of fibroblasts in monolayer culture to confluency; and disrupting the monolayer culture to place at least a fraction of the plurality of fibroblasts into suspension culture under conditions sufficient to form one or more embryoid body-like spheres, wherein the one or more embryoid body-like spheres comprise one or more reprogrammed fibroblasts that express one or more markers not expressed by a fibroblast growing in the monolayer culture prior to the disrupting step. Also provided are reprogrammed fibroblasts produced by the disclosed methods, formulations that include reprogrammed fibroblasts, and methods for treating an injury to a tissue in a subject by administering to a subject in need thereof a composition of reprogrammed fibroblast cells in a pharmaceutically acceptable carrier.

IPC 8 full level

C12N 5/00 (2006.01); C12N 5/0735 (2010.01)

CPC (source: EP)

C12N 5/0696 (2013.01); C12N 2501/405 (2013.01); C12N 2506/13 (2013.01); C12N 2506/1307 (2013.01); C12N 2506/27 (2013.01); C12N 2510/00 (2013.01)

Citation (search report)

  • [XI] US 2008268054 A1 20081030 - BELL EUGENE [US], et al
  • [XI] KATJA SCHENKE-LAYLAND ET AL: "Reprogrammed Mouse Fibroblasts Differentiate into Cells of the Cardiovascular and Hematopoietic Lineages", STEM CELLS, ALPHAMED PRESS, DAYTON, OH, US, vol. 26, no. 6, 1 June 2008 (2008-06-01), pages 1537 - 1546, XP008142741, ISSN: 1066-5099, [retrieved on 20080501], DOI: 10.1634/STEMCELLS.2008-0033
  • [XI] KAZUTOSHI TAKAHASHI ET AL: "Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors", CELL, CELL PRESS, US, vol. 131, no. 5, 30 November 2007 (2007-11-30), pages 861 - 872, XP008153593, ISSN: 0092-8674, DOI: 10.1016/J.CELL.2007.11.019
  • [XP] LIU YONGQING ET AL: "Mouse Fibroblasts Lacking RB1 Function Form Spheres and Undergo Reprogramming to a Cancer Stem Cell Phenotype", CELL STEM CELL, vol. 4, no. 4, April 2009 (2009-04-01), pages 336 - 347, XP002682618, ISSN: 1934-5909
  • [AP] TE RIELE HEIN: "Recreating Stem Cells: A Novel Entrance to the Fountain of Youth", CELL STEM CELL, vol. 4, no. 4, April 2009 (2009-04-01), pages 279 - 280, XP002682619, ISSN: 1934-5909
  • [A] SAGE JULIEN ET AL: "Targeted disruption of the three Rb-related genes leads to loss of G1 control and immortalization", GENES AND DEVELOPMENT, vol. 14, no. 23, 1 December 2000 (2000-12-01), pages 3037 - 3050, XP002682620, ISSN: 0890-9369
  • See references of WO 2010068758A1

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

Designated extension state (EPC)

AL BA RS

DOCDB simple family (publication)

WO 2010068758 A1 20100617; AU 2009324641 A1 20110728; EP 2376625 A1 20111019; EP 2376625 A4 20121010

DOCDB simple family (application)

US 2009067503 W 20091210; AU 2009324641 A 20091210; EP 09832539 A 20091210