Global Patent Index - EP 2388316 A3

EP 2388316 A3 20120314 - Aldolases, nucleic acids encoding them and methods for making and using them

Title (en)

Aldolases, nucleic acids encoding them and methods for making and using them

Title (de)

Adolasen, Nukleinsäuren, die diese codieren, und Verfahren zu deren Herstellung und Verwendung

Title (fr)

Aldolases, acides nucléiques les codant et leurs procédés de fabrication et d'utilisation

Publication

EP 2388316 A3 20120314 (EN)

Application

EP 11167408 A 20070307

Priority

  • EP 07758102 A 20070307
  • US 78051506 P 20060307

Abstract (en)

[origin: WO2007103989A2] This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S, R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S, R configurations, and salts thereof.

IPC 8 full level

C12N 9/10 (2006.01); A23L 27/30 (2016.01); A23L 29/00 (2016.01); C12N 9/88 (2006.01); C12N 15/82 (2006.01)

CPC (source: EP KR US)

A61K 38/00 (2013.01 - KR); A61P 31/04 (2017.12 - EP); A61P 35/00 (2017.12 - EP); A61P 43/00 (2017.12 - EP); C12N 9/1022 (2013.01 - EP KR US); C12N 9/88 (2013.01 - EP KR US); C12N 15/52 (2013.01 - KR); C12N 15/8243 (2013.01 - EP KR US); C12P 17/10 (2013.01 - EP KR US); A01K 2217/05 (2013.01 - EP KR US); A61K 38/00 (2013.01 - EP US); C07K 2317/92 (2013.01 - EP US); C12Y 401/03016 (2013.01 - EP US); C12Y 401/03017 (2013.01 - EP US); Y02E 50/10 (2013.01 - KR US); Y02P 20/52 (2015.11 - KR)

Citation (search report)

  • [A] US 2005282260 A1 20051222 - HICKS PAULA M [US], et al
  • [I] MARUYAMA K ET AL: "Cloning, sequencing and expression of the gene encoding 4-hydroxy-4-methyl-2-oxoglutarate aldolase from pseudomonas ochraceae NGJ1", BIOSCIENCE BIOTECHNOLOGY BIOCHEMISTRY, JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY, TOKYO, JAPAN, vol. 65, no. 12, 23 December 2001 (2001-12-23), pages 2701 - 2709, XP002965270, ISSN: 0916-8451, DOI: 10.1271/BBB.65.2701 & DATABASE UniProt [online] 1 June 2001 (2001-06-01), "SubName: Full=Nodulation glucosamine synthase NodM;", XP002659207, retrieved from EBI accession no. UNIPROT:Q9AQ10 Database accession no. Q9AQ10
  • [A] DATABASE UniProt [online] 1 June 2002 (2002-06-01), "SubName: Full=4-carboxy-4-hydroxy-2-oxoadipate aldolase;", XP002659208, retrieved from EBI accession no. UNIPROT:Q8RQW0 Database accession no. Q8RQW0
  • [A] DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1990, PATIL R V ET AL: "CLONING AND EXPRESSION OF 2 KETO-4-HYDROXYGLUTARATE KHG ALDOLASE OF ESCHERICHIA-COLI", XP009152308, Database accession no. PREV199039041255

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

Designated extension state (EPC)

AL BA HR MK RS

DOCDB simple family (publication)

WO 2007103989 A2 20070913; WO 2007103989 A8 20080228; WO 2007103989 A8 20090723; WO 2007103989 A9 20071129; AT E548450 T1 20120315; AU 2007223086 A1 20070913; AU 2007223086 B2 20130926; BR 122017003017 B1 20181023; BR PI0708614 A2 20110607; CA 2645225 A1 20070913; CN 101443446 A 20090527; CN 101443446 B 20130821; CN 102690833 A 20120926; CN 102690833 B 20150909; CN 105063072 A 20151118; EA 019971 B1 20140730; EA 029538 B1 20180430; EA 200870329 A1 20090630; EA 201301267 A1 20141128; EP 1999256 A2 20081210; EP 1999256 A4 20090603; EP 1999256 B1 20120307; EP 2385108 A1 20111109; EP 2385108 B1 20161123; EP 2388316 A2 20111123; EP 2388316 A3 20120314; EP 3153580 A2 20170412; EP 3153580 A3 20170419; ES 2383767 T3 20120626; JP 2009531025 A 20090903; JP 2013063068 A 20130411; JP 2013066469 A 20130418; JP 5733777 B2 20150610; JP 5793486 B2 20151014; JP 5793487 B2 20151014; KR 101508249 B1 20150410; KR 101588539 B1 20160125; KR 101622894 B1 20160519; KR 101744157 B1 20170608; KR 20090004918 A 20090112; KR 20140023424 A 20140226; KR 20150020722 A 20150226; KR 20160058194 A 20160524; MX 2008011442 A 20081118; US 2010095390 A1 20100415; US 2014053287 A1 20140220; US 2016251643 A1 20160901; US 8546118 B2 20131001; US 9290755 B2 20160322; US 9719079 B2 20170801

DOCDB simple family (application)

US 2007063515 W 20070307; AT 07758102 T 20070307; AU 2007223086 A 20070307; BR 122017003017 A 20070307; BR PI0708614 A 20070307; CA 2645225 A 20070307; CN 200780016408 A 20070307; CN 201210175110 A 20070307; CN 201510514805 A 20070307; EA 200870329 A 20070307; EA 201301267 A 20070307; EP 07758102 A 20070307; EP 11167408 A 20070307; EP 11167410 A 20070307; EP 16199751 A 20070307; ES 07758102 T 20070307; JP 2008558521 A 20070307; JP 2012225071 A 20121010; JP 2012225075 A 20121010; KR 20087024447 A 20070307; KR 20147000809 A 20070307; KR 20157002469 A 20070307; KR 20167012333 A 20070307; MX 2008011442 A 20070307; US 201313966880 A 20130814; US 201615009414 A 20160128; US 28105207 A 20070307