EP 2391389 A1 20111207 - NON-VIRAL TRANSFECTION AGENT
Title (en)
NON-VIRAL TRANSFECTION AGENT
Title (de)
NICHT-VIRALES TRANSFEKTIONSMITTEL
Title (fr)
VECTEUR DE TRANSFECTION NON VIRAL
Publication
Application
Priority
- EP 2010051081 W 20100129
- DE 102009006606 A 20090129
Abstract (en)
[origin: WO2010086406A1] The invention relates to a non-viral transfection agent comprising polymer/nucleic acid complexes and nanofibers, wherein the polymer/nucleic acid complexes are composed of at least one nucleic acid and at least one cationic polymer. The nanofibers carry the polymer/nucleic acid complexes, wherein the non-viral transfection agent is advantageously produced by means of electrospinning. The cationic polymer is favorably a polyimine or polyethyleneimine and can be modified with one or more hydrophilic polymers coupled thereto. It can also be advantageous to couple the cationic polymer with one or more carbohydrates and/or with a receptor-specific ligand. The nucleic acid is a DNA or an RNA, or a DNA or RNA derivative, advantageously a therapeutically active nucleic acid. The nanofibers are composed of biodegradable, biocompatible polymers. The nanofibers or the entire transfection agent can be provided with a polymer coating. A method for producing a non-viral transfection agent comprises the following steps: providing the polymer/nucleic acid complex, producing a spinning solution containing the polymer/nucleic acid complexes, and electrospinning.
IPC 8 full level
A61K 48/00 (2006.01)
CPC (source: EP US)
A61K 47/6935 (2017.07 - EP US); A61K 47/6937 (2017.07 - EP US); A61K 48/0041 (2013.01 - EP US); B82Y 5/00 (2013.01 - EP US); C12N 15/111 (2013.01 - EP US); C12N 15/87 (2013.01 - EP US); C12N 2310/14 (2013.01 - EP US); C12N 2320/32 (2013.01 - EP US)
Citation (search report)
See references of WO 2010086406A1
Citation (examination)
WO 02100628 A1 20021219 - UNIV AKRON [US]
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
DOCDB simple family (publication)
WO 2010086406 A1 20100805; DE 102009006606 A1 20100805; EP 2391389 A1 20111207; US 2012083037 A1 20120405
DOCDB simple family (application)
EP 2010051081 W 20100129; DE 102009006606 A 20090129; EP 10709985 A 20100129; US 201013145756 A 20100129