EP 2406392 A1 20120118 - QUANTIFICATION OF NUCLEIC ACIDS
Title (en)
QUANTIFICATION OF NUCLEIC ACIDS
Title (de)
QUANTIFIZIERUNG VON NUKLEINSÄUREN
Title (fr)
QUANTIFICATION D'ACIDES NUCLÉIQUES
Publication
Application
Priority
- EP 2010052989 W 20100309
- DE 102009012039 A 20090310
Abstract (en)
[origin: WO2010103010A1] The invention relates to a method for the quantification of one or more nucleic acids in a sample. The method comprises the following steps: making a sample available which contains at least one nucleic acid to be quantified, adding an oligonucleotide probe to the sample, the oligonucleotide probe comprising a sequence which can specifically hybridize to the nucleic acid to be quantified or to a common sequence of the nucleic acids to be quantified, incubating the sample under conditions which allow the hybridization of the oligonucleotide probe to the nucleic acid(s) to be quantified, incubating the sample under conditions which allow the extension of hybridized probes, the nucleic acid(s) serving as a template in each case, removing the non-hybridized probes from the sample and quantifying the hybridized oligonucleotide probes to measure the quantity of the nucleic acid(s) to be quantified. The invention also relates to a kit for carrying out said method.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/6806 (2013.01 - EP US)
Citation (search report)
See references of WO 2010103010A1
Citation (examination)
US 2008108063 A1 20080508 - LUCERO MICHAEL [US], et al
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
DOCDB simple family (publication)
DE 102009012039 A1 20100916; EP 2406392 A1 20120118; US 2012107813 A1 20120503; US 9587267 B2 20170307; WO 2010103010 A1 20100916
DOCDB simple family (application)
DE 102009012039 A 20090310; EP 10707901 A 20100309; EP 2010052989 W 20100309; US 201013255696 A 20100309