EP 2417434 A1 20120215 - METHOD AND DEVICE FOR CARRYING OUT A QUANTITATIVE SPATIALLY-RESOLVED LOCAL AND DISTRIBUTION ANALYSIS OF CHEMICAL ELEMENTS AND IN SITU CHARACTERIZATION OF THE ABLATED SURFACE REGIONS
Title (en)
METHOD AND DEVICE FOR CARRYING OUT A QUANTITATIVE SPATIALLY-RESOLVED LOCAL AND DISTRIBUTION ANALYSIS OF CHEMICAL ELEMENTS AND IN SITU CHARACTERIZATION OF THE ABLATED SURFACE REGIONS
Title (de)
VERFAHREN UND VORRICHTUNG ZUR DURCHFÜHRUNG EINER QUANTITATIVEN ORTSAUFGELÖSTEN LOKAL- UND VERTEILUNGSANALYSE CHEMISCHER ELEMENTE UND IN-SITU CHARAKTERISIERUNG DE ABLATIERTEN OBERFLÄCHENREGIONEN
Title (fr)
PROCÉDÉ ET DISPOSITIF POUR L'EXÉCUTION D'UNE ANALYSE QUANTITATIVE LOCALE À RÉSOLUTION SPATIALE DE LA RÉPARTITION D'ÉLÉMENTS CHIMIQUES ET LA CARACTÉRISATION IN SITU DES ZONES DE SURFACE ENLEVÉES
Publication
Application
Priority
- DE 2010000295 W 20100317
- DE 102009016512 A 20090408
Abstract (en)
[origin: WO2010115394A1] A laser ablation chamber, suitable for use in a conventional laser microdissection unit (LMD), enables in combination with the LMD both a quantitative spatially-resolved nano-local and distribution analysis of element concentrations in a sample and a microscopic recording of the surface topography of said sample in the nanometer range. This can optionally be followed by further examinations without for this purpose the sample needing to be removed from a slide comprising the sample. For the examination, a region of the sample to be analyzed is selected with the aid of a microscope of an LMD. In the process, the sample is situated on the underside of a cover glass (slide), which at the same time is part of a laser ablation chamber that is mounted underneath the slide and within the LMD. Part of the sample is ablated and analyzed. Optionally, it is possible to cut out in a targeted fashion certain regions of the tissue in which metals were detected by means of the available LMD instruments for the purpose of further analysis and to collect these regions in sample containers that are attached under the slide after the laser ablation.
IPC 8 full level
G01N 1/28 (2006.01); G02B 21/34 (2006.01)
CPC (source: EP US)
G01N 1/2813 (2013.01 - EP US); G02B 21/34 (2013.01 - EP US); H01J 49/0409 (2013.01 - US); H01J 49/0463 (2013.01 - EP US); G01N 2001/045 (2013.01 - EP US); G01N 2001/2886 (2013.01 - EP US)
Citation (search report)
See references of WO 2010115394A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
DOCDB simple family (publication)
WO 2010115394 A1 20101014; DE 102009016512 A1 20101021; DE 102009016512 B4 20110512; EP 2417434 A1 20120215; JP 2012523548 A 20121004; US 2012074307 A1 20120329; US 2013234015 A1 20130912; US 8497470 B2 20130730; US 8735813 B2 20140527
DOCDB simple family (application)
DE 2010000295 W 20100317; DE 102009016512 A 20090408; EP 10715085 A 20100317; JP 2012503856 A 20100317; US 201013138689 A 20100317; US 201313849915 A 20130325