EP 2430192 A1 20120321 - METHOD FOR DETECTING PROKARYOTIC DNA FROM A FECES SAMPLE
Title (en)
METHOD FOR DETECTING PROKARYOTIC DNA FROM A FECES SAMPLE
Title (de)
VERFAHREN ZUM NACHWEIS PROKARYONTISCHER DNA AUS EINER FAECESPROBE
Title (fr)
METHODE DE DETECTION D'ADN PROCARYOTE EXTRAIT D'UN ECHANTILLON DE SELLES
Publication
Application
Priority
- FR 2010050824 W 20100430
- FR 0953207 A 20090514
Abstract (en)
[origin: WO2010130914A1] The present invention relates to a method for detecting and preferably for quantifying DNA and preferably including prokaryotic DNA extracted from a sample of the feces of a person, in particular for determining the molecular composition of the intestinal flora in the feces. The invention comprises controlling the quality of the DNA extraction by checking if a specific DNA of Methanobrevibacter smithii is detected, and quantifying said prokaryotic DNA specific to Archae Methanobrevibacter smithii, to the bacterial genus Lactobacillus, to the phylum Bacteroidetes and to the phylum Firmicutes, for diagnosing and/or following up the weight status of a person. The present invention provides a method for carrying out the extraction of prokaryotic DNA from feces.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP US)
C12Q 1/689 (2013.01 - EP US); C12Q 1/6851 (2013.01 - EP US)
Citation (search report)
See references of WO 2010130914A1
Designated contracting state (EPC)
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
DOCDB simple family (publication)
WO 2010130914 A1 20101118; EP 2430192 A1 20120321; FR 2945545 A1 20101119; FR 2945545 B1 20110805; US 2012252012 A1 20121004
DOCDB simple family (application)
FR 2010050824 W 20100430; EP 10727071 A 20100430; FR 0953207 A 20090514; US 201013320343 A 20100430