EP 2449125 A4 20130529 - COMBINED NUCLEIC ACID BLOCKING, EXTRACTION, AND DETECTION IN A SINGLE REACTION VESSEL
Title (en)
COMBINED NUCLEIC ACID BLOCKING, EXTRACTION, AND DETECTION IN A SINGLE REACTION VESSEL
Title (de)
KOMBINIERTE NUKLEINSÄUREBLOCKIERUNG, -EXTRAKTION UND -ERKENNUNG IN EINEM EINZELREAKTIONSGEFÄSS
Title (fr)
BLOCAGE, EXTRACTION ET DÉTECTION COMBINÉS D'ACIDE NUCLÉIQUE DANS UN RÉCEPTACLE DE RÉACTION UNIQUE
Publication
Application
Priority
- NZ 2010000137 W 20100702
- US 22291209 P 20090702
Abstract (en)
[origin: WO2011002319A2] The invention relates to a method that utilizes nucleic acid deactivating reagents for the deactivation of contaminating nucleic acids, and thermophilic proteinases for the extraction of nucleic acids in a closed-system to be used in tandem with methods for the amplification of target nucleic acids present in a sample. The combined method enables simplified, temperature-controlled devices to be used for accurate, streamlined testing at the point of care for a wide variety of applications in the medical, industrial, environmental, quality control, security and research fields.
IPC 8 full level
C12P 19/34 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/6806 (2013.01); C12Q 1/6848 (2013.01)
Citation (search report)
- [AP] WO 2010019898 A1 20100218 - ZYGEM CORP LTD [NZ], et al
- [A] MOSS D ET AL: "An easily automated, closed-tube forensic DNA extraction procedure using a thermostable proteinase", INTERNATIONAL JOURNAL OF LEGAL MEDICINE, SPRINGER VERLAG, DE, vol. 117, no. 6, 1 December 2003 (2003-12-01), pages 340 - 349, XP002476705, ISSN: 0937-9827, [retrieved on 20031023], DOI: 10.1007/S00414-003-0400-9
- See references of WO 2011002319A2
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
DOCDB simple family (publication)
WO 2011002319 A2 20110106; WO 2011002319 A3 20110414; EP 2449125 A2 20120509; EP 2449125 A4 20130529; JP 2012531907 A 20121213
DOCDB simple family (application)
NZ 2010000137 W 20100702; EP 10794424 A 20100702; JP 2012518502 A 20100702