Title (en)

Cell culture medium and process for protein expression, said medium and process comprising a PAM inhibitor

Title (de)

Zellkulturmedium und Verfahren zur Proteinexpression, besagtes Medium und Verfahren mit einem PAM-Inhibitor

Title (fr)

Milieu de culture cellulaire et procédé pour l'expression de protéines, ledit milieu et ledit procédé comprenant un inhibiteur de PAM

Publication

EP 2450375 A1 20120509 (EN)

Application

EP 10190532 A 20101109

Priority

EP 10190532 A 20101109

Abstract (en)

The present invention is related to a cell culture medium for the expression of a protein, which medium comprises a PAM inhibitor, or a physiological equivalent thereof, and to a cell culture process for the expression of a protein, in which process a PAM inhibitor, or a physiological equivalent thereof, is used ( Fig. 1 ).

IPC 8 full level

C07K 16/00 (2006.01); C12N 5/00 (2006.01)

CPC (source: EP KR US)

C07K 16/00 (2013.01 - EP US); C12N 5/00 (2013.01 - KR); C12N 5/0018 (2013.01 - EP US); C12N 5/0031 (2013.01 - KR); C12N 5/005 (2013.01 - KR); C12P 21/00 (2013.01 - US); C12N 2501/70 (2013.01 - EP US); C12N 2501/71 (2013.01 - US)

Citation (applicant)

• CHEW, G: "Substrate-Based Inhibitors ofPeptidylglycine Amidating Monooxygenase (PAM) as Anti-Proliferative Drugs for Cancer", MASTER THESIS, 2003
• BAUER ET AL.: "Anti-Inflammatory Effects of 4-Phenyl-3-butenoic Acid and 5-(Acetylamino)-4-oxo-6-phenyl-2-hexenoic Acid Methyl Ester, Potential Inhibitors of Neuropeptide Bioactivation", J PHARMACOL EXP THER, vol. 320, March 2007 (2007-03-01), pages 1171 - 1177

Citation (search report)

• [Y] WO 2007087253 A2 20070802 - UNIGENE LAB INC [US], et al
• [A] WO 2006084111 A2 20060810 - GLAXO GROUP LTD [GB], et al
• [Y] MAINS R E ET AL: "Inhibition of peptide amidation by disulfiram and diethyldithiocarbamate.", THE JOURNAL OF BIOLOGICAL CHEMISTRY 15 SEP 1986 LNKD- PUBMED:3017953, vol. 261, no. 26, 15 September 1986 (1986-09-15), pages 11938 - 11941, XP002631131, ISSN: 0021-9258
• [XY] SUNMAN JEFFREY A ET AL: "Reversal of the transformed phenotype and inhibition of peptidylglycine alpha-monooxygenase in Ras-transformed cells by 4-phenyl-3-butenoic acid.", MOLECULAR CARCINOGENESIS DEC 2004 LNKD- PUBMED:15468302, vol. 41, no. 4, December 2004 (2004-12-01), pages 231 - 246, XP002631132, ISSN: 0899-1987
• [XY] IWAI N ET AL: "Autocrine growth loops dependent on peptidyl alpha-amidating enzyme as targets for novel tumor cell growth inhibitors.", LUNG CANCER (AMSTERDAM, NETHERLANDS) MAR 1999 LNKD- PUBMED:10413197, vol. 23, no. 3, March 1999 (1999-03-01), pages 209 - 222, XP002631133, ISSN: 0169-5002

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

Designated extension state (EPC)

BA ME

DOCDB simple family (publication)

EP 2450375 A1 20120509; AU 2011328166 A1 20130530; AU 2011328166 B2 20170223; CA 2816394 A1 20120518; CN 103298830 A 20130911; CN 107629995 A 20180126; DK 2638064 T3 20211004; EP 2638064 A2 20130918; EP 2638064 B1 20210721; HU E055573 T2 20211228; JP 2013541963 A 20131121; JP 2017035084 A 20170216; JP 6298295 B2 20180320; KR 101905204 B1 20181005; KR 101991215 B1 20190930; KR 20130131355 A 20131203; KR 20180082643 A 20180718; PL 2638064 T3 20211220; SG 10201602507U A 20160530; SG 190182 A1 20130628; SI 2638064 T1 20211130; US 10106828 B2 20181023; US 2013337506 A1 20131219; US 2015267238 A1 20150924; US 9062337 B2 20150623; WO 2012062810 A2 20120518; WO 2012062810 A3 20120705

DOCDB simple family (application)

EP 10190532 A 20101109; AU 2011328166 A 20111109; CA 2816394 A 20111109; CN 201180053542 A 20111109; CN 201710966840 A 20111109; DK 11782121 T 20111109; EP 11782121 A 20111109; EP 2011069756 W 20111109; HU E11782121 A 20111109; JP 2013538186 A 20111109; JP 2016163954 A 20160824; KR 20137013920 A 20111109; KR 20187019845 A 20111109; PL 11782121 T 20111109; SG 10201602507U A 20111109; SG 2013034871 A 20111109; SI 201131994 T 20111109; US 201113883986 A 20111109; US 201514721679 A 20150526