Global Patent Index - EP 2450434 A3

EP 2450434 A3 20120808 - Process for the production of arachidonic acid and/or eicosapentaenoic acid

Title (en)

Process for the production of arachidonic acid and/or eicosapentaenoic acid

Title (de)

Verfahren zur Herstellung von Arachidonsäure und/oder Eicosapentaensäure

Title (fr)

Processus de production d'acide arachidonique et/ou d'acide eicosapentaénoique

Publication

EP 2450434 A3 20120808 (EN)

Application

EP 11185517 A 20061010

Previously filed application

PCT/EP2006/067223 20061010 WO

Priority

  • EP 06841262 A 20061010
  • GB 0520843 A 20051013

Abstract (en)

[origin: GB2431158A] A process for the production of arachidonic acid and/or eicosapentaenoic acid in plants through the co-expression of a W -12-/W -15-desaturase, W -9-elongase, W -8-desaturase and a W -5-desaturase and a process for the production of lipids or oils having an increased content of unsaturated fatty acids, in particular l -3 and l -6 fatty acids having at least two double bonds and a 18 or 20 carbon atom chain length. Preferably the arachidonic acid and eicosapentaenoic acid are produced in at least a 1:2 ratio. The invention furthermore relates to the production of a transgenic plants, preferably a transgenic crop plant, having an increased content of arachidonic acid and/or eicosapentaenoic acid. The invention additionally relates to specific nucleic acid sequences encoding for proteins with W -12-/W -15-desaturase-, W -9-elongase-, W -8-desaturase- or W -5-desaturase-activity, nucleic acid constructs, vectors and transgenic plants containing said nucleic acid sequences.

IPC 8 full level

C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 15/82 (2006.01)

CPC (source: EP GB US)

C12N 5/14 (2013.01 - GB); C12N 9/0083 (2013.01 - EP US); C12N 9/1029 (2013.01 - EP US); C12N 15/52 (2013.01 - GB); C12N 15/82 (2013.01 - GB); C12N 15/8247 (2013.01 - EP US)

Citation (search report)

  • [X] WO 2005047479 A2 20050526 - DU PONT [US], et al
  • [A] WO 2004057001 A2 20040708 - UNIV BRISTOL [GB], et al
  • [A] WO 2005012316 A2 20050210 - BASF PLANT SCIENCE GMBH [DE], et al
  • [A] WO 9933958 A2 19990708 - UNIV BRISTOL [GB], et al
  • [A] QI B ET AL: "Production of very long chain polyunsaturated omega-3 and omega-6 fatty acids in plants", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 22, no. 6, June 2004 (2004-06-01), pages 739 - 745, XP002348313, ISSN: 1087-0156
  • [A] NAPIER J A ET AL: "PROGRESS TOWARD THE PRODUCTION OF LONG-CHAIN POLYUNSATURATED FATTY ACIDS IN TRANSGENIC PLANTS", LIPIDS, CHAMPAIGN, IL, US, vol. 39, no. 11, November 2004 (2004-11-01), pages 1067 - 1075, XP008061222, ISSN: 0024-4201
  • [A] WALLIS J G ET AL: "The DELTA8-desaturase of Euglena gracilis: An alternate pathway for synthesis of 20-carbon polyunsaturated fatty acids", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, NEW YORK, US, US, vol. 365, no. 2, 15 May 1999 (1999-05-15), pages 307 - 316, XP002291433, ISSN: 0003-9861
  • [A] QI^A B ET AL: "Identification of a cDNA encoding a novel C18-DELTA<9> polyunsaturated fatty acid-specific elongating activity from the docosahexaenoic acid (DHA)-producing microalga, Isochrysis galbana<1>", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 510, no. 3, 16 January 2002 (2002-01-16), pages 159 - 165, XP004332633, ISSN: 0014-5793
  • [A] DATABASE EMBL [online] 2 October 2001 (2001-10-02), "Mucor circinelloides D12d mRNA for delta-12 fatty acid desaturase, complete cds.", XP002423730, retrieved from EBI accession no. EMBL:AB052087 Database accession no. AB052087
  • [T] SAYANOVA OLGA ET AL: "A bifunctional Delta 12, Delta 15-desaturase from Acanthamoeba castellanii directs the synthesis of highly unusual n-1 series unsaturated fatty acids", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 281, no. 48, December 2006 (2006-12-01), pages 36533 - 36541, XP002423728, ISSN: 0021-9258

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

DOCDB simple family (publication)

EP 2450434 A2 20120509; EP 2450434 A3 20120808; EP 2450434 B1 20160106; AT E546519 T1 20120315; AU 2006301280 A1 20070419; AU 2006301280 B2 20120315; AU 2006301280 C1 20120830; CA 2621214 A1 20070419; CA 2621214 C 20181204; CA 3021294 A1 20070419; CA 3021294 C 20210921; EP 1937805 A2 20080702; EP 1937805 B1 20120222; ES 2380052 T3 20120508; GB 0520843 D0 20051123; GB 2431158 A 20070418; PL 1937805 T3 20120731; US 2010043103 A1 20100218; US 2012011618 A1 20120112; US 2012017332 A1 20120119; US 8017839 B2 20110913; US 8258371 B2 20120904; US 8273958 B2 20120925; WO 2007042510 A2 20070419; WO 2007042510 A3 20070802

DOCDB simple family (application)

EP 11185517 A 20061010; AT 06841262 T 20061010; AU 2006301280 A 20061010; CA 2621214 A 20061010; CA 3021294 A 20061010; EP 06841262 A 20061010; EP 2006067223 W 20061010; ES 06841262 T 20061010; GB 0520843 A 20051013; PL 06841262 T 20061010; US 201113186030 A 20110719; US 201113186056 A 20110719; US 8330006 A 20061010