Global Patent Index - EP 2454381 A1

EP 2454381 A1 20120523 - A METHOD FOR AMPLIFICATION OF NUCLEIC ACIDS

Title (en)

A METHOD FOR AMPLIFICATION OF NUCLEIC ACIDS

Title (de)

VERFAHREN ZUR AMPLIFIKATION VON NUKLEINSÄUREN

Title (fr)

PROCÉDÉ D'AMPLIFICATION D'ACIDES NUCLÉIQUES

Publication

EP 2454381 A1 (EN)

Application

EP 10734630 A

Priority

  • US 2010042323 W
  • EP 09165673 A

Abstract (en)

[origin: WO2011009073A1] The invention here concerns a method for increasing the uniformity of nucleic acid amplification. A method is described to add stopping periods during the ramp step(s), which minimizes the effect of temperature overshoot or undershoot. Many cyclers' algorithms cause the target temperature to be passed during ramp and then returned to within a short period of time. This overshoot or undershoot can cause non-uniformity between reactions in a micro¬ liter plate. The addition of stopping periods reduces the effects of overshoot or undershoot and increases uniformity between different reactions wells on a plate.

IPC 8 full level

C12Q 1/68 (2006.01)

CPC (source: EP)

C12Q 1/686 (2013.01)

C-Set (source: EP)

C12Q 1/686 + C12Q 2527/107 + C12Q 2527/101

 

Citation (search report)

See references of WO 2011009073A1

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

DOCDB simple family (publication)

WO 2011009073 A1 20110120; EP 2454381 A1 20120523

DOCDB simple family (application)

US 2010042323 W 20100716; EP 10734630 A 20100716