EP 2454381 A1 20120523 - A METHOD FOR AMPLIFICATION OF NUCLEIC ACIDS
Title (en)
A METHOD FOR AMPLIFICATION OF NUCLEIC ACIDS
Title (de)
VERFAHREN ZUR AMPLIFIKATION VON NUKLEINSÄUREN
Title (fr)
PROCÉDÉ D'AMPLIFICATION D'ACIDES NUCLÉIQUES
Publication
Application
Priority
- US 2010042323 W 20100716
- EP 09165673 A 20090716
Abstract (en)
[origin: WO2011009073A1] The invention here concerns a method for increasing the uniformity of nucleic acid amplification. A method is described to add stopping periods during the ramp step(s), which minimizes the effect of temperature overshoot or undershoot. Many cyclers' algorithms cause the target temperature to be passed during ramp and then returned to within a short period of time. This overshoot or undershoot can cause non-uniformity between reactions in a micro¬ liter plate. The addition of stopping periods reduces the effects of overshoot or undershoot and increases uniformity between different reactions wells on a plate.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/686 (2013.01)
Citation (search report)
See references of WO 2011009073A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
DOCDB simple family (publication)
DOCDB simple family (application)
US 2010042323 W 20100716; EP 10734630 A 20100716