EP 2457093 A1 20120530 - METHOD, PARTICULARLY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA), FOR IN VITRO DETECTION OF AMYLOID BETA AUTOANTIBODIES, MICROTITER PLATE, AND TEST KIT
Title (en)
METHOD, PARTICULARLY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA), FOR IN VITRO DETECTION OF AMYLOID BETA AUTOANTIBODIES, MICROTITER PLATE, AND TEST KIT
Title (de)
VERFAHREN, INSBESONDERE ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA), ZUM IN VITRO NACHWEIS VON AMYLOID BETA AUTOANTIKÖRPERN, MIKROTITERPLATTE UND TESTKIT
Title (fr)
PROCÉDÉ, NOTAMMENT UN DOSAGE PAR LA MÉTHODE ELISA, POUR DÉTECTER IN VITRO DES AUTO-ANTICORPS BÊTA AMYLOÏDE, PLAQUE DE MICROTITRATION ET KIT D'ESSAI
Publication
Application
Priority
- DE 102009034119 A 20090720
- DE 2010000817 W 20100714
Abstract (en)
[origin: WO2011009435A1] A method, particularly an enzyme-linked immunosorbent assay (ELISA), for the in-vitro detection of Aß autoantibodies in human serum and/or plasma contains the following steps: preparing an antigen-coated solid phase; incubating the solid phase with a blocking solution; incubating the solid phase with a sample to be examined; immunological detection of the Aß autoantibodies on the solid phase; and reading the detected results of the solid phase using a reading tool. According to the invention, the preparation of the antigen-coated solid phase advantageously includes incubating the solid phase with a coating solution in which the antigen is dissolved, said antigen having a peptide sequence selected from the group SEQ ID No. 1, SEQ ID No. 2 or SEQ ID No. 3.
IPC 8 full level
G01N 33/53 (2006.01); G01N 33/543 (2006.01)
CPC (source: EP US)
B01L 3/5085 (2013.01 - EP US); G01N 33/5306 (2013.01 - US); G01N 33/54386 (2013.01 - EP US); G01N 33/564 (2013.01 - EP US); G01N 33/6896 (2013.01 - EP US); B01L 2300/0829 (2013.01 - EP US); G01N 2800/2821 (2013.01 - EP US)
Citation (search report)
See references of WO 2011009435A1
Citation (examination)
- WO 2004056318 A2 20040708 - UNIV NEW YORK [US], et al
- DU Y ET AL: "REDUCED LEVELS OF AMYLOID BETA-PEPTIDE ANTIBODY IN ALZHEIMER DISEASE", NEUROLOGY, LIPPINCOTT WILLIAMS & WILKINS, PHILADELPHIA, US, vol. 57, no. 5, 11 September 2001 (2001-09-11), pages 801 - 805, XP009026218, ISSN: 0028-3878
- SOHN ET AL: "Reduced serum level of antibodies against amyloid beta peptide is associated with aging in Tg2576 mice", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 361, no. 3, 11 August 2007 (2007-08-11), pages 800 - 804, XP022196499, ISSN: 0006-291X, DOI: 10.1016/J.BBRC.2007.07.107
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
DOCDB simple family (publication)
DE 102009034119 A1 20110127; EP 2457093 A1 20120530; US 2013109033 A1 20130502; WO 2011009435 A1 20110127
DOCDB simple family (application)
DE 102009034119 A 20090720; DE 2010000817 W 20100714; EP 10747569 A 20100714; US 201013384726 A 20100714