EP 2459719 A1 20120606 - METHOD FOR PURIFYING ACTIVE GLA-DOMAIN COAGULATION PROTEINS
Title (en)
METHOD FOR PURIFYING ACTIVE GLA-DOMAIN COAGULATION PROTEINS
Title (de)
VERFAHREN ZUR REINIGUNG VON AKTIVEN GLA-DOMÄNE-KOAGULATIONSPROTEINEN
Title (fr)
PROCEDE POUR LA PURIFICATION DE PROTEINES DE LA COAGULATION A DOMAINE GLA ACTIVES
Publication
Application
Priority
- FR 0955413 A 20090731
- FR 2010051629 W 20100730
Abstract (en)
[origin: WO2011012831A1] The invention relates to a method for purifying biologically active GLA-domain coagulation proteins, comprising the following steps: a) bringing a sample that contains one or more GLA-domain coagulation proteins and may contain biologically inactive molecules of GLA-domain protein(s), into contact with an affinity substrate on which nucleic aptamers that bind specifically to at least one biologically active GLA-domain coagulation protein are immobilized, in order to form complexes between (i) said nucleic aptamers and (ii) said GLA-domain coagulation protein(s), b) releasing the GLA-domain coagulation protein(s) from the complexes formed in step a), and c) recovering said biologically active GLA-domain coagulation protein(s) in a purified form.
IPC 8 full level
C12N 15/115 (2010.01); C07K 14/745 (2006.01)
CPC (source: EP US)
C07K 14/745 (2013.01 - EP US); C12N 9/6432 (2013.01 - EP US); C12N 9/6437 (2013.01 - EP US); C12N 9/644 (2013.01 - EP US); C12N 15/115 (2013.01 - EP US); C12N 2310/16 (2013.01 - EP US); C12N 2310/351 (2013.01 - US); C12N 2330/31 (2013.01 - EP US); C12Y 304/21006 (2013.01 - EP US); C12Y 304/21021 (2013.01 - EP US); C12Y 304/21022 (2013.01 - EP US)
Citation (search report)
See references of WO 2011012831A1
Citation (third parties)
Third party :
WO 2010094901 A1 20100826 - LFB BIOTECHNOLOGIES [FR], et al
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
DOCDB simple family (publication)
WO 2011012831 A1 20110203; AU 2010277421 A1 20120315; AU 2010277421 B2 20151022; CA 2771850 A1 20110203; CA 2771850 C 20200218; CN 102666857 A 20120912; CN 102666857 B 20170308; EP 2459719 A1 20120606; FR 2948665 A1 20110204; FR 2948665 B1 20110923; IL 218261 A0 20120430; KR 20120105419 A 20120925; US 2012271039 A1 20121025; US 2016138001 A1 20160519; US 9212365 B2 20151215; US 9850477 B2 20171226
DOCDB simple family (application)
FR 2010051629 W 20100730; AU 2010277421 A 20100730; CA 2771850 A 20100730; CN 201080043798 A 20100730; EP 10762958 A 20100730; FR 0955413 A 20090731; IL 21826112 A 20120222; KR 20127005553 A 20100730; US 201013392989 A 20100730; US 201514936896 A 20151110