Global Patent Index - EP 2471549 A1

EP 2471549 A1 20120704 - Adhesin as immunogen against Escherichia coli

Title (en)

Adhesin as immunogen against Escherichia coli

Title (de)

Adhäsin als Immunogen gegen Escherichia coli

Title (fr)

Adhésine en tant qu'immunogène contre l'Escherichia coli

Publication

EP 2471549 A1 20120704 (EN)

Application

EP 11006574 A 20060110

Previously filed application

PCT/US2006/000660 20060110 WO

Priority

  • EP 06717818 A 20060110
  • US 64277105 P 20050111
  • US 2006000660 W 20060110

Abstract (en)

An immunogenic composition wherein said composition is a complex comprising an amino acid sequence encoding a whole or an antigenic polypetide fragment of Escherichia coli fimbrial adhesin linked at the C-terminal end of said fimbrial adhesin to a linker which is operatively linked at the C-terminus of said linker to a whole or an antigenic polypeptide fragment of Escherichia coli major structural fimbrial subunit.

IPC 8 full level

A01N 63/00 (2006.01); A61K 39/02 (2006.01); A61K 39/08 (2006.01); A61K 39/40 (2006.01); A61K 45/00 (2006.01); C07K 14/245 (2006.01); C07K 16/12 (2006.01); A61K 39/00 (2006.01)

CPC (source: EP US)

A61K 39/0258 (2013.01 - EP US); A61P 1/12 (2017.12 - EP); A61P 31/04 (2017.12 - EP); A61P 43/00 (2017.12 - EP); C07K 14/245 (2013.01 - EP US); C07K 16/1232 (2013.01 - EP US); A61K 38/00 (2013.01 - EP US); A61K 2039/523 (2013.01 - EP US); A61K 2039/545 (2013.01 - EP US); C07K 2317/55 (2013.01 - EP US); C07K 2319/00 (2013.01 - US); C07K 2319/35 (2013.01 - EP US); Y02A 50/30 (2017.12 - EP US)

Citation (applicant)

  • BLACK, R. E.: "Epidemiology of travelers' diarrhea and relative importance of various pathogens", REV INFECT DIS, vol. 12, no. 1, 1990, pages S73 - S79
  • HUILAN, S.; L. G. ZHEN; M. M. MATHAN; M. M. MATHEW; J. OLARTE; R. ESPEJO; U. KHIN MAUNG; M. A.GHAFOOR; M. A. KHAN; Z. SAMI ET AL.: "Etiology of acute diarrhoea among children in developing countries: a multicentre study in five countries", BULL WORLD HEALTH ORGAN, vol. 69, 1991, pages 549 - 55
  • NATARO, J. P.; J. B. KAPER: "Diarrheagenic Escherichia coli", CLIN MICROBIOL REV, vol. 11, 1998, pages 142 - 201
  • GAASTRA, W.; A. M. SVENNERHOLM: "Colonization factors of human enterotoxigenic Escherichia coli (ETEC", TRENDS MICROBIOL, vol. 4, 1996, pages 444 - 452
  • EVANS, D. G.; R. P. SILVER; D. J. EVANS, JR.; D. G. CHASE; S. L. GORBACH.: "Plasmid-controlled colonization factor associated with virulence in Esherichia coli enterotoxigenic for humans", INFECT IMMUN, vol. 12, 1975, pages 656 - 667
  • GREWAL, H. M.; H. VALVATNE; M. K. BHAN; L. VAN DIJK; W. GAASTRA; H. SOMMERFELT: "A new putative fimbrial colonization factor, CS19, of human enterotoxigenic Escherichia coli", INFECT IMMUN, vol. 65, 1997, pages 507 - 513
  • KHALIL, S. B.; F. J. CASSELS; H. I. SHAHEEN; L. K. PANNELL; K. A. KAMAL; B. T. PITTNER; M. MANSOUR; R. FRENCK; S. J. SAVARINO; P., 100TH GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, 2000
  • FROEHLICH, B. J.; A. KARAKASHIAN; L. R. MELSEN; J. C. WAKEFIELD; J. R. SCOTT: "CooC and CooD are required for assembly of CS pili.", MOL MICROBIOL, vol. 12, 1994, pages 387 - 401
  • FROEHLICH, B. J.; A. KARAKASHIAN; H. SAKELLARIS; J. R. SCOTT.: "Genes for CS2 pili of enterotoxigenic Escherichia coli and their interchangeability with those for CS pili", INFECT IMMUN, vol. 63, 1995, pages 4849 - 56
  • JORDI, B. J. A. M.; G. A. WILLSHAW; A. M. VAN DER ZEIJST; W. GAASTRA: "The complete nucleotide sequence of region 1 of the CFA/1 funbrial operon of human enterotoxigenic Escherichia coli", DNA SEQ, vol. 2, 1992, pages 257 - 263
  • PEREZ-CASAL, J.; J. S. SWARTLEY; J. R. SCOTT.: "Gene encoding the major subunit of CS pili of human enterotoxigenic Escherichia coli", INFECT IMMUN, vol. 58, 1990, pages 3594 - 3600
  • SCOTT, J. R.; J. C. WAKEFIELD; P. W. RUSSELL; P. E. ORNDORFF; B. J. FROEHLICH: "CooB is required for assembly but not transport of CS 1 pilin", MOL MICROBIOL, vol. 6, 1992, pages 293 - 300
  • GAASTRA, W.; H. SOMMERFELT; L. VAN DIJK; J. G. KUSTERS; A. M. SVENNERHOLM; H. M. GREWAL: "Antigenic variation within the subunit protein of members of the colonization factor antigen I group of fimbrial proteins in human enterotoxigenic Escherichia coli", INT J MED MICROBIOL, vol. 292, 2002, pages 43 - 50
  • RAMER, S. W.; G. K. SCHOOLNIK; C. Y. WU; J. HWANG; S. A. SCHMIDT; D. BIEBER: "The Type IV pilus assembly complex: Biogenic interactions among the bundle forming pilus proteins of enteropathogenic Escherichia coli", J BACTERIOL, vol. 184, 2002, pages 3457 - 65
  • SOTO, G. E.; S. J. HULTGREN: "Bacterial adhesins: common themes and variations in architecture and assembly", J BACTERIOL, vol. 181, 1999, pages 1059 - 1071
  • LOW, D.; B. BRAATEN; M. VAN DER WOUDE: "Escherichia coli and Salmonella: Cellular and Molecular Biology", vol. 1, 1996, ASM PRESS, article "Fimbriae", pages: 146 - 157
  • SAKELLARIS, H.; J. R. SCOTT.: "New tools in an old trade: CS1 pilus morphogenesis", MOL MICROBIOL, vol. 30, 1998, pages 681 - 7
  • SAKELLARIS, H.; V. R. PENUMALLI; J. R. SCOTT.: "The level of expression of the minor pilin subunit, CooD, determines the number of CS1 pili assembled on the cell surface of Escherichia coli", J BACTERIOL, vol. 181, 1999, pages 1694 - 7
  • BUHLER, T.; H. HOSCHUTZKY; K. JANN: "Analysis of colonization factor antigen I, an adhesin of enterotoxigenic Escherichia coli 078:H11: fimbrial morphology and location of the receptor-binding site", INFECT IMMUN, vol. 59, 1991, pages 3876 - 3882
  • SAKELLARIS, H.; G. P. MUNSON; J. R. SCOTT.: "A conserved residue in the tip proteins of CS1 and CFA/I pili of enterotoxigenic Escherichia coli that is essential for adherence", PROC NATL ACAD SCI, vol. 96, 1999, pages 12828 - 12832
  • ANANTHA, RAVI P.; A. L. MCVEIGH; L.H. LEE; M. K. AGNEW; F.J. CASSELS; D. A. SCOTT; T S. WHITTAM; S. J. SAVARINO: "Evolutionary and functional relationships of colonization factor antigen I and other class 5 adhesive fimbriae of enterotoxigenic Escherichia coli", INF AND IMM., vol. 72, 2004, pages 7190 - 7201
  • VIBOUD, G. I.; M. M. MCCONNELL; A. HELANDER; A. M. SVENNERHOLM: "Binding of enterotoxigenic Escherichia coli expressing different colonization factors to tissue-cultured Caco-2 cells and to isolated human enterocytes", MICROB PATHOGEN, vol. 21, 1996, pages 139 - 147
  • KUEHN MJ; J. HEUSER; S. NORMARK; S.J. HULTGREN: "P pili in uropathogenic E. coli are composite fibres with distinct fibrillar adhesive tips", NATURE, vol. 356, 1992, pages 252 - 5
  • SAUER FG; K. FUTTERER; J.S. PINKNER; K.W. DODSON; S.J. HULTGREN; G. WAKSMAN: "Structural basis of chaperone function and pilus biogenesis", SCIENCE, vol. 285, 1999, pages 1058 - 61
  • CHOUDHURY D; A. THOMPSON; V. STOJANOFF ET AL.: "X-ray structure of the FimC-FimH chaperone-adhesin complex from uropathogenic Escherichia coli", SCIENCE, vol. 285, 1999, pages 1061 - 6
  • BARNHART MM; PINKNER JS; SOTO GE ET AL.: "From the cover: PapD-like chaperones provide the missing information for folding of pilin proteins", PROC. NATL. ACAD. SCI. U.S.A., vol. 97, 2000, pages 7709 - 14
  • KRASAN GP; SAUER FG; CUTTER D ET AL.: "Evidence for donor strand complementation in the biogenesis of Haemophilus influenzae haemagglutinating pili", MOL. MICROBIOL., vol. 35, 2000, pages 1335 - 47
  • ZAVIALOV AV; KERSLEY J; KORPELA T; ZAV'YALOV VP; MACINTYRE S; KNIGHT SD: "Donor strand complementation mechanism in the biogenesis of non-pilus systems", MOL. MICROBIOL., vol. 45, 2002, pages 983 - 995
  • RAO, M. R.; R. ABU-ELYAZEED; S. J. SAVARINO; A. B. NAFICY; T. F. WIERZBA; I. ABDEL MESSIH; H. SHAHEEN; R. W. FRENCK; A. M. SVENNER: "High disease burden due to enterotoxigenic Escherichia coli diarrhea in early life among rural Egyptian children", J CLIN MICROBIOL, vol. 41, 2003, pages 4862 - 4864
  • CRAVIOTO, A.; S. M. SCOTLAND; B. ROWE: "Hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non enterotoxigenic Escherichia coli isolated from humans", INFECT IMMUN, vol. 36, 1982, pages 189 - 197
  • SAMBROOK, J.; E. F. FRITSCH; T. MANIATIS: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
  • EVANS, D..G.; D. J. EVANS, JR.; W. S. TJOA; H. L. DUPONT: "Detection and characterization of colonization factor of enterotoxigenic Escherichia coli isolated from adults with diarrhea", INFECT IMMUN, vol. 19, 1978, pages 727 - 736
  • DARFEUILLE-MICHAUD, A.; D. AUBEL; G. CHAUVIERE; C. RICH; M. BOURGES; A. SERVIN; B. JOLY: "Adhesion of enterotoxigenic Escherichia coli to the human colon carcinoma cell line Caco-2 in culture", INFECT IMMUN, vol. 58, 1990, pages 893 - 902
  • KHALIL, S. B.; F. J. CASSELS; H. 1. SHAHEEN; L. K. PANNELL; N. EL-GHORAB; K. KAMAL; M. MANSOUR; S. J. SAVARINO; L. F. PERUSKI, JR.: "Characterization of an enterotoxigenic Escherichia coli strain from Africa expressing a putative colonization factor", INFECT IMMUN, vol. 67, 1999, pages 4019 - 4026
  • HALL, R. H.; D. R. MANEVAL, JR.; J. H. COLLINS; J. L. THEIBERT; M. M. LEVINE: "Purification and analysis of colonization factor antigen I, coli surface antigen 1, and coli surface antigen 3 fimbriae from enterotoxigenic Escherichia coli.", J BACTERIOL, vol. 171, 1989, pages 6372 - 6374
  • HESS, S.; F. J. CASSELS; L. K. PANNELL: "Identification and characterization of hydrophobic Escherichia coli virulence proteins by liquid chromatography- electrospray ionization mass spectrometry", ANAL BIOCHEM, vol. 302, 2002, pages 123 - 130
  • SAKELLARIS H; D.P. BALDING; J.R. SCOTT.: "Assembly proteins of CS pili of enterotoxigenic Escherichia coli", MOL. MICROBIOL., vol. 21, 1996, pages 529 - 41
  • BENDTSEN J.D.; H. NIELSEN H; G. VON HEIJNE; S. BRUNAK.: "Improved prediction of signal peptides: SignalP 3.0", J MOL BIOL, vol. 340, 2004, pages 783 - 95
  • HALL, R.H.; D.R. MANEVAL, JR.; J.H. COLLINS; J.L. THEIBERT; M.M. LEVINE: "Purification and analysis of colonization factor antigen I, coli surface antigen 1, and coli surface antigen 3 fimbriae from enterotoxigenic Escherichia coli", J. BACT., vol. 171, 1989, pages 6372 - 6374
  • JALAJAKUMARI, M.B.; C.J. THOMAS; R. HALTER; P.A. MANNING: "Genes for biosysnthesis and assembly of CS3 pili of CFA/II enterotoigenic Escherichia coli: novel regulation of pilus production by bypassing an amber codon", MOL. MICRO, vol. 3, 1989, pages 1685 - 1695
  • PERLER, F.B.: "InBase, the Intein Database", NUC. ACIDS. RES., vol. 30, 2002, pages 383 - 384
  • HUNG, D.L.; S.D. KNIGHT; R.M. WOODS; J.S. PINKNER; S.J. HULTGREN: "Molecular basis of two subfamilies of immunoglobulin-like chaperones", EMBO J., vol. 15, 1996, pages 3792 - 3805
  • SOTO, G.E.; S.J. HULTGREN.: "Bacterial adhesins: common themes and variations in architecture and assembly", J. BACT., vol. 181, 1999, pages 1059 - 1071
  • ZAVIALOV, A.V.; J. BERGLUND; A.F. PUDNEY ET AL.: "Structure and biogenesis of the capsular Fl antigen from Yersinia pestis: preserved folding energy drives fiber formation", CELL, vol. 113, 2003, pages 587 - 596

Citation (search report)

  • [XA] WO 02059156 A2 20020801 - MEDIMMUNE INC [US], et al
  • [XA] WO 0104148 A2 20010118 - MEDIMMUNE INC [US]
  • [A] WO 02064162 A2 20020822 - US GOV SEC ARMY [US], et al
  • [A] BYRD W ET AL: "Mucosal immunization of BALB/c mice using enterotoxigenic Escherichia coli colonization factors CFA/I and CS6 administered with and without a mutant heat-labile enterotoxin", VACCINE, BUTTERWORTH SCIENTIFIC. GUILDFORD, GB, vol. 21, no. 17-18, 16 May 2003 (2003-05-16), pages 1884 - 1893, XP004421100, ISSN: 0264-410X
  • [A] ANDERSON KIRSTINE L ET AL: "An atomic resolution model for assembly, architecture, and function of the Dr adhesins", MOLECULAR CELL, vol. 15, no. 4, 27 August 2004 (2004-08-27), pages 647 - 657, XP002521103, ISSN: 1097-2765
  • [A] SMYTH C J ET AL: "Fimbrial adhesins: similarities and variations in structure and biogenesis.", FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY 1 DEC 1996, vol. 16, no. 2, 1 December 1996 (1996-12-01), pages 127 - 139, XP002521102, ISSN: 0928-8244
  • [AP] POOLE STEVEN T ET AL: "Donor strand complementation governs intersubunit interaction of fimbriae of the alternate chaperone pathway.", MOLECULAR MICROBIOLOGY MAR 2007, vol. 63, no. 5, March 2007 (2007-03-01), pages 1372 - 1384, XP002521121, ISSN: 0950-382X
  • [A] SAUER FREDERIC G ET AL: "Structural basis of chaperone function and pilus biogenesis", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, WASHINGTON, DC; US, vol. 285, no. 5430, 13 August 1999 (1999-08-13), pages 1058 - 1061, XP002156831, ISSN: 0036-8075, DOI: 10.1126/SCIENCE.285.5430.1058
  • [A] ANANTHA RAVI P ET AL: "Evolutionary and functional relationships of colonization factor antigen i and other class 5 adhesive fimbriae of enterotoxigenic Escherichia coli", INFECTION AND IMMUNITY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 72, no. 12, 1 December 2004 (2004-12-01), pages 7190 - 7201, XP002560521, ISSN: 0019-9567, DOI: 10.1128/IAI.72.12.7190-7201.2004

Designated contracting state (EPC)

AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI SK TR

DOCDB simple family (publication)

US 2006153878 A1 20060713; US 9079945 B2 20150714; AU 2006205128 A1 20060720; AU 2006205128 B2 20130328; CA 2592444 A1 20060720; CA 2592444 C 20150407; EP 1855718 A2 20071121; EP 1855718 A4 20090513; EP 1855718 B1 20161012; EP 2471549 A1 20120704; ES 2610395 T3 20170427; JP 2008526967 A 20080724; JP 2013253083 A 20131219; JP 2016053068 A 20160414; JP 5554471 B2 20140723; JP 6147120 B2 20170614; US 10017547 B2 20180710; US 10501504 B2 20191210; US 2015266932 A1 20150924; US 2019055292 A1 20190221; WO 2006076285 A2 20060720; WO 2006076285 A3 20081023

DOCDB simple family (application)

US 34000306 A 20060110; AU 2006205128 A 20060110; CA 2592444 A 20060110; EP 06717818 A 20060110; EP 11006574 A 20060110; ES 06717818 T 20060110; JP 2007551308 A 20060110; JP 2013142013 A 20130705; JP 2015219633 A 20151109; US 2006000660 W 20060110; US 201514733421 A 20150608; US 201815991443 A 20180529