EP 2473255 A4 20161102 - METHODS FOR ACQUISITION AND DEDUCTIVE ANALYSIS OF MIXED FRAGMENT PEPTIDE MASS SPECTRA
Title (en)
METHODS FOR ACQUISITION AND DEDUCTIVE ANALYSIS OF MIXED FRAGMENT PEPTIDE MASS SPECTRA
Title (de)
VERFAHREN ZUR ERFASSUNG UND DEDUKTIVEN ANALYSE VON MISCHFRAGMENTPEPTID-MASSENSPEKTREN
Title (fr)
PROCÉDÉS D'ACQUISITION ET D'ANALYSE DÉDUCTIVE DE SPECTRES DE MASSE DE PEPTIDES À FRAGMENTS MIXTES
Publication
Application
Priority
- US 55065909 A 20090831
- US 2010046669 W 20100825
Abstract (en)
[origin: US2011049351A1] A method for acquiring and analyzing polypeptide mass spectra comprising: creating first and second sets of fragmented ions using first and second fragmentation techniques; obtaining mass spectra of the first and second sets of fragmented ions; searching a database of fragments of a first fragment pair type and of a second fragment pair type to respectively provide a set of M ranked and a set of N ranked best matching polypeptide sequences; subtracting synthetic spectra corresponding to each of the M and N ranked sequences from the mass spectra to provide first and second sets of modified mass spectra; searching the database of fragments of the second fragment pair type and of the first fragment pair type to provide third and fourth sets of polypeptide sequences providing best matches to the first and second sets of modified spectra, respectively; and creating a cumulative ranking of the third and fourth sets.
IPC 8 full level
G16B 20/00 (2019.01)
CPC (source: EP US)
G01N 33/6848 (2013.01 - EP US); G16B 20/00 (2019.01 - EP US); H01J 49/0027 (2013.01 - EP US); H01J 49/0045 (2013.01 - EP US)
Citation (search report)
- [I] EP 1457776 A2 20040915 - AGILENT TECHNOLOGIES INC [US]
- [I] DOMON B. ET AL: "Electron Transfer Dissociation in Conjunction with Collision Activation To Investigate the Drosophila melanogaster Phosphoproteome", JOURNAL OF PROTEOME RESEARCH., vol. 8, no. 6, 12 May 2009 (2009-05-12), US, pages 2633 - 2639, XP055300797, ISSN: 1535-3893, DOI: 10.1021/pr800834e
- [I] BAILEY CH. M. ET AL: "SLoMo: Automated Site Localization of Modifications from ETD/ECD Mass Spectra", JOURNAL OF PROTEOME RESEARCH., vol. 8, no. 4, 10 March 2009 (2009-03-10), US, pages 1965 - 1971, XP055300796, ISSN: 1535-3893, DOI: 10.1021/pr800917p
- See references of WO 2011025833A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
DOCDB simple family (publication)
US 2011049351 A1 20110303; US 8153961 B2 20120410; EP 2473255 A1 20120711; EP 2473255 A4 20161102; WO 2011025833 A1 20110303
DOCDB simple family (application)
US 55065909 A 20090831; EP 10812588 A 20100825; US 2010046669 W 20100825