EP 2484671 A4 20130724 - FLUORESCENT MOLECULE AND METHOD FOR DETECTING TARGET NUCLEIC ACID
Title (en)
FLUORESCENT MOLECULE AND METHOD FOR DETECTING TARGET NUCLEIC ACID
Title (de)
FLUORESZIERENDES MOLEKÜL UND VERFAHREN ZUM NACHWEIS EINER ZIELNUKLEINSÄURE
Title (fr)
MOLÉCULE FLUORESCENTE ET PROCÉDÉ DE DÉTECTION D'ACIDE NUCLÉIQUE CIBLE
Publication
Application
Priority
- JP 2009229866 A 20091001
- JP 2010067287 W 20101001
Abstract (en)
[origin: EP2484671A1] Provided are a fluorescent on/off switchable compound for a gene analysis, which is highly stable and highly sensitive, and enables amplification and observation of a trace gene signal, and a labeling reagent for detection of a bio-related material, which uses the fluorescent on/off switchable compound. A compound represented by the following formula (I'): wherein X is a hydrogen atom or a carboxylic acid-protecting group, Y is a 2,4-dinitrobenzenesulfonyl group, a 2-cyano-4-nitrobenzenesulfonyl group or a 5-nitropyridin-2-ylsulfonyl group, R 1 , R 2 , R 3 and R 4 are each independently a hydrogen atom, a halogen atom, a hydroxyl group, an alkyl group having a carbon number of 1 to 6, an alkoxy group having a carbon number of 1 to 6, an aryl group having a carbon number of 6 to 10, or a cyano group.
IPC 8 full level
C07D 311/16 (2006.01); C07D 405/12 (2006.01); C12N 15/09 (2006.01); C12Q 1/02 (2006.01); C12Q 1/68 (2006.01); G01N 21/78 (2006.01)
CPC (source: EP US)
C07D 311/16 (2013.01 - EP US); C07D 405/12 (2013.01 - EP US); C07H 21/02 (2013.01 - EP US); C12Q 1/6816 (2013.01 - EP US); C12Q 1/6818 (2013.01 - EP US); G01N 33/533 (2013.01 - EP US); G01N 33/582 (2013.01 - EP US); Y02P 20/55 (2015.11 - EP US); Y10T 436/143333 (2015.01 - EP US)
C-Set (source: EP US)
Citation (search report)
- [YA] US 2006105412 A1 20060518 - MAEDA HATSUO [JP], et al
- [YA] SHIBATA ET AL: "Rhodamine-based fluorogenic probe for imaging biological thiol", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, PERGAMON, ELSEVIER SCIENCE, GB, vol. 18, no. 7, 10 March 2008 (2008-03-10), pages 2246 - 2249, XP022574939, ISSN: 0960-894X, DOI: 10.1016/J.BMCL.2008.03.014
- See references of WO 2011040612A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
EP 2484671 A1 20120808; EP 2484671 A4 20130724; JP 5733760 B2 20150610; JP WO2011040612 A1 20130228; US 2012196377 A1 20120802; US 8709721 B2 20140429; WO 2011040612 A1 20110407
DOCDB simple family (application)
EP 10820726 A 20101001; JP 2010067287 W 20101001; JP 2011534352 A 20101001; US 201013499826 A 20101001