Global Patent Index - EP 2488010 A4

EP 2488010 A4 20140129 - MANIPULATION OF FLAVONOID BIOSYNTHETIC PATHWAY

Title (en)

MANIPULATION OF FLAVONOID BIOSYNTHETIC PATHWAY

Title (de)

MANIPULATION DES BIOSYNTHESE-SIGNALWEGES VON FLAVONOIDEN

Title (fr)

MANIPULATION D'UNE VOIE DE BIOSYNTHÈSE DE FLAVONOÏDES

Publication

EP 2488010 A4 20140129 (EN)

Application

EP 10822907 A 20101015

Priority

  • AU 2009905057 A 20091016
  • AU 2009905058 A 20091016
  • AU 2009905063 A 20091016
  • AU 2010001362 W 20101015

Abstract (en)

[origin: WO2011044632A1] The present invention relates to a method of identifying a gene encoding a polypeptide or polypeptide isoform which is substantially more active in either a proanthocyanidin (PA) or anthocyanin (ANT) pathway of a plant, said method including providing material from said plant; and an oligonucleotide probe capable of hybridizing with RNA from a gene encoding a polypeptide which is active in a flavonoid biosynthetic pathway; extracting RNA from said plant material; hybridizing the oligonucleotide probe with the RNA to generate an expression profile; measuring PA and/or ANT levels in said plant material to generate a metabolic profile; comparing said expression profile with said metabolite profile to identify said gene encoding a polypeptide or polypeptide isoform which is substantially active in either a PA or ANT pathway.

IPC 8 full level

A01H 5/00 (2006.01); C12N 15/29 (2006.01); C12N 15/82 (2006.01); C12Q 1/68 (2006.01)

CPC (source: EP US)

C12N 15/8243 (2013.01 - EP US); C12N 15/825 (2013.01 - EP US); C12Q 1/6895 (2013.01 - EP US); C12Q 2600/158 (2013.01 - EP US)

Citation (search report)

  • [X] WO 2008134372 A2 20081106 - SAMUEL ROBERTS NOBLE FOUND INC [US], et al
  • [X] WO 2009003216 A1 20090108 - AGRICULTURE VICTORIA SERV PTY [AU], et al
  • [A] AIDYN MOURADOV ET AL: "Molecular dissection of proanthocyanidin and anthocyanidin biosynthesis in white clover (trifolium repens)", 1 January 2009, MOLECULAR BREEDING OF FORAGE AND TURF, SPRINGER, PAGE(S) 133 - 139, ISBN: 978-0-387-79143-2, XP009174672
  • [A] XIE D-Y ET AL: "Role of anthocyanidin reductase, encoded by BANYULS in plant flavonoid biosynthesis", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, WASHINGTON, DC; US, vol. 299, 17 January 2003 (2003-01-17), pages 396 - 399, XP002982939, ISSN: 0036-8075, DOI: 10.1126/SCIENCE.1078540
  • [XP] ABEYNAYKE, SHAMILA WEERAKOON: "Analysis of genes involved in condensed tannin biosynthesis in white clover (trifolium repens. L).", 2009, XP002717356, Retrieved from the Internet <URL:http://library.latrobe.edu.au/record=b2543251> [retrieved on 20131127]
  • [X] JAAKOLA LAURA ET AL: "Expression of genes involved in anthocyanin biosynthesis in relation to anthocyanin, proanthocyanidin, and flavonol levels during bilberry fruit development", PLANT PHYSIOLOGY (ROCKVILLE), vol. 130, no. 2, October 2002 (2002-10-01), pages 729 - 739, XP002717357, ISSN: 0032-0889
  • [T] S. W. ABEYNAYAKE ET AL: "Biosynthesis of Proanthocyanidins in White Clover Flowers: Cross Talk within the Flavonoid Pathway", PLANT PHYSIOLOGY, vol. 158, no. 2, December 2011 (2011-12-01), pages 666 - 678, XP055090636, ISSN: 0032-0889, DOI: 10.1104/pp.111.189258
  • See references of WO 2011044632A1

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

DOCDB simple family (publication)

WO 2011044632 A1 20110421; AU 2010306410 A1 20120503; AU 2010306410 B2 20150813; CA 2777249 A1 20110421; CA 2777249 C 20210309; DK 2488010 T3 20180305; EP 2488010 A1 20120822; EP 2488010 A4 20140129; EP 2488010 B1 20171206; NZ 599243 A 20140829; US 2012204289 A1 20120809

DOCDB simple family (application)

AU 2010001362 W 20101015; AU 2010306410 A 20101015; CA 2777249 A 20101015; DK 10822907 T 20101015; EP 10822907 A 20101015; NZ 59924310 A 20101015; US 201013501918 A 20101015