EP 2526199 A2 20121128 - METHODS OF GENERATING ZINC FINGER NUCLEASES HAVING ALTERED ACTIVITY
Title (en)
METHODS OF GENERATING ZINC FINGER NUCLEASES HAVING ALTERED ACTIVITY
Title (de)
VERFAHREN ZUR ERZEUGUNG VON ZINKFINGERNUKLEASEN MIT VERÄNDERTER WIRKUNG
Title (fr)
PROCÉDÉS DE PRODUCTION DE NUCLÉASES EN DOIGT DE ZINC AYANT UNE ACTIVITÉ MODIFIÉE
Publication
Application
Priority
- US 32903510 P 20100428
- US 29759810 P 20100122
- US 2011022154 W 20110121
Abstract (en)
[origin: WO2011091324A2] Provided herein are zinc linger nucleases having altered, arid in particular, improved catalytic activity and methods of generating such nucleases. Accordingly, there are provided methods for identifying improved catalytic activity of a ZFN by expressing a mutated zinc finger nuclease in a cell containing a reporter construct with a toxic gene, and a zinc finger nuclease cleavage site that is recognized by the ZFN. Survival of the cell is positively correlated with catalytic activity of the ZFN; thus, libraries of mutated ZFKs may be selected for altered catalytic activity based on relative survival rates, Methods of using identified ZFNs are also provided.
IPC 8 full level
C12Q 1/34 (2006.01); C12N 9/14 (2006.01); C12N 15/55 (2006.01); C07K 14/47 (2006.01); C12N 15/10 (2006.01)
CPC (source: EP)
C07K 14/4703 (2013.01); C12N 9/22 (2013.01); C12N 15/1034 (2013.01); C12N 15/1086 (2013.01); G01N 2333/922 (2013.01)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2011091324 A2 20110728; WO 2011091324 A3 20111222; WO 2011091324 A8 20120830; EP 2526199 A2 20121128; EP 2526199 A4 20130807
DOCDB simple family (application)
US 2011022154 W 20110121; EP 11735277 A 20110121