EP 2546628 A1 20130116 - Filter support with a phase-changing medium
Title (en)
Filter support with a phase-changing medium
Title (de)
Filterhalter für phasenwechselndes Medium
Title (fr)
Support de filtre avec support de changement de phase
Publication
Application
Priority
US 201161507315 P 20110713
Abstract (en)
The present invention relates to a method for processing isolated cells, in particular rare cells such as circulating tumor cells, to render them suitable for optical imaging, comprising the steps of (a) depositing filter material comprising captured cells on a phase-change medium; (b) melting said phase-change medium until the medium is spread out below said filter material; and (c) lowering the temperature of said phase-change medium until the medium solidifies, resulting in an optically flat filter comprising said cells at a fixed position. The method may additionally comprise an initial step of capturing a cell on a filter material. The phase-change medium, preferably paraffin wax, may comprise a porous, or mesh-like structure allowing the passage of a fluid through the medium. The phase-change medium may further be mounted on a carrier such as a glass carrier or a polymer material carrier. The present invention further relates to processed filter material comprising isolated cells suitable for optical imaging, which is obtainable by the method; the use of such filter material for diagnostic, histological, microbiological, biochemical, oncologic, or hematologic analysis, as well as a device for isolating cells or processing cells to render them suitable for optical imaging.
IPC 8 full level
G01N 1/36 (2006.01); G01N 33/48 (2006.01); G02B 21/34 (2006.01)
CPC (source: EP US)
G01N 1/2813 (2013.01 - EP US); G01N 1/30 (2013.01 - US); G01N 1/36 (2013.01 - EP US); G01N 33/48 (2013.01 - EP US); G01N 33/569 (2013.01 - EP US); G02B 21/34 (2013.01 - EP US)
Citation (applicant)
- WEINBERG: "The biology of cancer", 2007, GARLAND SCIENCE
- ZHENG ET AL., BIOMED MICRODEVICES, vol. 13, 2011, pages 203 - 213
- SAGE, NEECE, J CLIN MICROBIOL., vol. 20, no. 1, 1984, pages 5 - 8
Citation (search report)
- [Y] DE 4117833 A1 19921203 - MEDITE GES FUER MEDIZINTECHNIK [DE]
- [XY] DE 2928790 A1 19810212 - SARTORIUS GMBH
- [XY] WO 2004041994 A1 20040521 - UNIV MASSACHUSETTS [US]
- [YA] WO 9005320 A1 19900517 - SHIBASAKI MICHIRO [JP]
- [X] DE 3319678 A1 19841206 - HERAEUS CHRIST GMBH [DE]
- [Y] EP 0122581 A2 19841024 - TERUMO CORP [JP]
- [XA] DE 3023849 A1 19820121 - SARTORIUS GMBH [DE]
- [A] US 5547571 A 19960820 - TSUBAKINO MOTOHIRO [JP]
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
DOCDB simple family (publication)
EP 2546628 A1 20130116; CN 103649714 A 20140319; CN 103649714 B 20170623; EP 2732261 A1 20140521; JP 2014523534 A 20140911; JP 6096769 B2 20170315; MX 2014000303 A 20140220; MX 344505 B 20161219; US 2014147883 A1 20140529; US 9291533 B2 20160322; WO 2013008142 A1 20130117
DOCDB simple family (application)
EP 11174313 A 20110718; CN 201280034576 A 20120704; EP 12745568 A 20120704; IB 2012053408 W 20120704; JP 2014519660 A 20120704; MX 2014000303 A 20120704; US 201214131757 A 20120704