EP 2635708 A1 20130911 - ANALYSIS OF FRAGMENTED GENOMIC DNA IN DROPLETS
Title (en)
ANALYSIS OF FRAGMENTED GENOMIC DNA IN DROPLETS
Title (de)
ANALYSE FRAGMENTIERTER GENOMISCHER DNA IN TRÖPFCHEN
Title (fr)
ANALYSE D'ADN GÉNOMIQUE FRAGMENTÉ DANS DES GOUTTELETTES
Publication
Application
Priority
- US 97682710 A 20101222
- US 40910610 P 20101101
- US 2011058854 W 20111101
Abstract (en)
[origin: WO2012061442A1] Method of analyzing genomic DNA. Genomic DNA including a target may be obtained. The genomic DNA may be fragmented volitionally to produce fragmented DNA. The fragmented DNA may be passed through a droplet generator to generate aqueous droplets containing the fragmented DNA. An assay may be performed on the droplets to determine a level of the target. In some embodiments, the droplets may contain the genomic DNA at a concentration of at least about five nanograms per microliter, the droplets may be generated at a droplet generation frequency of at least about 50 droplets per second, the droplets may have an average volume of less than about 10 nanoliters per droplet, the droplets may generated at a flow rate of greater than about 50 nanoliters per second, or any combination thereof.
IPC 8 full level
C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/686 (2013.01)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2012061442 A1 20120510; AU 2011323507 A1 20130613; CA 2816702 A1 20120510; CN 103443289 A 20131211; EP 2635708 A1 20130911; EP 2635708 A4 20141029; JP 2013541347 A 20131114; SG 190076 A1 20130628
DOCDB simple family (application)
US 2011058854 W 20111101; AU 2011323507 A 20111101; CA 2816702 A 20111101; CN 201180052831 A 20111101; EP 11838712 A 20111101; JP 2013537769 A 20111101; SG 2013033238 A 20111101