Global Patent Index - EP 2635708 A1

EP 2635708 A1 2013-09-11 - ANALYSIS OF FRAGMENTED GENOMIC DNA IN DROPLETS

Title (en)

ANALYSIS OF FRAGMENTED GENOMIC DNA IN DROPLETS

Title (de)

ANALYSE FRAGMENTIERTER GENOMISCHER DNA IN TRÖPFCHEN

Title (fr)

ANALYSE D'ADN GÉNOMIQUE FRAGMENTÉ DANS DES GOUTTELETTES

Publication

EP 2635708 A1 (EN)

Application

EP 11838712 A

Priority

  • US 97682710 A
  • US 40910610 P
  • US 2011058854 W

Abstract (en)

[origin: WO2012061442A1] Method of analyzing genomic DNA. Genomic DNA including a target may be obtained. The genomic DNA may be fragmented volitionally to produce fragmented DNA. The fragmented DNA may be passed through a droplet generator to generate aqueous droplets containing the fragmented DNA. An assay may be performed on the droplets to determine a level of the target. In some embodiments, the droplets may contain the genomic DNA at a concentration of at least about five nanograms per microliter, the droplets may be generated at a droplet generation frequency of at least about 50 droplets per second, the droplets may have an average volume of less than about 10 nanoliters per droplet, the droplets may generated at a flow rate of greater than about 50 nanoliters per second, or any combination thereof.

IPC 8 full level (invention and additional information)

C12Q 1/68 (2006.01)

CPC (invention and additional information)

C12Q 1/686 (2013.01)

Combination set (CPC)

C12Q 1/686 + C12Q 2527/146 + C12Q 2563/159

Designated contracting state (EPC)

AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

EPO simple patent family

WO 2012061442 A1 20120510; AU 2011323507 A1 20130613; CA 2816702 A1 20120510; CN 103443289 A 20131211; EP 2635708 A1 20130911; EP 2635708 A4 20141029; JP 2013541347 A 20131114; SG 190076 A1 20130628

INPADOC legal status


2015-05-20 [18W] APPLICATION WITHDRAWN

- Effective date: 20150415

2014-10-29 [A4] DESPATCH OF SUPPLEMENTARY SEARCH REPORT

- Effective date: 20141001

2014-10-29 [RIC1] CLASSIFICATION (CORRECTION)

- IPC: C12Q 1/68 20060101AFI20140926BHEP

2014-02-05 [DAX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT (TO ANY COUNTRY) (DELETED)

2013-09-11 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 20130529

2013-09-11 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR