EP 2646547 B8 20171025 - RATIONAL ENZYME MINING
Title (en)
RATIONAL ENZYME MINING
Title (de)
RATIONALER ENZYMABBAU
Title (fr)
IDENTIFICATION RATIONNELLE D'ENZYMES
Publication
Application
Priority
- SE 1051254 A 20101129
- SE 2011051449 W 20111129
Abstract (en)
[origin: WO2012074476A1] A method for rational mining for induced enzymes in microbial communities is described. The method is characterized in that a community of microorganisms is provided and that the microbial populations of the community are cultivated in a container under conditions of choice, where the microorganisms are given a defined culturing medium, to eliminate matter deriving from the natural habitat and to allow the microbial community to reach a metabolic steady-state. The method is further characterized in that at least one fraction of microorganisms is taken from the container and transferred into at least two separate containers, where at least one of the fractions of microorganisms is provided with a defined medium which includes an inducing substance and/or a substance against which enzyme/enzymes is/are desired, to induce regulation of expression of the desired enzyme/enzymes, and at least one fraction is provided with a defined medium without said inducing substance and without said substance against which enzyme/enzymes is/are desired, for the purpose of comparison, and the fractions are maintained and/or cultivated. Samples of the two fractions are then withdrawn and analyzed for identification of the induced enzyme/enzymes.
IPC 8 full level
C12N 9/00 (2006.01); C12N 15/01 (2006.01); C12Q 1/02 (2006.01)
CPC (source: EP SE US)
C12N 1/36 (2013.01 - EP US); C12N 9/00 (2013.01 - SE); C12N 15/01 (2013.01 - SE); C12Q 1/02 (2013.01 - EP SE US); C12Q 1/25 (2013.01 - US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
DOCDB simple family (publication)
WO 2012074476 A1 20120607; CA 2819222 A1 20120607; DK 2646547 T3 20171113; EP 2646547 A1 20131009; EP 2646547 A4 20140430; EP 2646547 B1 20170816; EP 2646547 B8 20171025; SE 1051254 A1 20120530; SE 535469 C2 20120821; US 2013280731 A1 20131024; US 9365887 B2 20160614
DOCDB simple family (application)
SE 2011051449 W 20111129; CA 2819222 A 20111129; DK 11844020 T 20111129; EP 11844020 A 20111129; SE 1051254 A 20101129; US 201113989513 A 20111129